LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2240..2336) /label=attL2 /note="attL2 (c)" misc_signal complement(2351..2370) /note="T7 Promoter/priming site (c)" primer_bind 2378..2394 /note="M13 Reverse priming site" gene 2507..3316 /note="Kanamycin resistance gene" rep_origin 3437..4110 /note="pUC origin" vector join(2248..4113,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2239 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100015340" /clone_lib="NIH_MGC_425" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 without stop codon" source 692..2236 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..2236 /gene="LCTL" /gene_synonym=KLG /gene_synonym=KLPH /db_xref="GeneID:197021" /db_xref="HGNC:15583" CDS 692..2236 /dnas_title="lactase-like" /gene="LCTL" /gene_synonym=KLG /gene_synonym=KLPH /codon_start=1 /product="lactase-like" /protein_id="AAI48355.1" /db_xref="GI:151556442" /db_xref="GeneID:197021" /db_xref="HGNC:15583" /translation="MKPVWVATLLWMLLLVPRLGAARKGSPEEASFYYGTFPLGFSWG VGSSAYQTEGAWDQDGKGPSIWDVFTHSGKGKVLGNETADVACDGYYKVQEDIILLRE LHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLIDALLSSNITPIVTLHHWDLPQ LLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGYETGHHAPG LKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKDL EAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKG TSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFR RLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANIK GYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPRE VESWYLKALETCSINNQMLAAEPLLSHMQMVTEIVVPTVCSLCVLITAVLLMLLLRRQ S" misc_feature 692..2239 /note="LCTL coding region" misc_feature 2237..2239 /note="linker at the 3' end of the ORF" misc_difference 2238..2239 /note="29 bases at the 3' end do not align to the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cccagaagag gcctccttct actatggaac 721 cttccctctt ggcttctcct ggggcgtggg cagttctgcc taccagacgg agggcgcctg 781 ggaccaggac gggaaagggc ctagcatctg ggacgtcttc acacacagtg ggaaggggaa 841 agtgcttggg aatgagacgg cagatgtagc ctgtgacggc tactacaagg tccaggagga 901 catcattctg ctgagggaac tgcacgtcaa ccactaccga ttctccctgt cttggccccg 961 gctcctgccc acaggcatcc gagccgagca ggtgaacaag aagggaatcg aattctacag 1021 tgatcttatc gatgcccttc tgagcagcaa catcactccc atcgtgacct tgcaccactg 1081 ggatctgcca cagctgctcc aggtcaaata cggtgggtgg cagaatgtga gcatggccaa 1141 ctacttcaga gactacgcca acctgtgctt tgaggccttt ggggaccgtg tgaagcactg 1201 gatcacgttc agtgatcctc gggcaatggc agaaaaaggc tatgagacgg gccaccatgc 1261 gccgggcctg aagctccgcg gcaccggcct gtacaaggca gcacaccaca tcattaaggc 1321 ccacgccaaa gcctggcatt cttataacac cacgtggcgc agcaagcagc aaggtctggt 1381 gggaatttca ttgaactgtg actgggggga acctgtggac attagtaacc ccaaggacct 1441 agaggctgcc gagagatacc tacagttctg tctgggctgg tttgccaacc ccatttatgc 1501 cggtgactac ccccaagtca tgaaggacta cattggaaga aagagtgcag agcaaggcct 1561 ggagatgtcg aggttaccgg tgttctcact ccaggagaag agctacatta aaggcacatc 1621 cgatttcttg ggattaggtc attttactac tcggtacatc acggaaagga actacccctc 1681 ccgccagggg cccagctacc agaacgatcg tgacttgata gagctggttg acccaaactg 1741 gccagatctg gggtctaaat ggctatattc tgtgccatgg ggatttagga ggctccttaa 1801 ctttgctcag actcaatacg gtgatcctcc catatatgtg atggaaaatg gagcatctca 1861 aaaattccac tgtactcaat tatgtgatga gtggagaatt caatacctta aaggatacat 1921 aaatgaaatg ctaaaagcta taaaagatgg tgctaatata aaggggtata cttcctggtc 1981 tctgttggat aagtttgaat gggagaaagg atactcagat agatatggat tctactatgt 2041 tgaatttaac gacagaaata agcctcgcta tccaaaggct tcagttcaat attacaagaa 2101 gattatcatt gccaatgggt ttcccaatcc aagagaggtg gaaagttggt acctcaaagc 2161 tttggaaact tgctctatca acaatcagat gcttgctgca gagcccttgc taagtcacat 2221 gcaaatggtt acggagtgaC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2281 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2341 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2401 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2461 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2521 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 2581 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 2641 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 2701 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 2761 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 2821 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 2881 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 2941 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 3001 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 3061 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 3121 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 3181 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3241 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3301 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3361 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3421 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3481 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3541 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3601 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3661 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3721 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 3781 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 3841 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 3901 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 3961 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4021 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4081 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //