LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1472..1568)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1583..1602)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1610..1626
                     /note="M13 Reverse priming site"
     gene            1739..2548
                     /note="Kanamycin resistance gene"
     rep_origin      2669..3342
                     /note="pUC origin"
     vector          join(1480..3345,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..1465
                     /note="BGT02_T01a"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cACAAATGAT CATAGAATGC TGTCGGTTGT
        721 GGTTCCTGCG TACAACGAAG AAAAACGTTT GTCCGCATCT TTAGAGGTTC TTTGTGAAAA
        781 GGTCGGGCTG TTTTTCCCTC GTTTTGAAAT TATTGTCGTG GATGATGGTA GTACCGATAA
        841 AACAGCGGAT ATCGTTATGA CCCATAGCCG AAAATATTCG GATGTCCGCC TTATTCGTTA
        901 TGAAAAAAAC CGGGGCAAAG GCTATGCTGT TCGTACCGGT GTATTGGCTG CAAAGGGCGA
        961 TTTCGTGTTG TTCAGCGATG CCGACTTATC AACACCTATC GAAGAGGTTG AGAAGTTGTT
       1021 TGGTGCACTC GCGGATGGAG CCGATGTCGC TATAGGGTCG CGGGCTGTGC GCCAATCGCT
       1081 GATTCTAAAG AGTCAGCCTC TTTACCGTAT GGTGATGGGG AAAACTTTTA ATAAATTTGT
       1141 ACAGCTGCTG GCGATCCCCG GTATTTTAGA TACGCAATGC GGATTTAAGC TTTTCACGCG
       1201 TTCTGCCGCT TTAAATCTTT TCAGAGATTG TCGCATTGAT GGTTTTGGTT TCGATGTGGA
       1261 GGTTCTTTTT CTTGCGCGTA AAAGAGGCAT GGATATACGC GAGATTGGCG TAAGCTGGGT
       1321 CAATTCACCC GATAGCAAAG TACATCCCAT CGTTGATTCG GCACGTATGC TCCAAGATTT
       1381 GGTTGTTATT CGCAGACATG CGTTGTTGGG GGATTACGGG GATTTAAGAT TGCCACAAGC
       1441 TAAAATCGAA ACAGTATCTC CCGATTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT
       1501 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
       1561 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT
       1621 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT
       1681 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT
       1741 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
       1801 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT
       1861 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
       1921 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
       1981 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
       2041 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
       2101 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
       2161 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
       2221 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
       2281 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
       2341 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
       2401 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
       2461 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
       2521 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA
       2581 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG
       2641 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
       2701 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
       2761 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
       2821 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
       2881 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
       2941 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
       3001 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
       3061 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
       3121 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
       3181 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
       3241 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
       3301 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//