LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2123..2219) /label=attL2 /note="attL2 (c)" misc_signal complement(2234..2253) /note="T7 Promoter/priming site (c)" primer_bind 2261..2277 /note="M13 Reverse priming site" gene 2390..3199 /note="Kanamycin resistance gene" rep_origin 3320..3993 /note="pUC origin" vector join(2131..3996,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2116 /note="BGH29_B02b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCAAAACAAT ATCTACCAAC CAACATGGGA 721 ATCGCTCGAT ACTCGCCCTA CCCCTGCATG GTTCAAAAAT GCAAAATTTG GAATCTTTAT 781 CCATTGGGGA ATCTATTCTG TACCCATGTG GTCACCTAAA GGCACTTACG AAGAATGGTA 841 TAAATATTGG CTGGATAATA AAAAGTTAAT GGGGAACGGT GAATTTACCG GAACCGAAAT 901 TTATGATTAT CATACAAAAA TGTATGGAGA AGACTTTAAT TATGCAGATT TTGCTGCCAA 961 ATTTAAAGCA CATGATTACG ATGCAAACGC TTGGGCCAAA CTATTCGAAA AATCAGGTGC 1021 CAAGTATGTA GTTTTAACAA CTAAACACCA TGACGGTTAT GCATTATGGC CAAGTATGGA 1081 AGCGTCTAGA GATTACGGCA GACCTTGGAA CAGTATGGAA ATAGGTCCTA AACGCGATTT 1141 GGTAGGTGAA TATACCACAG CTCTTAGAAA AACAGACATT AAGGTGGGAT TCTACATTTC 1201 ATGCAGAGAA TGGGGAAGTC CGTTATATTG CCCGGAGTTA CTTAATATAT ATGTATCCCG 1261 TCATTTTATA CCGCAAGTTA AAGATCTGGT AAATCGGTAT GAACCCGATA TTCTTTGGTC 1321 AGACGGTCCC GATAATTACA GTGACTCTAT TTGGCAAACA AAAAAACTTT TCCAATGGCT 1381 CTATTCTGAA TCCCCAGTAA AGAACAAAAT TGTACTGAAT GACAGATGGG CAAAATTTAC 1441 TGATGGGAAA AAGCATGGCG ATTATTACAC CCGTGAATAT AGCAATCGCA ATATGCCAGA 1501 GGATAAACCT TGGGAAGAAT GTCGTGGCAT GGGATTCTCT TTCTCTTATA ATCAAAATGA 1561 AGATATAGAA GATTATTCCA GCCCACAAGG ATTAATACTT ACCTTAGTTA ATATTGTAAG 1621 CAAAGGTGGG AACTTACTCT TGGGCATAGG TCCGAATGGA AATGGCAAAA TTCCCCCTAT 1681 CATGCAGGAA CGCTTATTGC AAATGGGAGA ATGGCTAAAA GTAAATGGAG AAGCAATCTA 1741 TGATACTTAC AAATGGAAAC AATCTGAACA ATGGTCTGAA GGTGACAGAA ATTGGAAAGA 1801 TAAAGGAAAA CATTATGTAG GAGGAAACTC TATTTTAAAA CAAACAGTAG ATCCCGATCC 1861 CGGCTTTGCC GTAAAAGAAG CCTTTTTCAC AGCTAAAAAT AATGCAGTAT ATGCCATTCT 1921 GCCTAAATTT CCGGAAAATC AAGTCATACT GAAAGACATT AGAACAAGTT CCAAAACAAA 1981 AATAACTTTA TTAGGATATG ACAAAACATT GAAATGGAAA CAGAATGGAA ATAATATAGT 2041 AATAGAAATT CCCACTATTC CTTACAGCAA AGTTCCTTGT CAGTATGCAT GGACACTTAA 2101 ATTAGAATAT GTTGAATAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2161 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2221 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2281 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2341 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2401 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2461 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2521 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2581 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2641 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2701 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2761 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2821 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2881 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 2941 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3001 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3061 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3121 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3181 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3241 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3301 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3361 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3421 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3481 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3541 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3601 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3661 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3721 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3781 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3841 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3901 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 3961 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //