LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3215..3311) /label=attL2 /note="attL2 (c)" misc_signal complement(3326..3345) /note="T7 Promoter/priming site (c)" primer_bind 3353..3369 /note="M13 Reverse priming site" gene 3482..4291 /note="Kanamycin resistance gene" rep_origin 4412..5085 /note="pUC origin" vector join(3223..5088,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..3208 /note="BGH02_B01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCAAGGTAAT GACACTTCGG AAGTCATGCT 721 ACTGGATACC GGTTGGGAAT TTTCTCAATC AGGGACTGAA AAGTGGATGC CAGCCACGGT 781 TCCTGGTACG GTTCATCAGG ACCTGATCAG TCATGAATTA CTGCCGAATC CTTTTTATGG 841 GATGAATGAA AAGAAAATTC AATGGGTGGA GAATGAGGAC TGGGAATATA GAACCTCCTT 901 TATAGTCTCC GAAGAGCAAT TGAATCGTGA CGGAATCCAA CTTATTTTTG AAGGTCTTGA 961 TACCTATGCA GACGTATATC TCAACGGCTC ATTACTGCTG AAGGCTGATA ATATGTTTGT 1021 GGGTTATACA CTTCCTGTAA AATCTGTGTT GCGGAAGGGC GAGAATCACT TATATATTTA 1081 TTTCCATTCA CCTATTCGGC AAACGTTGCC ACAATATGCT TCCAATGGCT TCAATTATCC 1141 GGCGGATAAC GATCATCACG AGAAACATCT TAGTGTGTTC AGCCGTAAGG CTCCGTATAG 1201 TTACGGTTGG GACTGGGGCA TCCGAATGGT GACAAGTGGT GTCTGGCGCC CGGTTACTCT 1261 TCGTTTTTAC GACATAGCTA CCATATCCGA CTACTATGTC AGACAATTAT CGCTGACAGA 1321 TGAAAACGCT CGTCTGTCTA ATGAGCTGAT TGTAAACCAG ATCGTTCCTC AGAAAATTCC 1381 GGCTGAAGTA AGGGTCAATG TATCGTTGAA TGGAACTACC GTTACAGAAG TAAAACAGCA 1441 GGTGACACTG CAACCCGGGA TAAATCATAT CACTCTTCCT GCTGAAGTGA CGAACCCTGT 1501 ACGATGGATG CCAAATGGTT GGGGAACTCC GACTCTGTAT GATTTCTCGG CTCAGATAGC 1561 TTGTGGAGAT CGGATTGTTG CGGAACAATC TCATCGGATT GGTTTACGAA CGATTCGTGT 1621 GGTAAATGAA AAAGATAAGG ACGGAGAATC TTTTTATTTT GAAGTCAATG GCATACCGAT 1681 GTTTGCCAAA GGAGCTAATT ATATTCCTCA GGATGCACTG CTGCCTAATG TTACTACGGA 1741 ACGCTATCAG ACTTTGTTCC GTGACATGAA GGAGGCCAAT ATGAATATGG TTCGTATATG 1801 GGGTGGTGGG ACATATGAGA ATAATCTTTT CTATGACCTT GCCGATGAGA ACGGGATACT 1861 TGTCTGGCAG GATTTTATGT TTGCGTGTAC CCCTTATCCA TCCGATCCTA CTTTCCTGAA 1921 ACGGGTAGAG GCGGAGGCTG TTTACAATAT TCGTCGTCTT CGGAATCATG CCTCTTTGGC 1981 GATGTGGTGT GGTAATAATG AAATACTGGA AGCTCTGAAA TATTGGGGTT TTGAGAAGAA 2041 GTTTACTCCG GAGGTTTATC AAGGATTAAT GCATGGTTAT GATAAACTGT TCCGTGAGCT 2101 GTTGCCTTCA ACGGTTAAAG AATTTGATTC GGATCGTTTT TATGTGCACA GTTCGCCTTA 2161 TCTCGCTAAC TGGGGGCGTC CGGAGTCATG GGGGACAGGA GACAGTCATA ATTGGGGAGT 2221 CTGGTACGGA AAGAAGCCAT TTGAGTCTTT AGATACCGAT TTGCCGCGTT TTATGAGTGA 2281 ATTTGGTTTC CAGTCATTTC CGGAGATGAA AACTATTGCT GCTTTTGCTG CTCCTGAAGA 2341 TTATCAGATT GAGTCTGAAG TGATGAATGC GCATCAGAAG AGTAGTATTG GTAATTCGTT 2401 GATCCGTACT TATATGGAAC GTGATTATAT CATTCCCGAA AGTTTCGAGG ATTTTGTGTA 2461 TGTCGGATTA GTCTTGCAAG GACAGGGGAT GCGGCATGGA CTGGAAGCTC ACAGACGCAA 2521 TCGTCCTTAT TGTATGGGGA CATTGTATTG GCAGTTGAAC GACAGTTGGC CGGTAGTATC 2581 CTGGTCAAGT ATTGATTATT ATGGTAATTG GAAGGCATTA CATTATCAGG CAAAGCGTGC 2641 TTTTGCACCG GTCTTGATCA ATCCTATACA GCAGAATGAC AGTTTGAGTG TCTATCTGAT 2701 CTCAGACCGG CTTGATACAA TGGAGCAAAT GACATTGGAA ATGAAAGTCG TGGATTTTGA 2761 TGGAAAAACG CTGGGTAAGA AGATACAAGT ACATTCGTTG GAAGTTCCGG CAAATACTTC 2821 AAAATGTGTT TATCGTGCAA AACTGGATGG CTGGCTGACT CCCGAAGACT GCCGACGTAG 2881 CTTCTTGAAA TTGATACTCA AAGATAAATC GGGACATCAG GTGGCTGAGA GCGTCCATTT 2941 TTTCAGAAAG ACGAAAGATT TGCAACTTCC TCCGACATCA GTATCCTATC AAATGAAGCA 3001 GACGGATGGT AAATGTGAAT TGACTCTCTT TTCATCAATG TTGGCAAAAG ATATTTTTAT 3061 TGAGACTCCA TTGCAAGGAG CCCGTTACAG TGACAACTTC TTTGATCTCT TACCCGGAGA 3121 ACGTAAGAAA GTAATTATCA CTTCTCCCCG GATAAAGAAG GGAGAAGAAT TACCTGTCAA 3181 TATCAAGCAT ATTCGAGAAA CCTATAAATA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 3241 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 3301 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 3361 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 3421 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 3481 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 3541 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 3601 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 3661 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 3721 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 3781 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 3841 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 3901 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 3961 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 4021 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 4081 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 4141 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 4201 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 4261 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 4321 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 4381 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 4441 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 4501 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 4561 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 4621 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 4681 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 4741 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 4801 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 4861 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 4921 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4981 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 5041 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //