LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2315..2411) /label=attL2 /note="attL2 (c)" misc_signal complement(2426..2445) /note="T7 Promoter/priming site (c)" primer_bind 2453..2469 /note="M13 Reverse priming site" gene 2582..3391 /note="Kanamycin resistance gene" rep_origin 3512..4185 /note="pUC origin" vector join(2323..4188,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2308 /note="BGH13_B04a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cACGACAACC CCCTGGTGGC GCGGCGCGGT 721 TATTTATCAG GTATACCCCC GCAGTTTGAT GGATACCAAT GGCGATGGCA TTGGTGATAT 781 TCCCGGCATT ATCAAAAAGC TCGACTACAT CGCCAGCCTG GGTGTGGACG CCATTTGGGT 841 ATCGCCATTT TTCAAGTCGC CGATGAAAGA CTTCGGTTAC GACATTAGTG ACTATCGGGA 901 TATTGATCCC ATCTTTGGAA CCCTGAGTGA TTTTGATGAG CTGATCAGCA AAGCCCATGC 961 GCGCAATATC AAGATTATTA TCGATCAGGT GCTCAGCCAT ACCTCGGACC AGCATGCCTG 1021 GTTTTTGGAG AGCCGCGAGT CGCGCGACAA CCCGAAAGCT GATTGGTATG TGTGGGTAGA 1081 TCCTCAACCC GACGGAACCC CCCCCAACAA TTGGTTGGCA ATTTTTGGGG GCTCTGCCTG 1141 GGAGTGGGAG CCGCGTCGTT GTCAATATTA CCTGCATAAC TTTTTGCGTA CCCAGCCAGA 1201 TTTAAATTAC CACTGTCCGC AAGTGCGCGA GCAAATTTTG CAGGAAGTGG AGTTCTGGTT 1261 GAAACGCGGC GTGGATGGTT TGCGCCTTGA TGCCATTAAC TTCTGCTTTC ACGATAAGGA 1321 ATTGCGTTCC AATCCTGCCA AGCCGGAAGC AGAGCGTAAG GGGCGAGGCT TTAAGGAAGA 1381 CAACCCCTAC GCATTCCAGA AACACATATT TGATAATACC CGCCCGGAAA ACCTGGCTTT 1441 CCTGGAGTCC TTGCGCCAGT TAATGGATCG TTATCCCGGG ACGGTAACCC TCGGTGAAAT 1501 TTCAGCCGAG GACTCCCTGA AAACCATGGC GGAATACACC TCCGGTAACG ACCGTTTGCA 1561 TATGGCGTAT AGCTTTGAAC TGCTGGTCGA TAAGTTATCC GGCAACTATA TCCGCAATAC 1621 GGTGGAAACC CTGGAAGCCA ATTTGGCTGA GGGCTGGCCC TGTTGGTCTA TAGGCAACCA 1681 CGATGTGGTG CGATTTATGT CGCGTTGGGG CGGTGAGGCA CAATCCCCCG AACTCGCCAA 1741 AACCCTCAAT GCCATGCTGC TTTCATTGCG CGGCAGTGTG TGTAGCTATC AGGGGGAAGA 1801 ACTGGGTTTG ACAGAAGCGG ATATCCAGCA GCATGAGTTG CAAGATCCCT ATGGTATTAC 1861 CTTCTGGCCT CGCTTCAAGG GGCGCGATGG ATGTAGAACG CCAATGCCCT GGGACTCATC 1921 CCTGGAATAC GCGGGATTTT CCAGTGCAAA ACCATGGCTA CCCGTATCGG CAAAACACCT 1981 GGCGCTCTCC ATCGATAAAC AGGAAAGCAA TCAGGCATCT GTGCTGAACG CCTATCGCCA 2041 TTTTATGCAG TGGCGTAAAA CGCAAGCTGC CCTGCGCTTT GGTGGCATTG AATTTCTCTC 2101 CAGTAGTGAA TCTCATTTGG TGTTTGTTCG TCGCCATGGC AATGAGGCGC TATTGTGTGG 2161 ATTTAATTTC AGTGCCCAGC CTGCCGTTAT TGAGTTGGGA CAAGTGTTGG GCCTGGAGTC 2221 AATAACCGGC CATGGTTTTG CAACGCAATC CTCCCGGTCA AACAATATTG GTTTGCCGGG 2281 GTATAGCGCG TTTTTTGCCA GCCTTGTCTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 2341 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 2401 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 2461 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2521 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2581 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2641 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2701 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2761 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2821 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2881 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2941 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 3001 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 3061 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 3121 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 3181 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 3241 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 3301 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 3361 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 3421 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3481 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3541 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3601 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3661 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3721 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3781 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3841 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3901 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3961 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 4021 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4081 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4141 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //