LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2420..2516) /label=attL2 /note="attL2 (c)" misc_signal complement(2531..2550) /note="T7 Promoter/priming site (c)" primer_bind 2558..2574 /note="M13 Reverse priming site" gene 2687..3496 /note="Kanamycin resistance gene" rep_origin 3617..4290 /note="pUC origin" vector join(2428..4293,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2419 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:24059 IMAGE:4556946" /tissue_type="Kidney" /clone_lib="NIH_MGC_75" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 692..2419 /gene="SLC3A1" /gene_synonym=ATR1 /gene_synonym=CSNU1 /gene_synonym=D2H /gene_synonym=NBAT /gene_synonym=RBAT /db_xref="GeneID:6519" /db_xref="HGNC:11025" /db_xref="MIM:104614" CDS 692..2419 /dnas_title="solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1" /gene="SLC3A1" /gene_synonym=ATR1 /gene_synonym=CSNU1 /gene_synonym=D2H /gene_synonym=NBAT /gene_synonym=RBAT /codon_start=1 /product="solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1" /protein_id="AAH22386.1" /db_xref="GI:18490867" /db_xref="GeneID:6519" /db_xref="HGNC:11025" /db_xref="MIM:104614" /translation="MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNL KHSTRGILGSQEPDFKGVQPYAGMPKEVLFQFSGQARYRIPREILFWLTVASVLVLIA ATIAIIALSPKCLDWWQEGPMYQIYPRSFKDSNKDGNGDLKGIQDKLDYITALNIKTV WITSFYKSSLKDFRYGVEDFREVDPIFGTMEDFENLVAAIHDKGLKLIIDFIPNHTSD KHIWFQLSRTRTGKYTDYYIWHDCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFH QFMKEQPDLNFRNPDVQEEIKEILRFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKT QIPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDR TVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDS SRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSP MQWDNSSNAGFSEASNTWLPTNSDYHTVNVDVQKTQPRSALKLYQDLSLLHANELLLN RGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFGESTLLNLHNMISGLPAKIRIRLST NSADKGSKVDTSGIFLDKGEGLIFEHNTKNLLHRQTAFRDRCFVSNRACYSSVLNILY TSC" misc_feature 692..2419 /note="SLC3A1 coding region" misc_difference 2215..2215 /gene="SLC3A1" /gene_synonym=ATR1 /gene_synonym=CSNU1 /gene_synonym=D2H /gene_synonym=NBAT /gene_synonym=RBAT /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'I' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctctccaaag tgcctagact ggtggcagga 721 ggggcccatg taccagatct acccaaggtc tttcaaggac agtaacaagg atgggaacgg 781 agatctgaaa ggtattcaag ataaactgga ctacatcaca gctttaaata taaaaactgt 841 ttggattact tcattttata aatcgtccct taaagatttc agatatggtg ttgaagattt 901 ccgggaagtt gatcccattt ttggaacgat ggaagatttt gagaatctgg ttgcagccat 961 acatgataaa ggtttaaaat taatcatcga tttcatacca aaccacacga gtgataaaca 1021 tatttggttt caattgagtc ggacacggac aggaaaatat actgattatt atatctggca 1081 tgactgtacc catgaaaatg gcaaaaccat tccacccaac aactggttaa gtgtgtatgg 1141 aaactccagt tggcactttg acgaagtgcg aaaccaatgt tattttcatc agtttatgaa 1201 agagcaacct gatttaaatt tccgcaatcc tgatgttcaa gaagaaataa aagaaatttt 1261 acggttctgg ctcacaaagg gtgttgatgg ttttagtttg gatgctgtta aattcctcct 1321 agaagcaaag cacctgagag atgagatcca agtaaataag acccaaatcc cggacacggt 1381 cacacaatac tcggagctgt accatgactt caccaccacg caggtgggaa tgcacgacat 1441 tgtccgcagc ttccggcaga ccatggacca atacagcacg gagcccggca gatacaggtt 1501 catggggact gaagcctatg cagagagtat tgacaggacc gtgatgtact atggattgcc 1561 atttatccaa gaagctgatt ttcccttcaa caattacctc agcatgctag acactgtttc 1621 tgggaacagc gtgtatgagg ttatcacatc ctggatggaa aacatgccag aaggaaaatg 1681 gcctaactgg atgattggtg gaccagacag ttcacggctg acttcgcgtt tggggaatca 1741 gtatgtcaac gtgatgaaca tgcttctttt cacactccct ggaactccta taacttacta 1801 tggagaagaa attggaatgg gaaatattgt agccgcaaat ctcaatgaaa gctatgatat 1861 taataccctt cgctcaaagt caccaatgca gtgggacaat agttcaaatg ctggtttttc 1921 tgaagctagt aacacctggt tacctaccaa ttcagattac cacactgtga atgttgatgt 1981 ccaaaagact cagcccagat cggctttgaa gttatatcaa gatttaagtc tacttcatgc 2041 caatgagcta ctcctcaaca ggggctggtt ttgccatttg aggaatgaca gccactatgt 2101 tgtgtacaca agagagctgg atggcatcga cagaatcttt atcgtggttc tgaattttgg 2161 agaatcaaca ctgttaaatc tacataatat gatttcgggc cttcccgcta aaataagaat 2221 aaggttaagt accaattctg ccgacaaagg cagtaaagtt gatacaagtg gcatttttct 2281 ggacaaggga gagggactca tctttgaaca caacacgaag aatctccttc atcgccaaac 2341 agctttcaga gatagatgct ttgtttccaa tcgagcatgc tattccagtg tactgaacat 2401 actgtatacc tcgtgttagC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2461 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2521 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2581 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2641 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2701 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 2761 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 2821 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 2881 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 2941 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 3001 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 3061 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 3121 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 3181 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 3241 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 3301 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 3361 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3421 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3481 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3541 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3601 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3661 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3721 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3781 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3841 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3901 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 3961 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4021 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4081 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4141 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4201 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4261 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //