LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1781..1877) /label=attL2 /note="attL2 (c)" misc_signal complement(1892..1911) /note="T7 Promoter/priming site (c)" primer_bind 1919..1935 /note="M13 Reverse priming site" gene 2048..2857 /note="Kanamycin resistance gene" rep_origin 2978..3651 /note="pUC origin" vector join(1789..3654,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1780 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17093 IMAGE:4212748" /tissue_type="Brain, glioblastoma with EGFR amplification" /clone_lib="NCI_CGAP_Brn64" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1780 /gene="CHI3L1" /gene_synonym=GP39 /gene_synonym=HC-gp39 /gene_synonym=HCGP-3P /gene_synonym=YKL40 /db_xref="GeneID:1116" /db_xref="HGNC:1932" /db_xref="MIM:601525" CDS 692..1780 /dnas_title="chitinase 3-like 1 (cartilage glycoprotein-39)" /gene="CHI3L1" /gene_synonym=GP39 /gene_synonym=HC-gp39 /gene_synonym=HCGP-3P /gene_synonym=YKL40 /codon_start=1 /product="chitinase 3-like 1 (cartilage glycoprotein-39)" /protein_id="AAH08568.1" /db_xref="GI:14919433" /db_xref="GeneID:1116" /db_xref="HGNC:1932" /db_xref="MIM:601525" /translation="MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPD ALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFG SQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAE FIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGH HSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGA PISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESV KSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT" misc_feature 692..1780 /note="CHI3L1 coding region" misc_difference 1720..1720 /gene="CHI3L1" /gene_synonym=GP39 /gene_synonym=HC-gp39 /gene_synonym=HCGP-3P /gene_synonym=YKL40 /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctacaaactg gtctgctact acaccagctg 721 gtcccagtac cgggaaggcg atgggagctg cttcccagat gcccttgacc gcttcctctg 781 tacccacatc atctacagct ttgccaatat aagcaacgat cacatcgaca cctgggagtg 841 gaatgatgtg acgctctacg gcatgctcaa cacactcaag aacaggaacc ccaacctgaa 901 gactctcttg tctgtcggag gatggaactt tgggtctcaa agattttcca agatagcctc 961 caacacccag agtcgccgga ctttcatcaa gtcagtaccg ccatttctgc gcacccatgg 1021 ctttgatggg ctggaccttg cctggctcta ccctggacgg agagacaaac agcattttac 1081 caccctaatc aaggaaatga aggccgaatt tataaaggaa gcccagccag ggaaaaagca 1141 gctcctgctc agcgcagcac tgtctgcggg gaaggtcacc attgacagca gctatgacat 1201 tgccaagata tcccaacacc tggatttcat tagcatcatg acctacgatt ttcatggagc 1261 ctggcgtggg accacaggcc atcacagtcc cctgttccga ggtcaggagg atgcaagtcc 1321 tgacagattc agcaacactg actatgctgt ggggtacatg ttgaggctgg gggctcctgc 1381 cagtaagctg gtgatgggca tccccacctt cgggaggagc ttcactctgg cttcttctga 1441 gactggtgtt ggagccccaa tctcaggacc gggaattcca ggccggttca ccaaggaggc 1501 agggaccctt gcctactatg agatctgtga cttcctccgc ggagccacag tccatagaat 1561 cctcggccag caggtcccct atgccaccaa gggcaaccag tgggtaggat acgacgacca 1621 ggaaagcgtc aaaagcaagg tgcagtacct gaaggacagg cagctggcgg gcgccatggt 1681 atgggccctg gacctggatg acttccaggg ctccttctgc ggccaggatc tgcgcttccc 1741 tctcaccaat gccatcaagg atgcactcgc tgcaacgtag CCAGCTTTCT TGTACAAAGT 1801 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 1861 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 1921 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 1981 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 2041 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 2101 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2161 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2221 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 2281 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 2341 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 2401 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 2461 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 2521 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 2581 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 2641 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 2701 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 2761 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 2821 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 2881 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 2941 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3001 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3061 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3121 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3181 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3241 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3301 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3361 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 3421 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3481 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3541 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3601 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //