LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2354..2450) /label=attL2 /note="attL2 (c)" misc_signal complement(2465..2484) /note="T7 Promoter/priming site (c)" primer_bind 2492..2508 /note="M13 Reverse priming site" gene 2621..3430 /note="Kanamycin resistance gene" rep_origin 3551..4224 /note="pUC origin" vector join(2362..4227,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 671..691 /note="TEV site" misc_feature 692..2347 /note="BGT03_T01c" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cGTAAAAGAT TTATTAACCC CCGATTATAT 721 CTTCGAGTCC AGCTGGGAAG TATGTAATAA AGTGGGAGGG ATATACACCG TATTGTCGAC 781 ACGGGCGAAT ACATTGCAGG AAAAATTCCG CGACAGAATT TTTTTCATAG GTCCTGATGT 841 GTGGCAAGGA AAAGAGAACC CTCTATTCAT CGAGTCGGAT AATCTGTGCG CTGACTGGAA 901 AAAGCACGCA TTCAAAAACG ATCATCTTTC CGTCCGTGTA GGACGATGGA ATATACCCGG 961 TGAACCCATT GTCATCCTTG TTGATTTCCA ACCTTTTTTT GAACAGAAGA ACGAAATTTA 1021 CACAGAAATG TGGAATCGTT TTCAAGTAGA CTCGTTGCAC GCTTATGGCG ATTACGACGA 1081 GGCTTCGATG TTTTCGTATG CTGCCGGAAA AGTAGTAGAG AGTTTCTACC GCTACAACCT 1141 GACAGAAACG GATAAGGTGG TATATCAGGC ACATGAATGG ATGACCGGAA TGGGAGCACT 1201 TTATGTACAG GAAGCTGTCC CCGAAGTGGC TACTATTTTT ACAACCCACG CTACTTCCAT 1261 CGGACGTTCG ATAGCCGGCA ATCATAAGCC GCTGTATGAC TATCTGTTTG CTTATAACGG 1321 TGACCAGATG GCGCAGGAAC TGAATATGCA GTCAAAGCAC TCTATTGAGA AGCAGACGGC 1381 GCATTATGTA GACTGTTTTA CGACAGTAAG TGAAATCACG AATAACGAGT GCAAGGAATT 1441 GCTCGACAAA CCGGCAGACG TAGTACTGAT GAACGGTTTC GAAGACGATT TCGTACCTAA 1501 AGGCAGTACA TTTACCGGAA AACGTAAACG TGCCCGCTCA TTGATGTTGA ACGTAGCCAA 1561 TAAATTGTTG GGAACAAATC TGGATGACGA TACGTTAATT ATCGGTACCA GTGGTCGATA 1621 TGAATTCAAG AACAAGGGTA TTGACGTATT CCTGGAGTCA TTGAACCGTC TGAACAGGGA 1681 TAAAAAGCTC CATAAGAACG TATTGGCGTT TATCAATGTC CCCGGTTGGG TAGGAGATCC 1741 CCGTGAAGAC TTGCAGGAAC GTCTGAAAAG CAAAGAAAAA TTTGATACAC CGCTTGAAGT 1801 TCCTTTCATC ACTCATTGGC TGCACAATAT GACGCATGAC CAAGTGCTGG ATATGCTGAA 1861 ATATCTGGGG ATGGGCAACC GTCCCGATGA TAAAGTAAAG GTGATTTTTG TGCCTTGCTA 1921 TCTGGACGGG CGTGATGGTA TCCTGAATAA AGAGTATTAT GATATATTGC TGGGACAGGA 1981 TTTGAGTGTA TATGCTTCCT ATTACGAACC GTGGGGATAT ACTCCGCTGG AAAGTGTAGC 2041 GTTCCGTGTG CCTACCATTA CTACTGATCT GGCAGGTTTT GGCCTTTGGG TGAACAGCCT 2101 GAAGAATCAG CATGGCATCA ATGATGGAGT GGAAGTGCTG CATCGTTCGG ACTATAATGA 2161 TTCGGAAGTG GCGGATGGCA TCAAAGATAC GATTACGCTG TTTGCAGATA AGTCGGAAAA 2221 AGAAGTGAAG GAGATCCGTA AGCATGCGGC AGATGTTGCA GAGCAGGCAT TGTGGAAACA 2281 CTTCATTCAA TATTATTACG AGGCTTATGA TATTGCCTTG CGCAATGCCA TGAAGCGTCA 2341 GTTAGGCTAG GACCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 2401 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 2461 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 2521 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2581 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2641 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2701 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2761 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2821 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2881 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2941 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 3001 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 3061 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 3121 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 3181 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 3241 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 3301 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 3361 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 3421 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 3481 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3541 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3601 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3661 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3721 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3781 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3841 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3901 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3961 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 4021 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 4081 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 4141 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 4201 CTTTTGCTGG CCTTTTGCTC ACATGTT //