LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1886..1982) /label=attL2 /note="attL2 (c)" misc_signal complement(1997..2016) /note="T7 Promoter/priming site (c)" primer_bind 2024..2040 /note="M13 Reverse priming site" gene 2153..2962 /note="Kanamycin resistance gene" rep_origin 3083..3756 /note="pUC origin" vector join(1894..3759,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..1879 /note="BGT02_T05a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cTTAAACTTT TTAAGTATCT TTTTATTGAC 721 TTATGGTGTG CTTGCTATTT CGCACATTGC ATTCCAAATT ATTCTATGTC ATAGTGACCA 781 TAGACGACAG AGCAAGAAAT CATTTAAGGA TTTCCATAGT AACTATCAGG CTAGTGTTTC 841 AGTCATTGTG CCTGCATATA ATGAAGAACC ACAAATTTTG AAGAATTGTA TTGATTCTAT 901 TGTGGCACAA AAAGCACCTG ACCTAGAGAT TATTGTTGTA GATGACGGAT CAAAAAATCG 961 TGAAGAATTG ATTGAAAAAG TCTATAACAC TTATCAATCA AATCAAAATG TTAAAATTTT 1021 ATTGCCTGAG GAAAACAAAG GCAAACGTCA CTGTCAAAAA CTTGGATTTG ATATTGCTAA 1081 GGGCGATATT ATTGTCACAG TGGATTCAGA TACCTTACTA CATGATGAGA ATGCAGTTGA 1141 AAAATTAATT CAGCGTTTTG CTTATAAGAA CGTTGGGGCT GTCACTGGTG ATGTTCGAGT 1201 CGAAAACAAA AATACGAATA TCTTAACACG TCTGATTACG TATCGCTATT GGACTGCGTT 1261 TCACCAAGAG CGAGCAGCTC AAAGCCGCTT TCACGTTGTA ATGTGTTGTT CTGGACCGTT 1321 TTCAGCCTAT CGTAAAGAAA TAATTGACAA AGTCAAAGAG AAATACATTA CACAATATTT 1381 CTTAGGCGAA AATTGTACCT ATGGAGATGA CCGTCATTTG ACAAATTTGG TTCTTGAAGA 1441 AGGCCATGAC GTTGCCTTCC ACAGAGATAG TAGAGTTTAT ACTTTTGTAC CTGAAACAAT 1501 TCGTGGATAT ATCAAACAGC AAGTACGATG GAATAAAAGC TTCTATCGTG AGATGCTTTG 1561 GACAATTAAG TTTGCACCTA AACGTCATTT TTATATGCTT TATGATTTGG TCATGCAATT 1621 TATCTTACCG TTTATGTTGG TTGTATCATT AATTGCTATG GCTGTCCAAA CAATTTCATA 1681 TCATGATTTA GGACATTTCT ATCATTATTT GCTTGTTTTG ATTCTGATCG CTATTTTCCG 1741 TTCACTGTAT GGTATTTATC GAACTAAAGA CATTGGATTT TTACTCTTTG TTTTATATGG 1801 CTTTATGCAC GTACTAATTC TATTACCTGT TCGATTCTAT GCACTATTCA CATTGAAATC 1861 GACAAAATGG GGAACGCGAT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 1921 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 1981 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2041 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2101 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2161 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2221 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2281 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2341 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2401 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2461 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2521 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2581 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2641 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 2701 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 2761 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 2821 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 2881 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 2941 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3001 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3061 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3121 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3181 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3241 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3301 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3361 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3421 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3481 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3541 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3601 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3661 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3721 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //