LOCUS CHI3L1-pGEc2-DES 6370 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5848..5977) /note="3'-ITR" misc_feature complement(5546..5760) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4998..5539 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4177..4199 /note="attB2" misc_feature 4977..4982 /note="BstB1 site" misc_feature 4200..4895 /note="Super GFP" misc_feature 4896..4946 /note="AviTag" misc_feature 4947..4970 /note="His Tag" PCR_frame 3002..4177 /note="Reading Frame" /set="Set 1" source 3006..4154 /dnas_title="CHI3L1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17093 IMAGE:4212748" /tissue_type="Brain, glioblastoma with EGFR amplification" /clone_lib="NCI_CGAP_Brn64" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4154 /dnas_title="CHI3L1" /gene="CHI3L1" /gene_synonym="GP39" /gene_synonym="HC-gp39" /gene_synonym="HCGP-3P" /gene_synonym="YKL40" /db_xref="GeneID:1116" /db_xref="HGNC:1932" /db_xref="MIM:601525" CDS 3006..4154 /dnas_title="chitinase 3-like 1 (cartilage glycoprotein-39)" /gene="CHI3L1" /gene_synonym="GP39" /gene_synonym="HC-gp39" /gene_synonym="HCGP-3P" /gene_synonym="YKL40" /codon_start=1 /product="chitinase 3-like 1 (cartilage glycoprotein-39)" /protein_id="AAH08568.1" /db_xref="GI:14919433" /db_xref="GeneID:1116" /db_xref="HGNC:1932" /db_xref="MIM:601525" /translation="MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPD ALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFG SQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAE FIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGH HSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGA PISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESV KSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT" misc_feature 3006..4154 /note="CHI3L1 coding region" misc_feature 4155..4175 /note="TEV site" misc_difference 4097..4097 /dnas_title="CHI3L1" /gene="CHI3L1" /gene_synonym="GP39" /gene_synonym="HC-gp39" /gene_synonym="HCGP-3P" /gene_synonym="YKL40" /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4184) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4184) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4185..6370,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4184 /source="CHI3L1-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggg tgtgaaggcg tctcaaacag gctttgtggt cctggtgctg ctccagtgct 3061 gctctgcata caaactggtc tgctactaca ccagctggtc ccagtaccgg gaaggcgatg 3121 ggagctgctt cccagatgcc cttgaccgct tcctctgtac ccacatcatc tacagctttg 3181 ccaatataag caacgatcac atcgacacct gggagtggaa tgatgtgacg ctctacggca 3241 tgctcaacac actcaagaac aggaacccca acctgaagac tctcttgtct gtcggaggat 3301 ggaactttgg gtctcaaaga ttttccaaga tagcctccaa cacccagagt cgccggactt 3361 tcatcaagtc agtaccgcca tttctgcgca cccatggctt tgatgggctg gaccttgcct 3421 ggctctaccc tggacggaga gacaaacagc attttaccac cctaatcaag gaaatgaagg 3481 ccgaatttat aaaggaagcc cagccaggga aaaagcagct cctgctcagc gcagcactgt 3541 ctgcggggaa ggtcaccatt gacagcagct atgacattgc caagatatcc caacacctgg 3601 atttcattag catcatgacc tacgattttc atggagcctg gcgtgggacc acaggccatc 3661 acagtcccct gttccgaggt caggaggatg caagtcctga cagattcagc aacactgact 3721 atgctgtggg gtacatgttg aggctggggg ctcctgccag taagctggtg atgggcatcc 3781 ccaccttcgg gaggagcttc actctggctt cttctgagac tggtgttgga gccccaatct 3841 caggaccggg aattccaggc cggttcacca aggaggcagg gacccttgcc tactatgaga 3901 tctgtgactt cctccgcgga gccacagtcc atagaatcct cggccagcag gtcccctatg 3961 ccaccaaggg caaccagtgg gtaggatacg acgaccagga aagcgtcaaa agcaaggtgc 4021 agtacctgaa ggacaggcag ctggcgggcg ccatggtatg ggccctggac ctggatgact 4081 tccagggctc cttctgcggc caggatctgc gcttccctct caccaatgcc atcaaggatg 4141 cactcgctgc aacggaaaac ttgtactttc aaggctCCAG CTTTCTTGTA CAAAGTGGTA 4201 TGAGCAAGGG CGAGGAACTG TTCACCGGCG TGGTGCCCAT CCTGGTGGAA CTGGATGGCG 4261 ACGTGAACGG CCACAAGTTC TCTGTGCGGG GAGAGGGCGA AGGCGACGCC ACAAATGGCA 4321 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCTTGG CCTACCCTCG 4381 TGACCACACT GACCTACGGC GTGCAGTGCT TCAGCAGATA CCCCGACCAT ATGAAGCGGC 4441 ACGACTTCTT CAAGAGCGCC ATGCCCGAGG GCTACGTGCA GGAACGGACC ATCAGCTTCA 4501 AGGACGACGG CACCTACAAG ACCAGAGCCG AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA 4561 ACCGGATCGA GCTGAAGGGC ATCGATTTCA AAGAGGACGG CAACATCCTG GGCCACAAGC 4621 TGGAGTACAA CTTCAACAGC CACAACGTGT ACATCACCGC CGACAAGCAG AAGAACGGCA 4681 TCAAGGCCAA CTTCAAGATC CGGCACAACG TGGAAGATGG CAGCGTGCAG CTGGCCGACC 4741 ACTACCAGCA GAACACCCCC ATCGGAGATG GCCCCGTGCT GCTGCCCGAC AACCACTACC 4801 TGAGCACCCA GAGCGTGCTG AGCAAGGACC CCAACGAGAA GCGGGACCAC ATGGTGCTGC 4861 TGGAATTCGT GACCGCCGCT GGCATCACCC ACGGCATGAG CGGCCTGAAC GACATCTTCG 4921 AGGCCCAGAA AATCGAGTGG CACGAGCACC ACCATCATCA CCATCACCAC TGATGATTCG 4981 AAAAATAATA AGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 5041 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 5101 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 5161 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 5221 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 5281 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 5341 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT 5401 CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 5461 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 5521 GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 5581 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 5641 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 5701 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 5761 CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT 5821 AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT 5881 CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT 5941 TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC 6001 GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT 6061 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC 6121 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA 6181 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT 6241 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 6301 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 6361 TACAATTTAG //