LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1601..1697)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1712..1731)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1739..1755
                     /note="M13 Reverse priming site"
     gene            1868..2677
                     /note="Kanamycin resistance gene"
     rep_origin      2798..3471
                     /note="pUC origin"
     vector          join(1609..3474,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..1594
                     /note="BGT02_T03b"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cAACGACGGC GACGGGACGC CCGCGGCTGC
        721 GGCGCGGGAG AGGCGGTTCG GCCCGCTGGG CACGGCCCTG GTGATCATCC CGACCTACAA
        781 CGAGGCGGAG AACATCAAGG CGATCGTCAC GCGTGTGCGC GAGGCCGTCC CCGAGGCACA
        841 CGTGCTCGTG GCCGACGACA ACAGCCCCGA CGGCACCGGC AAGCTCGCCG ACGAACTGGC
        901 GGCCGCGGAC GACCACGTCC AGGTCATGCA CCGCAAGGGC AAGGAAGGGC TGGGCGCCGC
        961 CTACCTCGCC GGCTTCCGCT GGGGCCTGGA GCACGGCTAC GGCGTGCTGA TCGAGATGGA
       1021 CGCCGACGGC TCCCACCAGC CCGAGGAACT GCCCCGTCTG CTGACCGCCC TCAAGGGCGC
       1081 CGACCTCGTC CTCGGCTCGC GCTGGGTGCC CGGCGGCCGG GTGGTCAACT GGCCCAAGTC
       1141 CCGCGAGATC ATCTCGCGTG GCGGCAGCAT GTACTCGAGA ATCGCGCTCG ACCTGCCGCT
       1201 GCGCGACATC ACCGGCGGCT ACCGCGCCTT CCGCCGCGAG ACCCTGGAGG GGCTCGGCCT
       1261 CGAGGAGGTC GCCTCCCAGG GGTACTGCTT CCAGGTCGAC CTCGCCCGCC GGGCGATCAA
       1321 GGCCGGCTAT CACGTCGTGG AGGTGCCGAT CACGTTCGTG GAGCGGGAAC TCGGCGACTC
       1381 CAAGATGAGC CGCGACATCC TGGTGGAGGC CCTGTGGCGG GTCACCACGT GGGGCGTGGG
       1441 GGAGAGGGTC GGCAAGGTGC TCGGCCGCGA CAGGCGGTCC GGCACGGCGG CCGACGGGCA
       1501 GACGGCCTCG AGCGGGACCG ACGGGCGGAC GGCCTCGAGC GACAGGTCGA CGGACTCCCT
       1561 CGACGAGTCG GCGAAGGCGT CCGACGCCAA GGCCTAGGAC CCAGCTTTCT TGTACAAAGT
       1621 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA
       1681 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT
       1741 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA
       1801 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG
       1861 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA
       1921 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT
       1981 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG
       2041 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC
       2101 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC
       2161 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT
       2221 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC
       2281 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT
       2341 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA
       2401 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC
       2461 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT
       2521 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC
       2581 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA
       2641 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA
       2701 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT
       2761 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
       2821 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
       2881 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
       2941 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
       3001 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
       3061 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
       3121 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
       3181 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
       3241 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
       3301 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
       3361 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
       3421 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT
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