LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2078..2174) /label=attL2 /note="attL2 (c)" misc_signal complement(2189..2208) /note="T7 Promoter/priming site (c)" primer_bind 2216..2232 /note="M13 Reverse priming site" gene 2345..3154 /note="Kanamycin resistance gene" rep_origin 3275..3948 /note="pUC origin" vector join(2086..3951,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2071 /note="BGH01_B01a2" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAAAACCTTT AACCCAGATT TCGTATGGGG 721 AGCAGCCAGT TCCGCCTATC AGGTAGAAGG CGCCACCACC ACCGATGGCA GAGGCCCCAG 781 TATTTGGGAT GCGTTCAGTT CCATTCCCGG TAAAACCTAC CACAACCAAA ACGCCGACAT 841 AGCCTGCGAC CACTACAACC GCTGGCAAGA AGACGTGGCC ATAATGAAAG AGATGGGGCT 901 AAAGGCTTAC CGCTTTTCTA TTTCTTGGTC GCGCATATTC CCTACTGGGC GCGGCGAAGT 961 TAACGAAAAA GGCGTAGCCT TTTACAACAA CCTTATCGAC GAATTAATAA AAAACGACAT 1021 TACCCCTTGG GTAACCCTAT TTCACTGGGA CTTTCCTCTG GCACTGCAAA TGGAAATGGA 1081 CGGCCTACTT AACCCCGCCA TCGCCGACGA ATTCGCCAAC TACGCCAAGC TGTGTTTCGC 1141 GCGCTTTGGC GACCGCGTTA CCCACTGGAT TACCCTAAAC GAACCTTGGT GCAGTGCCAT 1201 GCTTGGCCAC GGCATGGGCA GCAAAGCCCC TGGCCGCGTA TCTAAGGATG AACCCTATAT 1261 AGCCGCCCAC AACTTGCTGC GTGCACACGG CAAAATGGTA GATATTTACC GGCGCGAATT 1321 TCAGCCCACA CAAAAAGGCA TGATAGGCAT AGCCAACAAT TGCGACTGGC GCGAACCCAA 1381 AACCGATTCT GAATTAGATA AAAAAGCAGC CGAGCGCGCC CTAGAATTTT TTGTAAGCTG 1441 GTTTGCCGAC CCCATTTATT TGGGCGACTA CCCAGCCAGC ATGCGCGAGC GCTTGGGTGA 1501 GCGTTTACCC ACCTTTAGCG ACGAAGACAT TGCGCTAATA AAAAACTCTA GCGACTTTTT 1561 TGGTTTGAAT CACTACACCA CCATGCTTGC CGAACAAACC CACGAAGGTG ACGTTGTTGA 1621 AGATACTATT CGCGGCAACG GCGGCATATC GGAAGACCAA ATGGTCACCC TCTCCAAAGA 1681 CCCAAGCTGG GAACAAACCG ACATGGAGTG GAGCATTGTG CCCTGGGGCT GTAAAAAATT 1741 ATTAATCTGG TTAAGCGAGC GCTACAACTA CCCCGACATT TACATTACCG AAAACGGCTG 1801 CGCCCTACCC GACGAAGACG ACGTAAACAT AGCCATTAAC GATACACGCC GCGTAGATTT 1861 TTACCGCGGT TATATCGATG CGTGTCACCA AGCAATAGAG GCCGGCGTAA AACTAAAAGG 1921 CTATTTTGCA TGGACACTTA TGGATAACTA CGAATGGGAA GAAGGCTACA CCAAACGCTT 1981 TGGCTTAAAC CATGTAGATT TCACCACAGG CAAACGCACA CCTAAACAGT CTGCAATTTG 2041 GTATAGCACG TTAATTAAAG ATGGTGGGTT CTAGGACCCA GCTTTCTTGT ACAAAGTTGG 2101 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 2161 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 2221 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 2281 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 2341 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 2401 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 2461 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 2521 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 2581 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 2641 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 2701 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 2761 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 2821 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 2881 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 2941 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 3001 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 3061 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 3121 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 3181 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 3241 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3301 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3361 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3421 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 3481 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3541 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3601 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3661 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 3721 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 3781 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 3841 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 3901 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //