LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2507..2603) /label=attL2 /note="attL2 (c)" misc_signal complement(2618..2637) /note="T7 Promoter/priming site (c)" primer_bind 2645..2661 /note="M13 Reverse priming site" gene 2774..3583 /note="Kanamycin resistance gene" rep_origin 3704..4377 /note="pUC origin" vector join(2515..4380,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2500 /note="BGT03_T01a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cAACACTAAT TATTCTGACT GTTTATTAAC 721 CGAAGTAGCC TGGGAAGTAT GTAATCAGGT AGGTGGTATC TATACCGTTA TCCGTTCAAA 781 AGTACCTACC ATGACTGAAG TATGGGGCGA TCGCTACTGT TTGGTTGGAC CGTATTTTCA 841 CAACAAAGCC ATTGCTGAAT TTGAAGCGAT TGATACCTAC GAAGCTGATG ATGTTTTTGC 901 ACAGGCTGTA TTGAGAATGC GCAACTTAGG TTATGAGGTT CATTACGGAC ACTGGCTGGT 961 ATCAGGCCGT CCGAAAGTTG TATTGATAAA TCATTTCTCT GCATTCTACC GGTTAGGTGA 1021 AATTAAATAC AGCCTTTGGG AAAACCACGG AATCTCAAGT CCGGAAGGGG ATGACCTGTT 1081 AAATCAGGTA ATTGCTTTTG GTTTCCTTGC AAAGGTGTTC CATCAGCAAT TGGATGAAAC 1141 AAAAGGAGAA CTTCCATGTG TATCTCATTT CCACGAATGG ATGACAGGTA CAGCGATTCC 1201 TGAAATGCGC CGCGAACATG TAAAAACATC TATTGTATTT ACAACCCACG CCACCTTATT 1261 GGGCCGCTAC CTGGCCATGA ATGATCCGAA TTTCTATGAC AACTTACCTT ATTACGATTG 1321 GAATAAAGAA GCGGTCAATT TTAATATCGA ACCTGCAGTA AAAATAGAAC GCGCTGCCGC 1381 ACACGGCGCG AACGTATTTA CAACCGTAAG TGAAGTAACA GCCCGTGAAT GCCAGTCGTT 1441 ACTGGGCAGA AACCCGGACA TGGTTTTGCC CAACGGTTTG AACATTGAAC GTTTCACTGC 1501 CTTACACGAA TTCCAGAATT TACACAAAGA ACATAAAGAT CAGATCCATG AATTTGTGAT 1561 CGGTCACTTT TTTCAAAGCT ATACGTTTGA CCTGGATAAG ACCTTATACT TCTTTACTTC 1621 CGGAAGATAT GAGTACAAGA ATAAAGGCTT CGACATTACG CTTGAAGCGC TTGCCCGTTT 1681 AAACTGGAGA TTAAAAGAAG AGAACACCGA TACAACGGTT GTGATGTTCT TTATTACCAA 1741 GCAGCCATAC CATACCATTA ACCCGCAGGT ATTACAATCA CGCGCGGTAA TGGAAGAGGT 1801 GAGAAGCAAC TGCGATGCAA TTGTACAGCA GCTGGGCGAT AAATTGTTTG AAGCGGCTGC 1861 ATCCAGCGGC GACTTAAAAT TACCGGATTT AAATAAATTT GTAGACGAAT ACTGGAAGCT 1921 GCGTTTAAGA AGAACCTTGC TATCCTGGAA GTCGCATGAA TTGCCAAAGG TTGTTACACA 1981 CAATTTAGTG TACGACAATC AGGATGAAAT TCTGAACTTC CTGAGACATT CCAATATGGT 2041 AAACAATGAA CACGATAAAG TAAAAGTTGT TTATCACCCG GACTTCATTG CAAGCACCAA 2101 TCCCTTGTTT GGCATGGAAT ACGGACAGTT TGTACGCGGT TGCCATTTAG GTGTGTTCCC 2161 AAGTTACTAC GAACCATGGG GCTATACGCC GCTTGAATGT ATGGCATCCG GTGTGCCTGC 2221 AATCACATCC GATTTATCCG GATTCGGTGA CTACGTATTG AAAAACATTC CGAATAATGA 2281 GAACAAAGGT ATCTACGTAA CAAACCGCTT CCATAAGAGT TACCACGATG CTGCACAGCA 2341 GCTGGCAGAC CAGATGTATC ACTTTGTTCA CCTCTCCCGC AGAGACCGTA TAACGCAACG 2401 TAACAGAGTG GAGAGTGCAT CCGAACATTT TGACTGGCAG AACTTAGGAA GCTATTATGA 2461 TAAAGCGCAT CGTTTGGCTT TCTCTATTGC AGTGAACGCT TAGGACCCAG CTTTCTTGTA 2521 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2581 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2641 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2701 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2761 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2821 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2881 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2941 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 3001 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3061 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 3121 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 3181 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3241 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3301 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3361 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3421 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3481 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3541 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3601 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3661 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3721 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3781 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3841 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3901 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3961 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4021 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4081 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 4141 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4201 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4261 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4321 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //