LOCUS BGH89_B01b-pET16-DEST 7945 bp DNA circular 17-AUG-2011 FEATURES Location/Qualifiers misc_feature 5323..5352 /note="His10-tag" misc_feature 5365..5376 /note="X-factor" misc_feature 5230..5245 /note="T7 Promoter" misc_feature 5249..5273 /note="Lac O" misc_feature 7672..7733 /note="T7 Terminator" misc_recomb complement(5392..5416) /label=attB1 /note="attB1 site" misc_recomb 7591..7613 /label=attB2 /note="attB2 site" misc_feature complement(3761..4720) /note="Lac I" misc_feature 209..1069 /note="Ap" misc_feature 1224..1843 /note="Ori" misc_feature 5419..5439 /note="TEV site" misc_feature 5440..7584 /note="BGH89_B01b" vector join(7599..7945,1..5399) /source="pET16-DEST" /type="Destination Vector" clone 5400..7598 /source="BGH89_B01b-pDONR221" /type="Entry Clone" ORIGIN 1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt 3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat 5341 catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgctcgaATC AACAAGTTTG 5401 TACAAAAAAG CAGGCTctga aaacttgtac tttcaaggcC AACGCAATGT AGAAACAGGC 5461 CCTCCTGTAT TAAAAGAAAT GTGCCAAAGA TTATTTCCCC GGCATGCCCA GTCGTTTCTA 5521 TTCGAGTTAC TGACAGATTC TATTGATACA GACCGTTTTA TACTGGAATC GAGTCAAGGG 5581 AAAATACGGA TAAAAGGAAA CAATCGTAAC TCATTGGCTG CCGGTTTGAA TCATTATCTG 5641 AAAAACTACT GTCATACCCA TGTTTCCTGG TATGCCTCAG AGACAGTGGA AATGCCTGAT 5701 GTACTACCGG AGATACCTCA GCCAGTATAT ATCCGTTCCA AATGTGACAA TCGTTTTTTT 5761 CTCAACTATT GTACATTCGG TTATACTATG CCCTATTGGA AATGGCAGGA CTGGGAACGA 5821 CTGATAGACT GGATGGCATT AAACGGAGTC ACCATGCCTC TTGCTATTAC CGGACAAGAA 5881 TCCATTTGGT ACAAAGTATG GACAGACATG GGTTTGAGCG ACGAACAGGT TCGCTCATAC 5941 TTTACAGGGC CAGCCCATCT TCCCTGGCAT CGGATGTCCA ATGTCGATTT CTGGCAAAGT 6001 CCACTACCTC AATCCTGGCT AAAGGATCAG GAGGAATTAC AAAAACGTAT TCTCGAAAGA 6061 GAACGTGAGT TTGATATGAC TCCTGTATTA CCAGCATTTG CCGGTCATGT ACCAGCCGAA 6121 TTAAAAACAA TTTATCCGAA TGCAAAAATC TATCAGATGA GTCAGTGGGG TGGCTTCGAT 6181 GAAAAATACC GCAGTCATTT TATTGATCCG ATGGACTCAT TATACTCAAT CATTCAGCGC 6241 CGTTTTCTGG AGGAACAAAC CAAAGTATAT GGTACTGACC ATATTTATGG AATCGATCCT 6301 TTCAATGAAG TCGACTCTCC TGACTGGAGC GAGGATTTTT TAGCCAATGT TTCAAGCAAG 6361 ATTTACGAGT CGATTCATCA GGTAGACTCA GCAGCCCAAT GGTTACAAAT GACCTGGATG 6421 TTCTTCTATG ACAAAAAGAA ATGGACTCAA CCCCGTATCC GTTCCTTTCT AAAAGCAGTA 6481 CCGGATAACA AACTGATATT ACTCGATTAC TATTGCGATC ATACAGAAAT CTGGCGAAAT 6541 ACTGAAAAAT ATTACGGGAA TCCCTATATT TGGTGTTATC TGGGTAATTT TGGCGGCAAT 6601 ACGATGATAG CAGGCAATCT GAATGATATT GATTTCAAGA TTAAACGACT CTTCAAGGAG 6661 GGAGGAGACA ATGTCTATGG CTTGGGAGCA ACTCTTGAAG GCTTTGATGT AAACCCTCTT 6721 ATGTACGAAT TTGTTTTTGA CCAGGCATGG GATTATCCTG TCACTACCGA CCAATGGATA 6781 ACCAACTGGT CCATGTGCAG AGGAGGAAAT CAGGATGCCA ATATCATCAA AGCGTGGAGA 6841 GCCTTGCATC AGAAAATTTA TACCGAACAT GCCACATGCG GCCAATCGGT ATTAATGAAC 6901 GCACGCCCCA GACTAACGGG AACTAAAAGT TGGAATACAA ACCCCGGCAT ACATTACGCA 6961 AATAACGACT TATGGCAAAT ATGGAAAGAG CTACTAAAAG CCCGGAACAT CAACAATAGT 7021 GATTTTCGCT TTGACGTAAT CAATATCGGA AGACAAGTAC TAGGAAACTT GTTCTCCGAA 7081 TATAGAGATC AGTTTACCGC TTGCTATAAC CGGAAAGATA CAACAGGCAT GAGAGAATGG 7141 AGTACCCGTA TGGACAACCT CTTGCTTGAC GTAGACCGCC TCCTTTCCTG TGACGCGACT 7201 CTATCCATCG GGAAATGGTT ACAAGACGCC AGAAACTGTG GTGCAACCGT GTCGGAAAAG 7261 GATTACTATG AAGAAAATGC ACGCTGTATC CTGACCGTAT GGGGGCAACA GGACACCCAA 7321 CTGAATGACT ATGCCAATCG AGGCTGGGGC GGACTTACCC GAAGCTTCTA CCGGGAACGT 7381 TGGAAACGTT TCACAGACGG AGTCATCGCT GCTGTTTCGG AAGATAAACC TTTTGACGAA 7441 GACAAATTCC ACCAGGACAT TACGCAATTT GAATACAACT GGACATTACA GAAAGACTCT 7501 TTCCCTATTG TTTCAGAGGA AGACCCGATT CAAATAGCAG ACAGTCTTAT CTTAAAATAC 7561 GATACGTACT TTACCAAAGC ACAATAGGAC CCAGCTTTCT TGTACAAAGT GGTTGATtcg 7621 aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc 7681 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 7741 gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat 7801 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat 7861 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc 7921 gatgataagc tgtcaaacat gagaa //