LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2756..2852) /label=attL2 /note="attL2 (c)" misc_signal complement(2867..2886) /note="T7 Promoter/priming site (c)" primer_bind 2894..2910 /note="M13 Reverse priming site" gene 3023..3832 /note="Kanamycin resistance gene" rep_origin 3953..4626 /note="pUC origin" vector join(2764..4629,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2749 /note="BGH89_B01c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGAGGTTTCT GATTCAGGAG ATGGAAAGGT 721 TTTAATAAAG GGGAATAATG GTGTTTCCTT AGCATCAGGA TTTAACTATT ATTTAAAAAA 781 TTATTGTAAT GTAAGTTACA ATCCAATAAT GGGGTCTAAT TTAAAAATGC CTGAAACAAT 841 GCCTTCTGTT GGGGAGAGAG TAGTTATAGA TACTCCATAT GAGCATAGAT ATGCATTAAA 901 CTTTTGTACC TATTCTTATA CAATGTCTTT CTGGGATTGG GATCAATATG AAGAATTCCT 961 AGATTGGTGT GCTATGAATG GGGTTAACTT AGTTTTAGAT ATAATTGGAC AAGAAGAGGT 1021 TTTAAGAAGA ACCTTAAATG AATTTGGATA TAGCGATGAA GAGGTTAAAG AATTTATTTC 1081 AGGACCTGCA TATTTTGCTT GGTTCTATAT GCAAAATATG ACTGGATTTG GAGGTCCACT 1141 TCCTAACGAT TGGTTTGAGC AAAGAGCAGA GCTTGGAAGA AAAATGCATG ATAGAATGCA 1201 AAGTTTTGGA ATAAATCCTG TTCTTCAAGG ATACTCAGGT ATGGTTCCAA GAGACTTTAA 1261 GGAAAAAAAT CAAGAAGCAC AAACTATTTC TCAAGGGGGA TGGTGTGGCT TTGATAGACC 1321 AGATATGCTT AAAACATATG TAAATGAAGG GGAGGCAGAT TATTTCCAAA AGGTTGCAGA 1381 TGTTTTCTAT GAAAAGCAAA AAGAAGTGTT TGGAGATGTA ACTAACTTCT ATGGAGTAGA 1441 TCCATTCCAT GAGGGAGGAA ATACTGGTGA TTTAGATAAT GGAAAGATAT ATGAAATAAT 1501 TCAAAATAAG ATGATAGAGC ATGATAATGA TGCAGTTTGG GTTATACAAA ACTGGCAAGG 1561 AAATCCTAGT AATAATAAGT TAGAAGGATT AACTAAAAAA GACCAAGCTA TGGTATTAGA 1621 TCTTTTCTCA GAGGTAAGTC CAGACTGGAA TAGATTAGAG GAAAGAGATT TACCATGGAT 1681 TTGGAATATG CTTCATAATT TTGGAGGAAG AATGGGAATG GATGCAGCAC CAGAAAAACT 1741 TGCAACAGAA ATTCCTAAAG CCTTAGCTAA TTCAGAGCAT ATGGTTGGTA TAGGTATTAC 1801 ACCAGAGGCT ATAAATACAA ATCCTTTAGC GTATGAACTT TTATTTGACA TGGCTTGGAC 1861 TAGAGATCAA ATAAACTTTA GAACATGGAC TGAAGATTAT ATTGAAAGAA GATATGGTAA 1921 GACTAATAAA GAGATTTTAG AGGCTTGGAA TATTATATTA GACACTGCTT ACAAAAAGAG 1981 AAATGATTAT TACCAAGGTG CTGCTGAGTC TATTATAAAT GCAAGACCAG GCTTTGGAAT 2041 AAAAAGTGCT TCAACTTGGG GACATTCAAA AATAGTTTAT GATAAGAGTG AATTTGAAAA 2101 AGCTATTGAA ATATTTGCAA AGAACTATGA TGAATTCAAA GATAGTGATG CATTCCTTTA 2161 TGACTTTGCT GATATATTAA AACAATTATT AGCTAACTCA GCTCAAGAAT ATTATGAGGT 2221 TATGTGTAAT GCTTATAATA ATGGAAATGG AGAGAAATTT AAATTTGTTT CAGGAAAGTT 2281 TTTAGAACTT ATAAAGCTTC AAGAGAGAGT ATTATCAACT AGACCTGAAT TCTTAATAGG 2341 AAATTGGATT GAAGATGCTA GAACTATGCT TAAGGACTCT GATGATTGGA CTAAGGACTT 2401 ATTTGAATTT AATGCTAGAG CTTTAGTAAC AACTTGGGGT TCAAGAAATA ATGCTGACGG 2461 TGGTGGGCTT AAAGATTATT CTAATAGACA ATGGTCAGGA TTAACTGAGG ATTATTATTA 2521 TGCTAGATGG GAAAAATGGA TAAATGGATT ACAAGCTGAA TTAGATGGAG GAGCTAAAGC 2581 CCCTAATATA GATTGGTTTA AGATGGAGTA TGACTGGGTA AACAAGAAGA GTGATACAGA 2641 TAAGCTTTAT CCTACAGAAG CTTCTAATGA AAACTTAGGA GAACTTGCTA AGATAGCCAT 2701 GGAAAGTTAT TCAGTAACTA ATATGGATAA AATATTAGGT GAAAATGAAT AGGACCCAGC 2761 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2821 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2881 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2941 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 3001 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 3061 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 3121 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 3181 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 3241 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 3301 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 3361 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 3421 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 3481 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 3541 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 3601 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 3661 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3721 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3781 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3841 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3901 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3961 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 4021 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 4081 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 4141 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 4201 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 4261 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 4321 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 4381 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 4441 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 4501 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 4561 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4621 TCACATGTT //