LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2429..2525) /label=attL2 /note="attL2 (c)" misc_signal complement(2540..2559) /note="T7 Promoter/priming site (c)" primer_bind 2567..2583 /note="M13 Reverse priming site" gene 2696..3505 /note="Kanamycin resistance gene" rep_origin 3626..4299 /note="pUC origin" vector join(2437..4302,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2422 /note="BGH02_B01c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCTTTATCCT AAAGAGAGTG AAACAAGAGA 721 GGTAAAGGAT TTGTCAGGAG TGTGGGAGTT TAGAACCGAA TCTGATAAAA ATTATATCCT 781 CATGCCAGTT CCTGCAAGTT TTAACGACAT CACACAAGAT ATTAATTTAA GAGATTATGT 841 TGGCAGAGTA TATTATAAAA AATCCTTCTT TATACCGGTT TATTGGAAAG AAAGAAATAT 901 TTTTTTGAGG GTGGGAGCAG CAGCTCATTT TTCTGAGGTT TATGTAAATG GTAATTTAGT 961 GACTAAACAC AAAGGAGGAT TTCTTCCTTT CGAGGCAGAG ATCTCTAAAT TTGTAAATTA 1021 TGGACAAGAA AATATTATAG AAATTATGGT AGACAACACT TTAACTTGGG ATGTTCTTCC 1081 TCCTGGAGAA TTAAAAGTTA TTGAGGACGA AATGCATCCT AAAGGATATA AGGTTTTGAA 1141 TTATTATTTT GATTTTTTTA ATTACTCTGG AATTCACAGG CCTGTGGTAA TATATACCAC 1201 TCCTAAGGTA TATATTAAAG ATTTTTCTGT TATTACTGAA CTAAGTAATA ATTCTGCATT 1261 GGTAAAGTAC AGTATTGAAA GTGAAGGTAA TAATTTTCAA GTTATTTTAA GAGATAAAGA 1321 TAAAAATATA GTAGCAGAGA ATTTTGGTAA ATCAGGAGTT TTAGAGGTAA AAAATCCAAA 1381 ATTATGGGAG CCTGGAAATC CTTATCTATA TAATTTAGAG ATTAAACTTT TGGAAAAAGA 1441 CAACTTTGAT ATTTATAGAA TGGACATAGG AATAAGGACG GTAAGAGTAG AGGGAAAACA 1501 GTTTCTTATA AATGAGAAAC CATTTTACTT TAAGGGTTTT GGTAAGCATG AGGACTCAGA 1561 TATAAGGGGA AAAGGTTTAG ATCAAGTTAT TAACATTAAA GATTTTAATT TATTGAAATG 1621 GATAGGGGCA AACTCTTTTA GAACCTCTCA CTATCCCTAT AGCGAGGAGA TACTTTTCCT 1681 TGCTGACCAA TATGGGATAG CTGTTATTGA GGAAGCTCCA GCTGTTGGGT TAAATTTATG 1741 GAACCGTAAT GAAAAAGTTT TTACTGAAGG AAGGGTTGAT GGAAAGACAT TAGAGCATCA 1801 TTTAGAAATT ATGAGGGAAC TTATAGCGAG GGATAAAAAT CATCCGAGTG TAATTATGTG 1861 GAGCGTAGCT AATGAGGCGG CAACTTACGA AGATGGAGCA GAGGAATATT TTAGGAGAGT 1921 TATAGAGGAA ACAAGAAGGC TCGATCCTAC AAGACCAATA ACCATAGTAG AAAATACTAA 1981 GGCAAGTGAG ACGAAAGTAA GTAAATATGT TGATGTGATA TGTGTTAATA GATACTATTC 2041 ATGGTATACC GATTCGGGAG ATTTAAGTGT TATTGAATAT CAACTTGAAA GAGATCTTAG 2101 AGAGTGGTAT GAATTATACA GAAAGCCGAT AATTCTCTCT GAGTTCGGGG CAGATGCTAT 2161 TTCTGGATTT CATTCTGATC CTCCCTTAAT GTTTACAGAG GAGTATCAAC AAGAGATGAT 2221 TAAAAGATTT GTGGGAGTTC TTGACAGGCT TGATTTTGTT GTGGGAGAGC ATATTTGGAA 2281 TTTTGCTGAT TTTATGACGA AACAGAGTAT AACGAGGGTA GTGGGCAATA AGAAAGGGGT 2341 TTTTACAAGG AATAGACAGC CTAAAATGGT GGCTCATTTT TTGAAAGAGA GATGGAGTAA 2401 ATTACCTGAT TTTTGGGAAA AATAGGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 2461 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 2521 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2581 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2641 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2701 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2761 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2821 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2881 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2941 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 3001 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 3061 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 3121 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 3181 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 3241 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 3301 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 3361 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 3421 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3481 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3541 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3601 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3661 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3721 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3781 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3841 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3901 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3961 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 4021 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4081 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4141 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4201 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4261 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //