LOCUS KLB-pGEn1-DEST 7300 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5928..6469 /note="WPRE" misc_recomb 5890..5912 /label=attB2 /note="attB2 site" misc_feature complement(6476..6690) /note="BGH pA" misc_feature complement(6778..6907) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5886 /dnas_title="KLB-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:132531 IMAGE:8143874" /tissue_type="Liver, PCR rescued clones" /clone_lib="NIH_MGC_291" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: GAAAGCCC" gene 3166..5886 /dnas_title="KLB" /gene="KLB" /db_xref="GeneID:152831" /db_xref="HGNC:15527" misc_feature 3166..5886 /dnas_title="KLB" /gene="KLB" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 3166..5886 /dnas_title="klotho beta" /gene="KLB" /codon_start=1 /product="klotho beta" /protein_id="AAI04872.1" /db_xref="GI:85396950" /db_xref="GeneID:152831" /db_xref="HGNC:15527" /translation="MKPGCAAGSPGNEWIFFSTDEITTRYRNTMSNGGLQRSVILSAL ILLRAVTGFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSWKK DGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQFSISWPRLFP DGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPLALQEKYGGWKNDTIIDIF NDYATYCFQMFGDRVKYWITIHNPYLVAWHGYGTGMHAPGEKGNLAAVYTVGHNLIKA HSKVWHNYNTHFRPHQKGWLSITLGSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPI HGDGDYPEGMRKKLFSVLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNV SLNLREALNWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLD EIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYKQIIRENGF SLKESTPDVQGQFPCDFSWGVTESVLKPESVASSPQFSDPHLYVWNATGNRLLHRVEG VRLKTRPAQCTDFVNIKKQLEMLARMKVTHYRFALDWASVLPTGNLSAVNRQALRYYR CVVSEGLKLGISAMVTLYYPTHAHLGLPEPLLHADGWLNPSTAEAFQAYAGLCFQELG DLVKLWITINEPNRLSDIYNRSGNDTYGAAHNLLVAHALAWRLYDRQFRPSQRGAVSL SLHADWAEPANPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGL SSSALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFLQDITRL SSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDDRLRKYYLGKYLQEV LKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFKAKSSIQFYNKVISSRGFPFENS SSRCSQTQENTECTVCLFLVQKKPLIFLGCCFFSTLVLLLSIAIFQRQKRRKFWKAKN LQHIPLKKGKRVVS" misc_feature 3166..5886 /note="KLB coding region" vector join(5898..7300,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5897 /source="KLB-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggctatga cactttccct 3181 aaaaactttt tctggggtat tgggactgga gcattgcaag tggaagggag ttggaagaag 3241 gatggaaaag gaccttctat atgggatcat ttcatccaca cacaccttaa aaatgtcagc 3301 agcacgaatg gttccagtga cagttatatt tttctggaaa aagacttatc agccctggat 3361 tttataggag tttcttttta tcaattttca atttcctggc caaggctttt ccccgatgga 3421 atagtaacag ttgccaacgc aaaaggtctg cagtactaca gtactcttct ggacgctcta 3481 gtgcttagaa acattgaacc tatagttact ttataccact gggatttgcc tttggcacta 3541 caagaaaaat atggggggtg gaaaaatgat accataatag atatcttcaa tgactatgcc 3601 acatactgtt tccagatgtt tggggaccgt gtcaaatatt ggattacaat tcacaaccca 3661 tatctagtgg cttggcatgg gtatgggaca ggtatgcatg cccctggaga gaagggaaat 3721 ttagcagctg tctacactgt gggacacaac ttgatcaagg ctcactcgaa agtttggcat 3781 aactacaaca cacatttccg cccacatcag aagggttggt tatcgatcac gttgggatct 3841 cattggatcg agccaaaccg gtcggaaaac acgatggata tattcaaatg tcaacaatcc 3901 atggtttctg tgcttggatg gtttgccaac cctatccatg gggatggcga ctatccagag 3961 gggatgagaa agaagttgtt ctccgttcta cccattttct ctgaagcaga gaagcatgag 4021 atgagaggca cagctgattt ctttgccttt tcttttggac ccaacaactt caagccccta 4081 aacaccatgg ctaaaatggg acaaaatgtt tcacttaatt taagagaagc gctgaactgg 4141 attaaactgg aatacaacaa ccctcgaatc ttgattgctg agaatggctg gttcacagac 4201 agtcgtgtga aaacagaaga caccacggcc atctacatga tgaagaattt cctcagccag 4261 gtgcttcaag caataaggtt agatgaaata cgagtgtttg gttatactgc ctggtctctc 4321 ctggatggct ttgaatggca ggatgcttac accatccgcc gaggattatt ttatgtggat 4381 tttaacagta aacagaaaga gcggaaacct aagtcttcag cacactacta caaacagatc 4441 atacgagaaa atggtttttc tttaaaagag tccacgccag atgtgcaggg ccagtttccc 4501 tgtgacttct cctggggtgt cactgaatct gttcttaagc ccgagtctgt ggcttcgtcc 4561 ccacagttca gcgatcctca tctgtacgtg tggaacgcca ctggcaacag actgttgcac 4621 cgagtggaag gggtgaggct gaaaacacga cccgctcaat gcacagattt tgtaaacatc 4681 aaaaaacaac ttgagatgtt ggcaagaatg aaagtcaccc actaccggtt tgctctggat 4741 tgggcctcgg tccttcccac tggcaacctg tccgcggtga accgacaggc cctgaggtac 4801 tacaggtgcg tggtcagtga ggggctgaag cttggcatct ccgcgatggt caccctgtat 4861 tatccgaccc acgcccacct aggcctcccc gagcctctgt tgcatgccga cgggtggctg 4921 aacccatcga cggccgaggc cttccaggcc tacgctgggc tgtgcttcca ggagctgggg 4981 gacctggtga agctctggat caccatcaac gagcctaacc ggctaagtga catctacaac 5041 cgctctggca acgacaccta cggggcggcg cacaacctgc tggtggccca cgccctggcc 5101 tggcgcctct acgaccggca gttcaggccc tcacagcgcg gggccgtgtc gctgtcgctg 5161 cacgcggact gggcggaacc cgccaacccc tatgctgact cgcactggag ggcggccgag 5221 cgcttcctgc agttcgagat cgcctggttc gccgagccgc tcttcaagac cggggactac 5281 cccgcggcca tgagggaata cattgcctcc aagcaccgac gggggctttc cagctcggcc 5341 ctgccgcgcc tcaccgaggc cgaaaggagg ctgctcaagg gcacggtcga cttctgcgcg 5401 ctcaaccact tcaccactag gttcgtgatg cacgagcagc tggccggcag ccgctacgac 5461 tcggacaggg acatccagtt tctgcaggac atcacccgcc tgagctcccc cacgcgcctg 5521 gctgtgattc cctggggggt gcgcaagctg ctgcggtggg tccggaggaa ctacggcgac 5581 atggacattt acatcaccgc cagtggcatc gacgaccagg ctctggagga tgaccggctc 5641 cggaagtact acctagggaa gtaccttcag gaggtgctga aagcatacct gattgataaa 5701 gtcagaatca aaggctatta tgcattcaaa ctggctgaag agaaatctaa acccagattt 5761 ggattcttca catctgattt taaagctaaa tcctcaatac aattttacaa caaagtgatc 5821 agcagcaggg gcttcccttt tgagaacagt agttctagat gcagtcagac ccaagaaaat 5881 acagagtgaC CAGCTTTCTT GTACAAAGTG GTTGATAGCT TGGATCCAAT CAACCTCTGG 5941 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 6001 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 6061 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 6121 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 6181 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 6241 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 6301 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 6361 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 6421 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC 6481 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 6541 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 6601 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 6661 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA 6721 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG 6781 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG 6841 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG 6901 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC 6961 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 7021 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 7081 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 7141 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT 7201 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 7261 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //