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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3155..3251) /label=attL2 /note="attL2 (c)" misc_signal complement(3266..3285) /note="T7 Promoter/priming site (c)" primer_bind 3293..3309 /note="M13 Reverse priming site" gene 3422..4231 /note="Kanamycin resistance gene" rep_origin 4352..5025 /note="pUC origin" vector join(3163..5028,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..3148 /note="BGH02_B02c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cACATCCTTG CCGAAGAACG GTTTCAACAT 721 CGACCTTTCC GGTTCGTGGC AGCTCGCCTC CGTCGACGGC GAGATCCGCA CCGCGATCAT 781 GCTGCCGGGC GACGTGCACA CGGCTCTGCA CCGGGCAGGA TTGATCCCCG ATCCCTATTT 841 CGGCCGCAAC GAGGAAAAGG TGCAGTGGGT CGCCGAGCGC GAATGGGCGG TCGAGCGCAG 901 CTTCGCGCTT CAGGAGGTCG AGGGCGACTG GTATCTCGAT ATCGATTATC TCGACACGGT 961 TGCCAGCGTC CACGTCAACG GCTTTCTGGC GCTCGAAGCC GACAACAGCT TCCGCCGCTA 1021 CCGGCCCGAT GTCTCCAGCA TGCTGAAATC AGGCGATAAC AGCATCCGCA TCGTCTTTGC 1081 CTCCAACATC GCTGTCGGTG CCGCGCGACA GAAGCAGCAG CCCTTCTACA TTCCCTACAG 1141 CACGGGCAAC TCACCGATCC CCGACGGCAA CATGCTGCGC AAGCCGCAAT GCCATTTCGG 1201 CTGGGACTGG AACATCGCCA TTGCCCCGCT CGGTCTTTAC GGAACGATCG CGCTGAAAAA 1261 GCTCGAAACG GCGCGCATCG AACACGTCGT CACCCGCCAG ACCCACAATA ATGACGGTTC 1321 GGTCGACCTC AAAGTGACGG CGACGCTGTT TTCCAAGGGG CCAGACATTG CCCAGGTCTA 1381 TTTCGACCTC GATGGCGAGC GCGTGCGCCT GGATGTCGGC GTCAATGGCG AGACCCATGT 1441 CAACCATCTC TTCCATATCG ACAATCCGCG CCTCTGGTGG CCCTCCGGCA GCGGCGAGCA 1501 GGCGCTCTAC AGGCTTTCCG TCGAATTGCC GACGGATGAG GTGACCAAGC AGATCGGTCT 1561 TCGCACCATC GAGCTGATCA CCACGCCGGA TGCGGCCGGC AGCCGCTTTG CCTTCAAGGT 1621 CAACGGCCGC GAGATCTTTT GCCGCGGCGC CAACTGGATC CCGGCCGACG CGCTGTTTTC 1681 GCTGTCTTCA CCTGAGAAGA CCGAAGACCT GCTGCAATCG GCCAAAGCCG CCAACATGAA 1741 CATGATCCGT GTCTGGGGCG GCGGCTTCTA TGAGCAGGAT CATTTCTACG ATCTCTGCGA 1801 CCGGCTGGGC CTTATGGTCT GGCAGGACTT CATGTTTGCC TGCAACCTCT ATCCGTCGAC 1861 CGAGGACTTT CTCGACAATG TGACGATCGA GGTCGACTAC CAGGTGCGCC GGCTCTCCTC 1921 GCATGCCTCG ATCGTGCTCT GGTGCGGCGA CAATGAACTG GTCGGAGCGC TCACCTGGTT 1981 CGAGGAATCG CGGAAGGATC GCGACCGCTA TCTCGTCTCT TATGATCGCC TCAACCGCAC 2041 GATCGAGCAG GCAGTGAAAA AGGCGCTGCC CGGCGCGGTC TGGTGGCCGT CGAGCCCGGC 2101 CTCCGGCTAT CTCGATTTCG GCGATGCCTG GCATGCGGAC GGGTCCGGCG ACATGCATTA 2161 CTGGTCGGTC TGGCACGAGA ACAAGTCGTT CGACAATTAC CGCTCGGTGC GTCCGCGCTT 2221 CTGCTCGGAA TTCGGCTTCC AGTCCTATAC GTCGCTGCCT GTGATCAAGA CCTATGCCGA 2281 GGCGAAAGAC ATGAACGTCG CTTCCCCGGT CATGGAGCTG CACCAGAAGA ATGCCGGCGG 2341 CAACGAGCGC ATCGCCGGCA CGATGTTCCG TTATTTCCGA TTCCCCAAGG ATTTCCCGAA 2401 CTTCGTCTAT CTCTCCCAGA TCCAACAGGG GCTGGCGATC AAGACGGCGG TGGAATACTG 2461 GCGGTCGCTC AAACCCCATT GCATGGGCAC GATCTACTGG CAGCTCAACG ACACCTGGCC 2521 GGTCGCCTCC TGGTCGAGCC TCGACTATGG CGGCCGCTGG AAGGCGATGC ACTATCTCGT 2581 CAAGCGCTTC TTCCAGCCGG TCGCGGTCGC GGCCATTCCT TCCGACGACG GAAAGACGAT 2641 TCGCTTCTCG CTGGTCAACG ACACGCTTGC CGATGTCAGC GTCGACCTTT CGATCTCGCT 2701 GCTGACGATG CAGGGCGAAC GCCGGCACCT GAGAGACGTA CAGGCCATAT GTTCGCCGGA 2761 TGCGGCCGTC ACTGCCACGA GCATCGACGT TTCCGATATT CCCGAGGGAA CGTTGCTTGC 2821 CTGGCGTTTC ACCGCGTCGA ACGGCACGGG CGGCGAGGGG CACCATGTCA ACGGTACCTA 2881 CAAGGCGCTG GAGCTGGAAC CCGCGGGGCT GACAGTCACC CACGAATATG TCGAGGAGAA 2941 TGGCTCGGTC GACATCAACG TCACCGCGAA GGGACTTGCG CTTTTCGTGA TGATCGAAAC 3001 CGAGACCGAC GGCAAATATT CCGACAACGC CTTCGATCTT GCGGCCGGCG AGAGCCGCCG 3061 CATCGCCTTT ACCCCGGCAC GGCCGCTCGA TCGTGGCGCG CTTCCGGAGT TCCGGTTCTA 3121 CGACCTGTAT TCCTGCCAGT CGGCGGATTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 3181 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 3241 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 3301 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 3361 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 3421 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 3481 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 3541 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 3601 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 3661 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 3721 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 3781 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 3841 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 3901 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 3961 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 4021 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 4081 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 4141 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 4201 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 4261 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 4321 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 4381 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 4441 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 4501 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 4561 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 4621 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 4681 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 4741 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 4801 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 4861 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4921 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4981 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //