LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2819..2915) /label=attL2 /note="attL2 (c)" misc_signal complement(2930..2949) /note="T7 Promoter/priming site (c)" primer_bind 2957..2973 /note="M13 Reverse priming site" gene 3086..3895 /note="Kanamycin resistance gene" rep_origin 4016..4689 /note="pUC origin" vector join(2827..4692,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2812 /note="BGH89_B01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cCAGCTGGAC AAGGCCGCTA CGGAGGCGTT 721 TTTGAAAAGG GTAGTAAAGG ACAGGGCTAC CGCATTTACC TGCGAATACC TGCCTGCGGA 781 TAACGGAAAG GATGTATTTG AAATAGAAAG TAACGGCAGC CGTATTATGT TGCGCGGAAA 841 CAACGGCGTG AGCGTAGCAT CAGCATTGAA CTACTATCTC AGAAATTACT GTAATTCAAT 901 CATTACCTGG AACGGCACCA ACCTGCACCT GCCGGCTGTA TTGCCGGTTG TGAAAGAGAA 961 AGAGCATCAT GTGACGCCGT ACAGGTACCG TTATTATATC AACTACTGTA CATTCCAGTA 1021 CAGCATGAGC TGGTGGAACT GGGAGAGATG GCAGCAGGAA ATAGACTGGA TGGCGATGAA 1081 TGGTATTAAC ATGCCATTGG CGCTTACCGG TGAAGAAGCG ATCTGGCAGG AAGTGTATAA 1141 AGAGATGGGC TTTACGGATG CAGAACTGGA CAAGTTCTTC AGCGGACCCG CTTATTTCTC 1201 CTGGCTCTGG ATGGGGAATA TCGATGCATG GGGCGGTCCA TTGCCACAAC ACTGGAAAGA 1261 CAGCCATAAG GTATTACAGC AACAGATACT GGCCGCAGAA CGTAGTATGG GTATGTTGCC 1321 AATATTACCA GCCTTTACCG GACACGTACC GCCAGCCTTT AAAGATAAAT ATCCGAATGA 1381 GATCGTAAAG CCGACTAACT GGGACGCTGG TTTCCCGGAT GTATATATCC TCGATCCAAA 1441 CAGTCCGATG TTTGACAAGA TCGGGAAGAA GTTCCTGGAA GCACAGACCA AAGCTTTTGG 1501 TACTGATCAC TTTTACTCAG CGGATACCTT CAATGAAAAT GTGCCGCCTT CGAGCGATTC 1561 TTCATTCCTG GATGCCATGA GTCGTAAGGT ATATGCGTCT ATGGCAGCTG CTGATCCGAA 1621 AGCCGTGTGG GTAATGCAGG GATGGATGTT CCATTATAAT GCGAGTTACT GGCATCAGCC 1681 ACAGATCAGA GCCTTGTTAA ATGCAGTGCC TGATGATCAT ATGATCGTAC TGGATCTGTA 1741 TAGTGAAAGT CATCCTGAAT GGAGGAATAC ACAGGCTTAT TACGGCAAAC CATGGATCTG 1801 GAATATGCTG CACAACTTTG GTGGTAATAC CGGTATGTGG GGTGGAATGG ATGCCGCTGC 1861 ACACGATCCG GCTACTGCGT TGCATGATCC TGCTTCCGGA AAGATGTCGG GTATTGGTCT 1921 GACGCCGGAG GGCATTGAGC AGAATCCTGC GCTGTACCAG CTGATGATTG ATAACGTTTG 1981 GAGAGATCAG CCGATCAACG TAGATACCTG GCTGCAATCG TATGCAAAAC AACGATACGG 2041 TGCAGAGAAT GAGGCAGTAA ACAAGGCATG GCAGATCCTG TATCATACCG TGTATATTGG 2101 CGGTCCGACA GAAGGCGCGC CGGAATCGAT CATTGTTGCA AGACCAACAC TGGATATCGC 2161 AGCAGAGAGA GTAAAGACCA AACTTGAATA TGATCCTGCC AAAGTAGTAC CGGCATGGGA 2221 TCTCTTCATC AATGCAGCCG CACAGCTGAA GCCAACAGAA GGATTTAAGT ATGACCTGGT 2281 AGACCTGACC CGTCAGGTGC TTGGTAACTA TGCCAGTCCA TTACAACAGC GTGTAGCGAC 2341 TGCTTATCGC AATAAAGATC TGGCGGCATT TAAACAATAC AGCACACAAT TCATCGGACT 2401 ACTGGATGAC ATGGATATGC TGCTGGGGAC TCAGGAAGGA TTTCTATTGG GCAAATGGGT 2461 AAGCGATGCG CGCAGTAATG GTATTACGCC GGCAGAGCAG GACCTGTATG AGTTCAATGC 2521 AAAAGACCTG GTGACCTTGT GGGGGGATAA GGATAGTCCG GTACACGAAT ATTCAAACCG 2581 TCAGTGGAAC GGGCTGATTA AAGGCTTTTA TAAGCCTCGT TGGCAGCAGT TCTTCACTTT 2641 GCTGGAGTCC AGCCTCAAAA AGGGCGAAAC AGCCGATTTA AAGGCATTTG AAGAGCAGGT 2701 GAAGGCATTT GAGTGGAAAT GGGCGAACGG GCATGATAAA TATGCCGTAA AGCCACAGGG 2761 AGACGCCGTG AAAGCTGCCG TTCAATTACA CAAGAAATAT CGTAAGATGA TGTAGGACCC 2821 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 2881 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 2941 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 3001 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 3061 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 3121 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 3181 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 3241 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 3301 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 3361 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 3421 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 3481 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 3541 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 3601 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 3661 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 3721 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 3781 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 3841 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 3901 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 3961 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 4021 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 4081 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 4141 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 4201 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 4261 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 4321 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 4381 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 4441 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 4501 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 4561 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4621 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4681 TGCTCACATG TT //