LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3638..3734) /label=attL2 /note="attL2 (c)" misc_signal complement(3749..3768) /note="T7 Promoter/priming site (c)" primer_bind 3776..3792 /note="M13 Reverse priming site" gene 3905..4714 /note="Kanamycin resistance gene" rep_origin 4835..5508 /note="pUC origin" vector join(3646..5511,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3631 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /tissue_type="kidney" gene 692..3631 /gene="klotho" CDS 692..3631 /gene="klotho" /codon_start=1 /protein_id="BAA23382.1" /db_xref="GI:2618596" /translation="MPASAPPRRPRPPPPSLSLLLVLLGLGGRRLRAEPGDGAQTWAR VSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPG DSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNG SAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRD YAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHA KVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFID GDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESP NLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGV DVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPP LPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTK KRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMAS ELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVK LWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACP FSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQ GTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVL NWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFA YSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTE CSFFHTRKSLLAFIAFLFFASIISLSLIFYYSKKGRRSYK" misc_feature 692..3631 /note="KL coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgcggagccg ggcgacggcg cgcagacctg 721 ggcccgtgtc tcgcggcctc ctgcccccga ggccgcgggc ctcttccagg gcaccttccc 781 cgacggcttc ctctgggccg tgggcagcgc cgcctaccag accgagggcg gctggcagca 841 gcacggcaag ggtgcgtcca tctgggacac gttcacccac caccccctgg cacccccggg 901 agactcccgg aacgccagtc tgccgttggg cgccccgtcg ccgctgcagc ccgccaccgg 961 ggacgtagcc agcgacagct acaacaacgt cttccgcgac acggaggcgc tgcgcgagct 1021 cggggtcact cactaccgct tctccatctc gtgggcgcga gtgctcccca atggcagcgc 1081 gggcgtcccc aaccgcgagg ggctgcgcta ctaccggcgc ctgctggagc ggctgcggga 1141 gctgggcgtg cagcccgtgg tcaccctgta ccactgggac ctgccccagc gcctgcagga 1201 cgcctacggc ggctgggcca accgcgccct ggccgaccac ttcagggatt acgcggagct 1261 ctgcttccgc cacttcggcg gtcaggtcaa gtactggatc accatcgaca acccctacgt 1321 ggtggcctgg cacggctacg ccaccgggcg cctggccccc ggcatccggg gcagcccgcg 1381 gctcgggtac ctggtggcgc acaacctcct cctggctcat gccaaagtct ggcatctcta 1441 caatacttct ttccgtccca ctcagggagg tcaggtgtcc attgccctaa gctctcactg 1501 gatcaatcct cgaagaatga ccgaccacag catcaaagaa tgtcaaaaat ctctggactt 1561 tgtactaggt tggtttgcca aacccgtatt tattgatggt gactatcccg agagcatgaa 1621 gaataacctt tcatctattc tgcctgattt tactgaatct gagaaaaagt tcatcaaagg 1681 aactgctgac ttttttgctc tttgctttgg acccaccttg agttttcaac ttttggaccc 1741 tcacatgaag ttccgccaat tggaatctcc caacctgagg caactgcttt cctggattga 1801 ccttgaattt aaccatcctc aaatatttat tgtggaaaat ggctggtttg tctcagggac 1861 caccaagaga gatgatgcca aatatatgta ttacctcaaa aagttcatca tggaaacctt 1921 aaaagccatc aagctggatg gggtggatgt catcgggtat accgcatggt ccctcatgga 1981 tggtttcgag tggcacagag gttacagcat caggcgtgga ctcttctatg ttgactttct 2041 aagccaggac aagatgttgt tgccaaagtc ttcagccttg ttctaccaaa agctgataga 2101 gaaaaatggc ttccctcctt tacctgaaaa tcagccccta gaagggacat ttccctgtga 2161 ctttgcttgg ggagttgttg acaactacat tcaagtagat accactctgt ctcagtttac 2221 cgacctgaat gtttacctgt gggatgtcca ccacagtaaa aggcttatta aagtggatgg 2281 ggttgtgacc aagaagagga aatcctactg tgttgacttt gctgccatcc agccccagat 2341 cgctttactc caggaaatgc acgttacaca ttttcgcttc tccctggact gggccctgat 2401 tctccctctg ggtaaccagt cccaggtgaa ccacaccatc ctgcagtact atcgctgcat 2461 ggccagcgag cttgtccgtg tcaacatcac cccagtggtg gccctgtggc agcctatggc 2521 cccgaaccaa ggactgccgc gcctcctggc caggcagggc gcctgggaga acccctacac 2581 tgccctggcc tttgcagagt atgcccgact gtgctttcaa gagctcggcc atcacgtcaa 2641 gctttggata acgatgaatg agccgtatac aaggaatatg acatacagtg ctggccacaa 2701 ccttctgaag gcccatgccc tggcttggca tgtgtacaat gaaaagttta ggcatgctca 2761 gaatgggaaa atatccatag ccttgcaggc tgattggata gaacctgcct gccctttctc 2821 ccaaaaggac aaagaggtgg ccgagagagt tttggaattt gacattggct ggctggctga 2881 gcccattttc ggctctggag attatccatg ggtgatgagg gactggctga accaaagaaa 2941 caattttctt cttccttatt tcactgaaga tgaaaaaaag ctaatccagg gtacctttga 3001 ctttttggct ttaagccatt ataccaccat ccttgtagac tcagaaaaag aagatccaat 3061 aaaatacaat gattacctag aagtgcaaga aatgaccgac atcacgtggc tcaactcccc 3121 cagtcaggtg gcggtagtgc cctgggggtt gcgcaaagtg ctgaactggc tgaagttcaa 3181 gtacggagac ctccccatgt acataatatc caacggaatc gatgacgggc tgcatgctga 3241 ggacgaccag ctgagggtgt attatatgca gaattacata aacgaagctc tcaaagccca 3301 catactggat ggtatcaatc tttgcggata ctttgcttat tcgtttaacg accgcacagc 3361 tccgaggttt ggcctctatc gttatgctgc agatcagttt gagcccaagg catccatgaa 3421 acattacagg aaaattattg acagcaatgg tttcccgggc ccagaaactc tggaaagatt 3481 ttgtccagaa gaattcaccg tgtgtactga gtgcagtttt tttcacaccc gaaagtcttt 3541 actggctttc atagcttttc tattttttgc ttctattatt tctctctccc ttatatttta 3601 ctactcgaag aaaggcagaa gaagttacaa aTAGGACCCA GCTTTCTTGT ACAAAGTTGG 3661 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 3721 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 3781 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 3841 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 3901 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 3961 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 4021 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 4081 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 4141 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 4201 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 4261 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 4321 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 4381 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 4441 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 4501 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 4561 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 4621 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 4681 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 4741 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 4801 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 4861 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 4921 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 4981 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 5041 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 5101 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 5161 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 5221 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 5281 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 5341 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 5401 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 5461 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //