LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2585..2681) /label=attL2 /note="attL2 (c)" misc_signal complement(2696..2715) /note="T7 Promoter/priming site (c)" primer_bind 2723..2739 /note="M13 Reverse priming site" gene 2852..3661 /note="Kanamycin resistance gene" rep_origin 3782..4455 /note="pUC origin" vector join(2593..4458,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2578 /note="BGH35_B04c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cTCTCAATCT TCGGAGGGCA CATTTGAAGT 721 GGGGAAGAAT ACTTTCTTGC TGAATGGAGA ACCGTTTGTT GTAAAAGCGG CAGAGATTCA 781 TTATCCGCGT ATCCCGAAAG AATATTGGGA ACATCGTATT AAAATGTGCA AGGCACTGGG 841 TATGAATACG ATTTGCTTGT ATGTGTTCTG GAATTTTCAT GAACCGGAAG AAGGAAGGTA 901 TGATTTTGCC GGACAGAAAG ATATAGCTGC GTTTTGCCGT TTGGCTCAAG AGAATGGAAT 961 GTATGTCATA GTGCGTCCCG GACCTTATGT CTGTGCGGAA TGGGAAATGG GCGGCTTGCC 1021 TTGGTGGCTG CTGAAGAAGA AGGATATCAA ACTTCGCGAG CAAGACCCAT ACTACATGGA 1081 ACGTGTGAAA CTCTTTTTGA ACGAAGTGGG CAAACAGCTG GCTGATCTTC AGATTAGTAA 1141 AGGAGGGAAT ATCATTATGG TACAGGTGGA GAATGAGTAT GGAGCATTTG GAATCGATAA 1201 GCCGTATATA TCCGAAATCC GTGACATGGT AAAACAAGCC GGTTTTACAG GTGTTCCTTT 1261 ATTCCAGTGT GACTGGAACT CGAATTTTGA GAATAATGCA CTGGATGATT TGCTCTGGAC 1321 TATTAATTTC GGTACGGGTG CTAACATCGA CGAGCAATTT AAACGGTTGA AAGAACTACG 1381 GCCGGACACT CCATTGATGT GCAGTGAATT TTGGTCCGGA TGGTTTGACC ATTGGGGAGC 1441 CAAGCATGAA ACAAGAAGTG CCGAAGAATT GGTAAAAGGT ATGAAAGAAA TGCTGGACCG 1501 CAATATTTCT TTCAGCTTGT ATATGACTCA CGGGGGGACT AGTTTCGGGC ATTGGGGAGG 1561 CGCGAATTTC CCGAACTTTT CACCGACTTG CACATCGTAT GATTATGATG CTCCTATCAA 1621 TGAATCCGGT AAGGTGACTC CTAAATATCT GGAAGTACGG AATCTGCTTG GTAACTACCT 1681 GCCGGAAGGT GAAACCTTGC CGGAAATTCC GGATTCGATA CCGACTATAG CTATTCCTAC 1741 TATTAAAATG ACTGAAATGG CAGTCTTATT TGATAATCTG CCTCACCCCA AAGAGAGTGA 1801 GGATATCCGG ACAATGGAAG CTTTTGATCA GGGATGGGGT AGTATTCTGT ATCGGACTTC 1861 ATTATCCGCT TCTGATAAAG AACAGACATT ACTGATCACA GAAGCTCATG ACTGGGCACA 1921 AGTCTTTCTG AATGGTAAGA AACTGGCTAC TCTGAGCCGT TTGAAGGGGG AAGGTGTAGT 1981 AAAGTTACCA CCATTGAAAG AAGGTGACAG ACTGGATATC CTGGTGGAAG CAATGGGAAG 2041 AATGAATTTC GGCAAAGGTA TTTATGACTG GAAAGGTATT ACCGAGAAAG TAGAACTGCA 2101 ATCTGATAAA GGAGTTGAAC TGGTGAAGGA CTGGCAGGTA TATACTATTC CGGTAGATTA 2161 TAGTTTTGCC CGTGATAAGC AGTATAAACA ACAGGAGAAT GCTGAAAATC AACCGGCTTA 2221 TTATCGGTCG ACGTTTAACC TCAATGAGTT AGGGGATACA TTCTTGAATA TGATGAACTG 2281 GAGTAAAGGT ATGGTATGGG TGAATGGCCA TGCGATTGGT CGTTATTGGG AAATCGGACC 2341 TCAACAGACC TTATATGTAC CGGGCTGCTG GTTGAAGAAA GGAGAGAATG AAATCATTAT 2401 ATTGGATATG GCTGGTCCTT CAAAAGCTGA AACTGAAGGT CTGCGTCAAC CAATTCTGGA 2461 TGTACAACGT GGTAACGGAG CTTATGCTCA TCGTAAGATG GGTGAGAACT TGGACTTAAC 2521 AAATGAAACT CCTGTTTATC AAGGGATATT TAAGTCAGGC AATGGTTGGC AACATGTGTA 2581 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 2641 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 2701 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 2761 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 2821 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2881 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2941 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 3001 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 3061 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 3121 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 3181 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 3241 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 3301 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 3361 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 3421 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 3481 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 3541 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 3601 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 3661 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 3721 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 3781 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3841 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3901 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3961 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 4021 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 4081 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 4141 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4201 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4261 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4321 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4381 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4441 GCCTTTTGCT CACATGTT //