LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2801..2897) /label=attL2 /note="attL2 (c)" misc_signal complement(2912..2931) /note="T7 Promoter/priming site (c)" primer_bind 2939..2955 /note="M13 Reverse priming site" gene 3068..3877 /note="Kanamycin resistance gene" rep_origin 3998..4671 /note="pUC origin" vector join(2809..4674,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2800 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:20071 IMAGE:4574938" /tissue_type="Primary B-Cells from Tonsils" /clone_lib="NIH_MGC_48" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2800 /gene="GBE1" /gene_synonym=GBE /db_xref="GeneID:2632" /db_xref="HGNC:4180" /db_xref="MIM:607839" CDS 692..2800 /dnas_title="glucan (1,4-alpha-), branching enzyme 1" /gene="GBE1" /gene_synonym=GBE /codon_start=1 /product="glucan (1,4-alpha-), branching enzyme 1" /protein_id="AAH12098.1" /db_xref="GI:15082371" /db_xref="GeneID:2632" /db_xref="HGNC:4180" /db_xref="MIM:607839" /translation="MAAPMTPAARPEDYEAALNAALADVPELARLLEIDPYLKPYAVD FQRRYKQFSQILKNIGENEGGIDKFSRGYESFGVHRCADGGLYCKEWAPGAEGVFLTG DFNGWNPFSYPYKKLDYGKWELYIPPKQNKSVLVPHGSKLKVVITSKSGEILYRISPW AKYVVREGDNVNYDWIHWDPEHSYEFKHSRPKKPRSLRIYESHVGISSHEGKVASYKH FTCNVLPRIKGLGYNCIQLMAIMEHAYYASFGYQITSFFAASSRYGSPEELQELVDTA HSMGIIVLLDVVHSHASKNSADGLNMFDGTDSCYFHSGPRGTHDLWDSRLFAYSSWEV LRFLLSNIRWWLEEYRFDGFRFDGVTSMLYHHHGVGQGFSGDYSEYFGLQVDEDALTY LMLANHLVHTLCPDSITIAEDVSGMPALCSPISQGGGGFDYRLAMAIPDKWIQLLKEF KDEDWNMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSLAFWLMDAEMYTNMSVLTP FTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWLDFPRKGNNESYHYARRQ FHLTDDDLLRYKFLNNFDRDMNRLEERYGWLAAPQAYVSEKHEGNKIIAFERAGLLFI FNFHPSKSYTDYRVGTALPGKFKIVLDSDAAEYGGHQRLDHSTDFFSEAFEHNGRPYS LLVYIPSRVALILQNVDLPN" misc_feature 692..2800 /note="GBE1 coding region" misc_difference 1484..1484 /gene="GBE1" /gene_synonym=GBE /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'S' in cDNA, 'T' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1691..1691 /gene="GBE1" /gene_synonym=GBE /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catggcggct ccgatgactc ccgcggctcg 721 gcccgaggac tacgaggcgg cgctcaatgc cgccctggct gacgtgcccg aactggccag 781 actcctggag atcgacccgt acttgaagcc ctacgccgtg gacttccagc gcaggtataa 841 gcagtttagc caaattttga agaacattgg agaaaatgaa ggtggtattg ataagttttc 901 cagaggctat gaatcatttg gcgtccacag atgtgctgat ggtggtttat actgcaaaga 961 atgggccccg ggagcagaag gagtttttct tactggagat tttaatggtt ggaatccatt 1021 ttcgtaccca tacaaaaaac tggattatgg aaaatgggag ctgtatatcc caccaaagca 1081 gaataaatct gtactcgtgc ctcatggatc caaattaaag gtagttatta ctagtaaaag 1141 cggagagatc ttgtatcgta tttcaccgtg ggcaaagtat gtggttcgtg aaggtgataa 1201 tgtgaattat gattggatac actgggatcc agaacactca tatgagttta agcattccag 1261 accaaagaag ccacggagtc taagaattta tgaatctcat gtgggaattt cttcccatga 1321 aggaaaagta gcttcttata aacattttac atgcaatgta ctaccaagaa tcaaaggcct 1381 tggatacaac tgcattcagt tgatggcaat catggagcat gcttactatg ccagctttgg 1441 ttaccaaatc acaagcttct ttgcagcttc cagccgttat ggatcacctg aagagctaca 1501 agaactggta gacacagctc attccatggg tatcatagtc ctcttagatg tggtacacag 1561 ccatgcttca aaaaattcag cagatggatt gaatatgttt gatgggacag attcctgtta 1621 ttttcattct ggacctagag ggactcatga tctttgggat agcagattgt ttgcctactc 1681 cagctgggaa gttttaagat tccttctgtc aaacataaga tggtggttgg aagaatatcg 1741 ctttgatgga tttcgttttg atggtgttac gtccatgctt tatcatcacc atggagtggg 1801 tcaaggtttc tcaggtgatt acagtgaata tttcggacta caagtagatg aagatgcctt 1861 gacttacctc atgttggcaa atcatttggt tcacacgctg tgtcccgatt ctataacaat 1921 agctgaggat gtatcaggaa tgccagctct gtgctctcca atttcccagg gagggggtgg 1981 ttttgactat cgactagcca tggcaattcc agataagtgg attcagctac ttaaagagtt 2041 taaagatgaa gactggaaca tgggcgatat agtatacacg ctcacaaaca ggcgctacct 2101 tgaaaagtgc attgcttatg cagagagcca tgatcaggca ttggttgggg ataagtcgct 2161 ggcattttgg ttgatggatg ccgaaatgta tacaaacatg agtgtcctga ctccttttac 2221 tccagttatt gatcgtggaa tacagcttca taaaatgatt cgactcatta cgcatgggct 2281 tggtggagaa ggctatctca atttcatggg taatgaattt gggcatcctg aatggttaga 2341 cttcccaaga aaaggaaata atgagagtta ccattatgcc aggcggcagt ttcatttaac 2401 tgacgacgac cttcttcgct acaagttcct aaataatttt gacagggata tgaatagatt 2461 ggaagaaaga tatggttggc ttgcagctcc acaggcctac gtgagtgaaa aacatgaagg 2521 caataagatc attgcttttg aaagagcagg tcttcttttc attttcaact tccatccaag 2581 caagagctac actgactacc gagttggaac agcattgcca gggaaattca aaattgtgct 2641 agattcagat gcagcggaat atggagggca tcagagactg gaccacagca ctgacttttt 2701 ttctgaggct tttgaacata atgggcgtcc ctattctctt ttggtgtaca ttccaagcag 2761 agtggccctc atccttcaga atgtggatct gccgaattga CCAGCTTTCT TGTACAAAGT 2821 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 2881 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 2941 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 3001 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 3061 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 3121 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 3181 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 3241 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 3301 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 3361 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 3421 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 3481 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 3541 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 3601 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 3661 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 3721 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 3781 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 3841 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 3901 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 3961 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 4021 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 4081 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 4141 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 4201 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 4261 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 4321 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 4381 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 4441 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 4501 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 4561 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 4621 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //