LOCUS GBA3-pGEc2-DEST 6628 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6106..6235) /note="3'-ITR" misc_feature complement(5804..6018) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5256..5797 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4435..4457 /note="attB2" misc_feature 5235..5240 /note="BstB1 site" misc_feature 4458..5153 /note="Super GFP" misc_feature 5154..5204 /note="AviTag" misc_feature 5205..5228 /note="His Tag" PCR_frame 3002..4434 /note="Reading Frame" /set="Set 1" source 3006..4412 /dnas_title="GBA3-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:126878 IMAGE:8069335" /tissue_type="Liver, PCR rescued clones" /clone_lib="NIH_MGC_291" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: ATATTAAT" gene 3006..4412 /dnas_title="GBA3" /gene="GBA3" /gene_synonym="CBGL1" /gene_synonym="GLUC" /db_xref="GeneID:57733" /db_xref="HGNC:19069" /db_xref="IMGT/GENE-DB:19069" /db_xref="MIM:606619" CDS 3006..4412 /dnas_title="cytosolic beta-glucosidase" /gene="GBA3" /gene_synonym="CBGL1" /gene_synonym="GLUC" /codon_start=1 /product="cytosolic beta-glucosidase" /protein_id="AAI01830.1" /db_xref="GI:75517945" /db_xref="GeneID:57733" /db_xref="HGNC:19069" /db_xref="IMGT/GENE-DB:19069" /db_xref="MIM:606619" /translation="MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVF KNQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNK IIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIESFDKYAQFCFSTFGDRVKQW ITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWHSYDSLFRKKQKG MVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASMS QKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEF FPDPSWKNVDWIYVVPWGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEY FRQTFQELFKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPY TSAKEYAKIIRNNGLEAHL" misc_feature 3006..4433 /note="GBA3 coding region" CDS 3006..4433 /note="GBA3 coding region" /translation="MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVF KNQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNK IIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIESFDKYAQFCFSTFGDRVKQW ITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWHSYDSLFRKKQKG MVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASMS QKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEF FPDPSWKNVDWIYVVPWGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEY FRQTFQELFKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPY TSAKEYAKIIRNNGLEAHLENLYFQG" misc_feature 4413..4433 /note="TEV site" misc_difference 4373..4373 /dnas_title="GBA3" /gene="GBA3" /gene_synonym="CBGL1" /gene_synonym="GLUC" /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'Y' in cDNA, '*' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4442) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4442) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4443..6628,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4442 /source="GBA3-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc tttccctgca ggatttggat gggcggcagc cactgcagct tatcaagtag 3061 aaggaggctg ggatgcagat ggaaaaggcc cttgtgtctg ggacacattt actcatcagg 3121 gaggagagag agttttcaag aaccagactg gcgatgtagc ttgtggcagc tacactctgt 3181 gggaggaaga tttgaaatgt atcaaacagc ttggattgac tcattaccgc ttctctcttt 3241 cctggtcacg tctgttacct gatgggacga caggtttcat caaccagaaa ggaattgatt 3301 attacaacaa gatcatcgat gatttgttaa aaaatggggt tactcccatt gtgaccctct 3361 accactttga tttgcctcag actttagaag accaaggagg ttggttgtca gaggcaatca 3421 ttgaatcctt tgacaaatat gctcagtttt gcttcagtac ctttggggat cgtgtcaagc 3481 agtggatcac cataaatgaa gctaatgttc tttctgtgat gtcatatgac ttaggtatgt 3541 ttcctccggg tatccctcac tttgggactg gaggttatca ggcagctcat aatttgatta 3601 aggctcatgc cagatcctgg cacagctatg attccttatt tcgaaaaaag cagaaaggta 3661 tggtgtctct atcacttttt gcggtctggt tggaaccagc agatcccaac tcagtgtctg 3721 accaggaagc tgctaaaaga gccatcactt tccatctgga tttatttgct aaacccatat 3781 tcatcgatgg tgattatcct gaagttgtca agtctcagat tgcctccatg agtcaaaagc 3841 aaggctatcc atcatcgagg cttccagaat tcactgaaga agagaagaaa atgatcaaag 3901 gcactgctga tttttttgct gtgcaatatt atacaactcg cttaatcaag taccaggaga 3961 acaagaaagg agaactaggt attctccagg atgcggaaat tgaatttttt ccagatccat 4021 cttggaaaaa tgtggattgg atctacgtgg taccatgggg agtatgtaaa ctactgaaat 4081 atattaagga tacatataat aaccctgtaa tttacatcac tgagaatggg tttccccaga 4141 gtgacccagc gcctcttgat gacactcaac gctgggagta tttcagacaa acatttcagg 4201 aactgttcaa agctatccaa cttgataaag tcaatcttca agtatattgt gcatggtctc 4261 ttctggataa ctttgagtgg aaccagggat acagcagccg gtttggtctc ttccacgttg 4321 attttgaaga cccagctaga ccccgagtcc cttacacatc ggccaaggaa tatgccaaga 4381 tcatccgaaa caatggcctt gaagcacatc tggaaaactt gtactttcaa ggctCCAGCT 4441 TTCTTGTACA AAGTGGTATG AGCAAGGGCG AGGAACTGTT CACCGGCGTG GTGCCCATCC 4501 TGGTGGAACT GGATGGCGAC GTGAACGGCC ACAAGTTCTC TGTGCGGGGA GAGGGCGAAG 4561 GCGACGCCAC AAATGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG 4621 TGCCTTGGCC TACCCTCGTG ACCACACTGA CCTACGGCGT GCAGTGCTTC AGCAGATACC 4681 CCGACCATAT GAAGCGGCAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG 4741 AACGGACCAT CAGCTTCAAG GACGACGGCA CCTACAAGAC CAGAGCCGAA GTGAAGTTCG 4801 AGGGCGACAC CCTCGTGAAC CGGATCGAGC TGAAGGGCAT CGATTTCAAA GAGGACGGCA 4861 ACATCCTGGG CCACAAGCTG GAGTACAACT TCAACAGCCA CAACGTGTAC ATCACCGCCG 4921 ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG GCACAACGTG GAAGATGGCA 4981 GCGTGCAGCT GGCCGACCAC TACCAGCAGA ACACCCCCAT CGGAGATGGC CCCGTGCTGC 5041 TGCCCGACAA CCACTACCTG AGCACCCAGA GCGTGCTGAG CAAGGACCCC AACGAGAAGC 5101 GGGACCACAT GGTGCTGCTG GAATTCGTGA CCGCCGCTGG CATCACCCAC GGCATGAGCG 5161 GCCTGAACGA CATCTTCGAG GCCCAGAAAA TCGAGTGGCA CGAGCACCAC CATCATCACC 5221 ATCACCACTG ATGATTCGAA AAATAATAAG GATCCAATCA ACCTCTGGAT TACAAAATTT 5281 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 5341 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 5401 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 5461 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 5521 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 5581 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 5641 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 5701 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 5761 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC 5821 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 5881 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 5941 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 6001 TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC GATTAGGATC TTCCTAGAGC 6061 ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA 6121 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG 6181 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA 6241 ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC GCCAATTCGC CCTATAGTGA 6301 GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 6361 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 6421 AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAG 6481 CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 6541 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 6601 TAACAAAATA TTAACGCTTA CAATTTAG //