LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2108..2204) /label=attL2 /note="attL2 (c)" misc_signal complement(2219..2238) /note="T7 Promoter/priming site (c)" primer_bind 2246..2262 /note="M13 Reverse priming site" gene 2375..3184 /note="Kanamycin resistance gene" rep_origin 3305..3978 /note="pUC origin" vector join(2116..3981,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2101 /note="BGH01_B01b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cTTTCCTCTA GGTTATAATT ATATTATTAC 721 ACTGTTTGCA AATAATATCT TAAAGGGTGT GGTAAACATG TCAAAGATAA CTTTCCCAAA 781 AGATTTCATA TGGGGTTCTG CAACAGCAGC ATATCAGATT GAAGGTGCAT ACAACGAAGA 841 CGGCAAAGGT GAATCTATAT GGGACCGTTT TTCCCACACG CCAGGAAATA TAGCAGACGG 901 ACATACCGGC GATGTTGCAT GCGACCACTA TCATCGTTAT GAAGAAGATA TCAAAATAAT 961 GAAAGAAATC GGTATTAAAT CATACAGGTT TTCCATCTCA TGGCCCAGAA TCTTTCCTGA 1021 AGGAACAGGT AAATTAAATC AAAAGGGACT GGATTTTTAC AAAAGGCTCA CAAATCTGCT 1081 TCTGGAAAAC GGAATTATGC CTGCAATCAC TCTTTATCAC TGGGACCTTC CCCAAAAGCT 1141 TCAGGATAAA GGCGGATGGA AAAACCGGGA CACCACCGAT TATTTTACAG AATACTCTGA 1201 AGTAATATTT AAAAATCTCG GAGATATCGT TCCAATATGG TTTACTCACA ATGAACCCGG 1261 TGTTGTTTCT TTGCTTGGCC ACTTTTTAGG AATTCATGCC CCTGGGATAA AAGACCTCCG 1321 CACTTCATTG GAAGTCTCGC ACAATCTTCT TTTGTCCCAC GGCAAGGCCG TGAAACTGTT 1381 TAGAGAAATG AATATTGACG CCCAAATTGG AATAGCTCTC AATTTATCTT ACCATTATCC 1441 CGCATCCGAA AAAGCTGAGG ATATTGAAGC AGCGGAATTG TCATTTTCTC TGGCGGGAAG 1501 GTGGTATCTG GATCCTGTGC TAAAAGGCCG GTATCCTGAA AACGCATTGA AACTTTATAA 1561 AAAGAAGGGT ATTGAGCTTT CTTTCCCTGA AGATGACCTG AAACTTATCA GTCAGCCAAT 1621 AGACTTCATA GCATTCAACA ATTATTCTTC GGAATTTATA AAATATGATC CGTCCAGTGA 1681 GTCAGGTTTT TCACCTGCAA ACTCCATATT AGAAAAGTTC GAAAAAACAG ATATGGGCTG 1741 GATCATATAT CCTGAAGGCT TGTATGATCT GCTTATGCTC CTTGACAGGG ATTATGGAAA 1801 GCCAAACATT GTTATCAGCG AAAACGGAGC CGCCTTCAAA GATGAAATAG GTAGCAACGG 1861 AAAGATAGAA GACACAAAGA GAATCCAATA TCTTAAAGAT TATCTGACCC AGGCTCACAG 1921 GGCAATTCAG GACGGTGTAA ACTTAAAAGC ATACTACTTG TGGTCGCTTT TGGACAACTT 1981 TGAATGGGCT TACGGGTACA ACAAGAGATT CGGAATCGTT CACGTAAATT TTGATACGTT 2041 GGAAAGAAAA ATAAAGGATA GCGGCTACTG GTACAAAGAA GTAATCAAAA ACAACGGTTT 2101 TTAGGACCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG 2161 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT 2221 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC 2281 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA TCATCATGAA CAATAAAACT 2341 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC 2401 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 2461 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA 2521 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 2581 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 2641 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA 2701 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 2761 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 2821 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 2881 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA 2941 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 3001 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 3061 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 3121 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 3181 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA 3241 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC 3301 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3361 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3421 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 3481 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3541 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3601 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 3661 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 3721 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 3781 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 3841 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 3901 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 3961 CTGGCCTTTT GCTCACATGT T //