LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1844..1940) /label=attL2 /note="attL2 (c)" misc_signal complement(1955..1974) /note="T7 Promoter/priming site (c)" primer_bind 1982..1998 /note="M13 Reverse priming site" gene 2111..2920 /note="Kanamycin resistance gene" rep_origin 3041..3714 /note="pUC origin" PCR_frame 669..1844 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1864) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1861) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1852..3717,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1822..1842 /note="TEV site" source 673..1821 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17093 IMAGE:4212748" /tissue_type="Brain, glioblastoma with EGFR amplification" /clone_lib="NCI_CGAP_Brn64" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1821 /gene="CHI3L1" /gene_synonym=GP39 /gene_synonym=HC-gp39 /gene_synonym=HCGP-3P /gene_synonym=YKL40 /db_xref="GeneID:1116" /db_xref="HGNC:1932" /db_xref="MIM:601525" CDS 673..1821 /dnas_title="chitinase 3-like 1 (cartilage glycoprotein-39)" /gene="CHI3L1" /gene_synonym=GP39 /gene_synonym=HC-gp39 /gene_synonym=HCGP-3P /gene_synonym=YKL40 /codon_start=1 /product="chitinase 3-like 1 (cartilage glycoprotein-39)" /protein_id="AAH08568.1" /db_xref="GI:14919433" /db_xref="GeneID:1116" /db_xref="HGNC:1932" /db_xref="MIM:601525" /translation="MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPD ALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFG SQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAE FIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGH HSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGA PISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESV KSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT" misc_feature 673..1821 /note="CHI3L1 coding region" misc_difference 1764..1764 /gene="CHI3L1" /gene_synonym=GP39 /gene_synonym=HC-gp39 /gene_synonym=HCGP-3P /gene_synonym=YKL40 /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgggtgt gaaggcgtct caaacaggct ttgtggtcct ggtgctgctc 721 cagtgctgct ctgcatacaa actggtctgc tactacacca gctggtccca gtaccgggaa 781 ggcgatggga gctgcttccc agatgccctt gaccgcttcc tctgtaccca catcatctac 841 agctttgcca atataagcaa cgatcacatc gacacctggg agtggaatga tgtgacgctc 901 tacggcatgc tcaacacact caagaacagg aaccccaacc tgaagactct cttgtctgtc 961 ggaggatgga actttgggtc tcaaagattt tccaagatag cctccaacac ccagagtcgc 1021 cggactttca tcaagtcagt accgccattt ctgcgcaccc atggctttga tgggctggac 1081 cttgcctggc tctaccctgg acggagagac aaacagcatt ttaccaccct aatcaaggaa 1141 atgaaggccg aatttataaa ggaagcccag ccagggaaaa agcagctcct gctcagcgca 1201 gcactgtctg cggggaaggt caccattgac agcagctatg acattgccaa gatatcccaa 1261 cacctggatt tcattagcat catgacctac gattttcatg gagcctggcg tgggaccaca 1321 ggccatcaca gtcccctgtt ccgaggtcag gaggatgcaa gtcctgacag attcagcaac 1381 actgactatg ctgtggggta catgttgagg ctgggggctc ctgccagtaa gctggtgatg 1441 ggcatcccca ccttcgggag gagcttcact ctggcttctt ctgagactgg tgttggagcc 1501 ccaatctcag gaccgggaat tccaggccgg ttcaccaagg aggcagggac ccttgcctac 1561 tatgagatct gtgacttcct ccgcggagcc acagtccata gaatcctcgg ccagcaggtc 1621 ccctatgcca ccaagggcaa ccagtgggta ggatacgacg accaggaaag cgtcaaaagc 1681 aaggtgcagt acctgaagga caggcagctg gcgggcgcca tggtatgggc cctggacctg 1741 gatgacttcc agggctcctt ctgcggccag gatctgcgct tccctctcac caatgccatc 1801 aaggatgcac tcgctgcaac ggaaaacttg tactttcaag gctCCAGCTT TCTTGTACAA 1861 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 1921 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT 1981 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC 2041 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 2101 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT 2161 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC 2221 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG 2281 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT 2341 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC 2401 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA 2461 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG 2521 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG 2581 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG 2641 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT 2701 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG 2761 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT 2821 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA 2881 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT 2941 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC 3001 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3061 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3121 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3181 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 3241 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3301 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3361 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3421 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 3481 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 3541 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 3601 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 3661 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT //