LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2507..2603) /label=attL2 /note="attL2 (c)" misc_signal complement(2618..2637) /note="T7 Promoter/priming site (c)" primer_bind 2645..2661 /note="M13 Reverse priming site" gene 2774..3583 /note="Kanamycin resistance gene" rep_origin 3704..4377 /note="pUC origin" vector join(2515..4380,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2500 /note="BGH35_B04a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAGCCGGTTA ACATGGAAGG ATCAGAAATA 721 TAGGCTGGAT GGCGAGGAGT TCCGGATTTT GTCGGGGGCG ATTCATTATT TTCGGGTTGT 781 GCCGGAATAT TGGGAGGACA GGCTGCTGAA GCTGAAGGCC TGCGGTTTCA ATACGGTAGA 841 GACGTATATT CCGTGGAACC TGCATGAACC CCGCGAGGGA AGCTTCCGCT TCGACGGGTT 901 TGCGGATGTC GCCCGGTTCA TAGAGACGGC GGGCCGGCTT GGCCTGCACG TGATTGTCCG 961 TCCCAGCCCT TATATATGCG CGGAGTGGGA GTTTGGCGGC TTGCCGGCCT GGCTGCTGAA 1021 ATCGTCCATG GGCTTGCGCT GCATGGATAA TGAATACCTG GAAAAAGTCG ACCGCTACTA 1081 TGACGAGCTT ATTCCGCGCC TGCTGCCGCT GCTGGATTCG CGGGGCGGTC CGATCATTGC 1141 GGTGCAGGTC GAGAATGAAT ACGGCAGCTA CGGCAATGAC ACCGCGTATT TGGCTTACTT 1201 GAGGGATGGC CTGATCCGAC GCGGAGTTGA CTGTCTGCTG TTCACCTCGG ACGGCCCAAC 1261 GGACGAGATG CTGCTGGGCG GCACGGTAGA GGGGCTGCAT GCAACAGTTA ACTTCGGTTC 1321 GCGCGTAGCG GAGTCCCTCG CGAAATACCG GGAGTATAGA CAGGACGAAC CGCTGATGGT 1381 GATGGAGTAC TGGCTTGGCT GGTTCGACCA TTGGCGGAAG CCGCATCATG TCCGGGAGGC 1441 GGGGGATGTC GCTAACGTAT TGGATGAGAT GCTGGAGCAA GGGGCATCCG TGAATCTCTA 1501 CATGTTCCAC GGCGGGACAA ACTTCGGCTT TTACAGCGGA GCCAACTACG GTGAGCATTA 1561 TGAACCAACG ATAACAAGCT ATGATTATGA TGCGCCTCTT ACGGAGTGGG GGGATATAAC 1621 CGAGAAATAC AAAGCTATCC GAAGCGTGCT CGAAAAGCAC GGCATACCGG AAGGAGCGCC 1681 GTTCCCGGCT CCGATTCCGA AGAAAGCTTA CGGCAAGGTT ATTTTAACGG AACGGGGCGA 1741 TTTGCTGGAT CAGCTCGAGC AGGTATCCGC CGAACAGGTG CAATCCGTCA GCATCCGGCC 1801 GATGGAGCAT TACGATCAGG CTTACGGCTT TATTTTGTAC TCGACCCAGG TTAAGGGGCC 1861 AAGAACGAGA CAAAAGCTTC ATCTTCGCGA GGTGCGCGAT CGCGCTCAGG TATTCCTGGA 1921 CGGCAAGCTG ATCGGCGTTG TTGAGCGGTG GAATCCGCAG CCGATTGAGA TCGCGGTTCC 1981 CAGAGAAGGG GCAAGATTGG ATGTCCTGGT CGAAAATATG GGCCGCGTTA ATTACGGCCC 2041 TTATCTGCGC GATCATAAAG GAATAACGGA AGGTATCCTG ATCGATAACC AGTTCCAGTC 2101 GAACTGGACG GTTACCCTGC TGCCGCTTGA GTCCGAGCAG CTCGCTCGGG TCCGCTATGA 2161 GTCTGTGGAA GTAACAGGGG GACAGCAGCA TGACGGGCGT CCTGCCTTCT ATCGTGGATT 2221 TGTGGAGGTG GATGAACCTG CCGATACGTT CCTCCGGTTT GACGGCTGGC AGAAGGGGAT 2281 TGCCTGGATC AACGGCTTCC AGCTTGGCCG CTATTGGGAA GCGGGACCGC AGCGCGCGTT 2341 ATACGTGCCG GGTCCGCTGC TGCGCAAAGG GGAAAACGAA ATTGTTTTAT TCGAGCTGCA 2401 TGGCGTAACC GGACATCCCG AAGTGGATTT AACCGATGCT CCCGACCTTG GCGAGACGGC 2461 TGCGGTTGAC GACCAGGTGC TAAACTTCGT ACAGGAAGAA TAGGACCCAG CTTTCTTGTA 2521 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2581 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2641 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2701 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2761 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2821 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2881 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2941 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 3001 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3061 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 3121 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 3181 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3241 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3301 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3361 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3421 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3481 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3541 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3601 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3661 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3721 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3781 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3841 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3901 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3961 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4021 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4081 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 4141 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4201 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4261 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4321 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //