LOCUS BGH35_B04b-pET16-DEST 7549 bp DNA circular 17-AUG-2011 FEATURES Location/Qualifiers misc_feature 5323..5352 /note="His10-tag" misc_feature 5365..5376 /note="X-factor" misc_feature 5230..5245 /note="T7 Promoter" misc_feature 5249..5273 /note="Lac O" misc_feature 7276..7337 /note="T7 Terminator" misc_recomb complement(5392..5416) /label=attB1 /note="attB1 site" misc_recomb 7195..7217 /label=attB2 /note="attB2 site" misc_feature complement(3761..4720) /note="Lac I" misc_feature 209..1069 /note="Ap" misc_feature 1224..1843 /note="Ori" misc_feature 5419..5439 /note="TEV site" misc_feature 5440..7188 /note="BGH35_B04b" vector join(7203..7549,1..5399) /source="pET16-DEST" /type="Destination Vector" clone 5400..7202 /source="BGH35_B04b-pDONR221" /type="Entry Clone" ORIGIN 1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt 3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat 5341 catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgctcgaATC AACAAGTTTG 5401 TACAAAAAAG CAGGCTctga aaacttgtac tttcaaggcA GTCCACTGAC TATTCAAGGC 5461 AAACAATTAA TGCTGAACGA TCGTCCGTTT CGAATTATAG CCGGCGCCAT CCATTATTTT 5521 CGCGTTGTTC CGGAGTACTG GCGCGACCGC CTGCTTAAAT TGAAGGCATG CGGGTTTAAT 5581 ACGGTTGAAA CTTACGTGCC GTGGAATTTC CATGAGCCGG AGGAAGGCCG CTTTGTATTC 5641 GAGGGCATGG CGGATCTGGA GAAGTTCATC GCGCTTGCCG GCGAACTGGG ATTGTACGCG 5701 ATTGTTCGTC CAAGCCCGTA CATTTGCGCG GAGTGGGAGT TTGGCGGATT GCCGGCATGG 5761 CTGCTCAAAG ACCCCGGCAT GCGCCTGCGC TGCAGCTATA AGCCGTTCCT GGACAAAGCG 5821 GATGCTTATT ACGACGAACT GATCCCGCGC CTGACCCCGT TCCTGTCCAC CAAGGGCGGT 5881 CCGCTCATCG CGATGCAGAT CGAGAACGAG TACGGCAGCT ACGGCAACGA TAAAACGTAT 5941 CTCAACTACC TGAAGGAAGC ACTCGTCAAA CGCGGCGTGG ATGTACTGCT ATTTACCTCT 6001 GACGGACCGG AAGATTTTAT GCTGCAAGGC GGCATGGTTG AAGGCGTATG GGAAACGGTT 6061 AATTTCGGAT CCCGTTCGGC TGAGGCATTC GCCAAGCTGC AGGAATATCA GCCGGATCAG 6121 CCGCTTATGT GCATGGAATT TTGGAACGGC TGGTTCGACC ACTGGGGGGA GACTCATCAT 6181 ACCCGCGGCG CCGCGGATGT TGCCCTGGTG CTTGACGAGA TGCTGGCAGC CGGTGCTTCG 6241 GTCAATTTCT ATATGTTCCA TGGGGGGACC AACTTCGGCT TTTTCAGCGG AGCGAACTAC 6301 ACGGACAGAC TGCTTCCGAC GGTTACGAGC TACGACTACG ATTCGCCGCT TAGCGAGAGC 6361 GGGGAATTGA CGGAGAAATA TTATGCCGTT CGCGAAGTCA TTGCCAAGTA TGCGGAGCTC 6421 GGACCGCTTG AGCTGCCGGC GCAAATCGTG GCAAAAAGCT TCGGTAGCGT GCGTATGACT 6481 GGCCAAGCTC GGCTGCTCGC TTCGCTTGAT GAGTTGTCCG TACCGATTCC AAGCGTGTGC 6541 CCGGAGCCGA TGGAGCAGTA TGGCCAAAAC TCGGGGTTTA TCCTGTACGC GACGCATTTA 6601 ACCGGCCCGC GTCCGGCAAG CCGCCTCAAC CTGCAGGAGG TTCATGACCG CGCGCTTATC 6661 TTTATTGACG GCGTGTTCAA AGGCGTTATT GAACGCTCCA ACCCGGAGCA TGATCTCGTA 6721 TTTGACGTAC CGCCCGGCGG CGTGGAGCTG GCCATTCTCG TCGAGAATAT GGGACGGATC 6781 AATTACGGTC CGCACATGAA GGATGTTAAG GGAATTACCG AAGGCGTACG TTTCGGACAG 6841 CAGTTCCTGT TTAATTGGAC GGTGCGCCCG TTGCCGCTGG ATGACCTGTC CAAACTGCAA 6901 TTTTCTGCTT TAAGCAGTCA ACCGTGCCTT CAGCCGTCCT TCTATCGGGG CGAGTTCGAA 6961 GTGGATGAGC CGGCCGACAC GTTCCTAAGC ATGAAAGGGT GGACCAAGGG TGTTGCCTAT 7021 ATGAACGGCT TTAATCTGGG TCGTTATTGG GAGATTGCGC CTCAAGAAAC GTTGTACATT 7081 CCGGGGCCGC TGCTCCGCAC GGGCAAAAAC GAGATCATCG TATTCGAGCT GCACGCAGCG 7141 GAGTCCGCAA GCGTTAGCCT GCTGGACTGC CCGGTATTAA ATAAGCAATA GGACCCAGCT 7201 TTCTTGTACA AAGTGGTTGA Ttcgaggatc cggctgctaa caaagcccga aaggaagctg 7261 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg 7321 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg 7381 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac 7441 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga 7501 taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa //