LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2102..2198) /label=attL2 /note="attL2 (c)" misc_signal complement(2213..2232) /note="T7 Promoter/priming site (c)" primer_bind 2240..2256 /note="M13 Reverse priming site" gene 2369..3178 /note="Kanamycin resistance gene" rep_origin 3299..3972 /note="pUC origin" PCR_frame 669..2101 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2121) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2118) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2110..3975,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2080..2100 /note="TEV site" source 673..2079 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:126878 IMAGE:8069335" /tissue_type="Liver, PCR rescued clones" /clone_lib="NIH_MGC_291" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: ATATTAAT" gene 673..2079 /gene="GBA3" /gene_synonym=CBGL1 /gene_synonym=GLUC /db_xref="GeneID:57733" /db_xref="HGNC:19069" /db_xref="IMGT/GENE-DB:19069" /db_xref="MIM:606619" CDS 673..2079 /dnas_title="cytosolic beta-glucosidase" /gene="GBA3" /gene_synonym=CBGL1 /gene_synonym=GLUC /codon_start=1 /product="cytosolic beta-glucosidase" /protein_id="AAI01830.1" /db_xref="GI:75517945" /db_xref="GeneID:57733" /db_xref="HGNC:19069" /db_xref="IMGT/GENE-DB:19069" /db_xref="MIM:606619" /translation="MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVF KNQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNK IIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIESFDKYAQFCFSTFGDRVKQW ITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWHSYDSLFRKKQKG MVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASMS QKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEF FPDPSWKNVDWIYVVPWGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEY FRQTFQELFKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPY TSAKEYAKIIRNNGLEAHL" misc_difference 2040..2040 /gene="GBA3" /gene_synonym=CBGL1 /gene_synonym=GLUC /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'Y' in cDNA, '*' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 673..2100 /note="GBA3 coding region" CDS 673..2100 /note="GBA3 coding region" /translation="MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVF KNQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNK IIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIESFDKYAQFCFSTFGDRVKQW ITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWHSYDSLFRKKQKG MVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASMS QKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEF FPDPSWKNVDWIYVVPWGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEY FRQTFQELFKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPY TSAKEYAKIIRNNGLEAHLENLYFQG" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcttt ccctgcagga tttggatggg cggcagccac tgcagcttat 721 caagtagaag gaggctggga tgcagatgga aaaggccctt gtgtctggga cacatttact 781 catcagggag gagagagagt tttcaagaac cagactggcg atgtagcttg tggcagctac 841 actctgtggg aggaagattt gaaatgtatc aaacagcttg gattgactca ttaccgcttc 901 tctctttcct ggtcacgtct gttacctgat gggacgacag gtttcatcaa ccagaaagga 961 attgattatt acaacaagat catcgatgat ttgttaaaaa atggggttac tcccattgtg 1021 accctctacc actttgattt gcctcagact ttagaagacc aaggaggttg gttgtcagag 1081 gcaatcattg aatcctttga caaatatgct cagttttgct tcagtacctt tggggatcgt 1141 gtcaagcagt ggatcaccat aaatgaagct aatgttcttt ctgtgatgtc atatgactta 1201 ggtatgtttc ctccgggtat ccctcacttt gggactggag gttatcaggc agctcataat 1261 ttgattaagg ctcatgccag atcctggcac agctatgatt ccttatttcg aaaaaagcag 1321 aaaggtatgg tgtctctatc actttttgcg gtctggttgg aaccagcaga tcccaactca 1381 gtgtctgacc aggaagctgc taaaagagcc atcactttcc atctggattt atttgctaaa 1441 cccatattca tcgatggtga ttatcctgaa gttgtcaagt ctcagattgc ctccatgagt 1501 caaaagcaag gctatccatc atcgaggctt ccagaattca ctgaagaaga gaagaaaatg 1561 atcaaaggca ctgctgattt ttttgctgtg caatattata caactcgctt aatcaagtac 1621 caggagaaca agaaaggaga actaggtatt ctccaggatg cggaaattga attttttcca 1681 gatccatctt ggaaaaatgt ggattggatc tacgtggtac catggggagt atgtaaacta 1741 ctgaaatata ttaaggatac atataataac cctgtaattt acatcactga gaatgggttt 1801 ccccagagtg acccagcgcc tcttgatgac actcaacgct gggagtattt cagacaaaca 1861 tttcaggaac tgttcaaagc tatccaactt gataaagtca atcttcaagt atattgtgca 1921 tggtctcttc tggataactt tgagtggaac cagggataca gcagccggtt tggtctcttc 1981 cacgttgatt ttgaagaccc agctagaccc cgagtccctt acacatcggc caaggaatat 2041 gccaagatca tccgaaacaa tggccttgaa gcacatctgg aaaacttgta ctttcaaggc 2101 tCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2161 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2221 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2281 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2341 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2401 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2461 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2521 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2581 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2641 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2701 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2761 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2821 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2881 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2941 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3001 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3061 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3121 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3181 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3241 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3301 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3361 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3421 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3481 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3541 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3601 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3661 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3721 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3781 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3841 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3901 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3961 TTTTGCTCAC ATGTT //