LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2447..2543) /label=attL2 /note="attL2 (c)" misc_signal complement(2558..2577) /note="T7 Promoter/priming site (c)" primer_bind 2585..2601 /note="M13 Reverse priming site" gene 2714..3523 /note="Kanamycin resistance gene" rep_origin 3644..4317 /note="pUC origin" vector join(2455..4320,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2440 /note="BGH35_B04b" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAGTCCACTG ACTATTCAAG GCAAACAATT 721 AATGCTGAAC GATCGTCCGT TTCGAATTAT AGCCGGCGCC ATCCATTATT TTCGCGTTGT 781 TCCGGAGTAC TGGCGCGACC GCCTGCTTAA ATTGAAGGCA TGCGGGTTTA ATACGGTTGA 841 AACTTACGTG CCGTGGAATT TCCATGAGCC GGAGGAAGGC CGCTTTGTAT TCGAGGGCAT 901 GGCGGATCTG GAGAAGTTCA TCGCGCTTGC CGGCGAACTG GGATTGTACG CGATTGTTCG 961 TCCAAGCCCG TACATTTGCG CGGAGTGGGA GTTTGGCGGA TTGCCGGCAT GGCTGCTCAA 1021 AGACCCCGGC ATGCGCCTGC GCTGCAGCTA TAAGCCGTTC CTGGACAAAG CGGATGCTTA 1081 TTACGACGAA CTGATCCCGC GCCTGACCCC GTTCCTGTCC ACCAAGGGCG GTCCGCTCAT 1141 CGCGATGCAG ATCGAGAACG AGTACGGCAG CTACGGCAAC GATAAAACGT ATCTCAACTA 1201 CCTGAAGGAA GCACTCGTCA AACGCGGCGT GGATGTACTG CTATTTACCT CTGACGGACC 1261 GGAAGATTTT ATGCTGCAAG GCGGCATGGT TGAAGGCGTA TGGGAAACGG TTAATTTCGG 1321 ATCCCGTTCG GCTGAGGCAT TCGCCAAGCT GCAGGAATAT CAGCCGGATC AGCCGCTTAT 1381 GTGCATGGAA TTTTGGAACG GCTGGTTCGA CCACTGGGGG GAGACTCATC ATACCCGCGG 1441 CGCCGCGGAT GTTGCCCTGG TGCTTGACGA GATGCTGGCA GCCGGTGCTT CGGTCAATTT 1501 CTATATGTTC CATGGGGGGA CCAACTTCGG CTTTTTCAGC GGAGCGAACT ACACGGACAG 1561 ACTGCTTCCG ACGGTTACGA GCTACGACTA CGATTCGCCG CTTAGCGAGA GCGGGGAATT 1621 GACGGAGAAA TATTATGCCG TTCGCGAAGT CATTGCCAAG TATGCGGAGC TCGGACCGCT 1681 TGAGCTGCCG GCGCAAATCG TGGCAAAAAG CTTCGGTAGC GTGCGTATGA CTGGCCAAGC 1741 TCGGCTGCTC GCTTCGCTTG ATGAGTTGTC CGTACCGATT CCAAGCGTGT GCCCGGAGCC 1801 GATGGAGCAG TATGGCCAAA ACTCGGGGTT TATCCTGTAC GCGACGCATT TAACCGGCCC 1861 GCGTCCGGCA AGCCGCCTCA ACCTGCAGGA GGTTCATGAC CGCGCGCTTA TCTTTATTGA 1921 CGGCGTGTTC AAAGGCGTTA TTGAACGCTC CAACCCGGAG CATGATCTCG TATTTGACGT 1981 ACCGCCCGGC GGCGTGGAGC TGGCCATTCT CGTCGAGAAT ATGGGACGGA TCAATTACGG 2041 TCCGCACATG AAGGATGTTA AGGGAATTAC CGAAGGCGTA CGTTTCGGAC AGCAGTTCCT 2101 GTTTAATTGG ACGGTGCGCC CGTTGCCGCT GGATGACCTG TCCAAACTGC AATTTTCTGC 2161 TTTAAGCAGT CAACCGTGCC TTCAGCCGTC CTTCTATCGG GGCGAGTTCG AAGTGGATGA 2221 GCCGGCCGAC ACGTTCCTAA GCATGAAAGG GTGGACCAAG GGTGTTGCCT ATATGAACGG 2281 CTTTAATCTG GGTCGTTATT GGGAGATTGC GCCTCAAGAA ACGTTGTACA TTCCGGGGCC 2341 GCTGCTCCGC ACGGGCAAAA ACGAGATCAT CGTATTCGAG CTGCACGCAG CGGAGTCCGC 2401 AAGCGTTAGC CTGCTGGACT GCCCGGTATT AAATAAGCAA TAGGACCCAG CTTTCTTGTA 2461 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2521 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2581 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2641 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2701 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2761 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2821 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2881 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 2941 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3001 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 3061 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 3121 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3181 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3241 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3301 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3361 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3421 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3481 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3541 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3601 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3661 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3721 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3781 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3841 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3901 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3961 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4021 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 4081 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4141 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4201 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4261 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //