LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1514..1610)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1625..1644)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1652..1668
                     /note="M13 Reverse priming site"
     gene            1781..2590
                     /note="Kanamycin resistance gene"
     rep_origin      2711..3384
                     /note="pUC origin"
     vector          join(1522..3387,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    671..691
                     /note="TEV site"
     misc_feature    692..1507
                     /note="BGT25_T02c"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cCGTGTTTTT ATCATTTCTT TAAATCAAAA
        721 AGTGTGCGAT AAATTTGGTT TGGTTTTTAG AGACACCACA ACTTTACTCA ATAATATCAA
        781 CGCCACCCAC CACCAAGCGC AAATTTTTGA TGCGATTTAT TCCAAGACTT TTGAAGGCGG
        841 GTTGCACCCC CTAGTTAAAA AGCATTTACA CCCTTATTTC ATCACGCAAA ATATCAAAGA
        901 CATGGGGATT GCAACCAGTC TCATCAGTGA GGTTTCTAAG TTTTATTACG CTTTAAAATA
        961 CCATGCGAAA TTTATGAGCT TGGGGGAGCT TGGGTGCTAT GCGAGCCATT ATTCGTTGTG
       1021 GCAAAAATGC ATAGAGCTCA ATGAAGCGAT CTGTATTTTA GAAGACGATA TAACCTTGAA
       1081 AGAGGATTTT AAAGAGGGCT TGGATTTTTT AGAAAAACAC ATCCAAGAGT TAGGCTATGC
       1141 GCGTTTGATG CATTTATTGT ATGATGCCAG CGTGAAAAGT GAGCCTTTAA ACCATAAAAA
       1201 CCAAGAGATA CAAGAGCGTG TGGGGATCAT TAAAGCTTAT AGCCATGGGG TGGGGACGCA
       1261 AGGCTATGTG ATCACGCCCA AGATTGCCAA AGTTTTTTTG AAACACAGCC GAAAATGGGT
       1321 TGTTCCTGTG GATACGATAA TGGACGCTAC TTTTATCCAT GGCGTGAAAA ATCTGGTGTT
       1381 ACAACCTTTT GTGATCGCTG ATGATGAGCA AATCTCTACG ATAGCGCGAA AAGAAGAACC
       1441 CTATAGCCCT AAAATCGCTC TAATGAGAGA ACTCCATTTT AAATTTTTGA AATGGTGGCA
       1501 GTTTGTATAG GACCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC
       1561 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC
       1621 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC
       1681 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT
       1741 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA
       1801 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC
       1861 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC
       1921 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT
       1981 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG
       2041 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT
       2101 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG
       2161 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT
       2221 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA
       2281 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC
       2341 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG
       2401 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT
       2461 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA
       2521 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA
       2581 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG
       2641 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG
       2701 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
       2761 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
       2821 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
       2881 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
       2941 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
       3001 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
       3061 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
       3121 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
       3181 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
       3241 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
       3301 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
       3361 CTTTTGCTGG CCTTTTGCTC ACATGTT
//