LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2042..2138) /label=attL2 /note="attL2 (c)" misc_signal complement(2153..2172) /note="T7 Promoter/priming site (c)" primer_bind 2180..2196 /note="M13 Reverse priming site" gene 2309..3118 /note="Kanamycin resistance gene" rep_origin 3239..3912 /note="pUC origin" vector join(2050..3915,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2035 /note="BGH29_B01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cATTTCTATG AAACCCCGTT ACAAACCTGA 721 CTGGGAATCT CTGAGGGAAC ACACAGTACC GAAATGGTTC GACAAGGCGA AATTCGGGAT 781 CTTCATTCAC TGGGGGATTT ACTCTGTTCC GGGATGGGCG ACGCCCACCG GAGAACTCGG 841 TAAAGTGCCG ATGGATGCCT GGTTCTTCCA GAATCCGTAC GCAGAGTGGT ACGAAAATTC 901 CCTCAGGATC AAGGAGAGTC CCACCTGGGA ATACCACGTG AAGACCTACG GAGAAAATTT 961 CGAGTACGAG AAGTTTGCGG ATCTTTTCAC CGCAGAGAAG TGGGATCCAC AAGAGTGGGC 1021 TGATCTCTTC AAAAAAGCAG GAGCGAAGTA CGTGATACCG ACAACGAAAC ACCACGATGG 1081 ATTTTGTCTG TGGGGGACGA AATACACAGA TTTCAACTCC GTGAAGAGAG GACCGAAGAG 1141 AGATCTCGTA GGAGATCTTG CAAAAGCCGT AAGAGAAGCA GGATTGAGAT TTGGAGTGTA 1201 CTACTCAGGA GGTCTGGACT GGCGCTTCAC GACCGAGCCG ATAAGATACC CCGAGGATCT 1261 CTCCTACATC AGGCCGAACA CTTACGAGTA CGCAGATTAT GCCTACAAAC AGGTCATGGA 1321 ACTTGTGGAT CTGTACCTTC CCGACGTTCT CTGGAACGAC ATGGGCTGGC CGGAGAAAGG 1381 AAAGGAAGAC CTGAAGTATC TCTTCGCTTA CTACTACAAC AAACATCCAG AAGGTTCTGT 1441 GAACGACAGG TGGGGAGTGC CGCACTGGGA TTTCAAAACG GCCGAGTACC ACGTGAACTA 1501 TCCGGGGGAT CTGCCGGGCT ACAAATGGGA GTTTACGAGG GGAATAGGGC TCTCTTTTGG 1561 ATACAACCGA AACGAGGGGC CGGAACACAT GCTCTCTGTT GAACAGCTCG TCTACACACT 1621 CGTGGACGTT GTGAGCAAGG GAGGAAATCT CCTTTTGAAC GTTGGGCCAA AGGGTGACGG 1681 AACGATTCCG GATCTGCAAA AAGAAAGACT CCTGGGCCTT GGTGAATGGC TGAGAAAGTA 1741 CGGAGATGCC ATCTACGGTA CTTCTGTCTG GGAAAGGTGC TGTGCGAAGA CTGAGGATGG 1801 AACAGAGATC AGGTTCACCA GAAAATGTAA CAGAATCTTT GTCATCTTTC TCGGTATCCC 1861 GACCGGAGAA AAAATTGTAA TTGAGGATCT CAATCTATCA GCAGGGACAG TGAGACATTT 1921 CCTGACGGGG GAGAGATTGA GCTTCAAAAA TGTGGGAAAG AACCTGGAAA TCACAGTACC 1981 CAAAAAGCTC CTTGAAACAG ACAGCATAAC ACTCGTGTTG GAGGCGGTGG AAGAATAGGA 2041 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2101 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2161 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2221 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2281 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2341 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2401 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2461 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2521 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2581 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2641 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2701 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2761 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2821 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2881 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2941 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3001 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3061 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3121 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3181 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3241 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3301 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3361 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3421 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3481 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3541 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3601 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3661 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3721 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3781 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3841 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3901 TTTTGCTCAC ATGTT //