LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1865..1961) /label=attL2 /note="attL2 (c)" misc_signal complement(1976..1995) /note="T7 Promoter/priming site (c)" primer_bind 2003..2019 /note="M13 Reverse priming site" gene 2132..2941 /note="Kanamycin resistance gene" rep_origin 3062..3735 /note="pUC origin" PCR_frame 669..1865 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1885) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1882) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1873..3738,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1843..1863 /note="TEV site" source 673..1842 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17183 IMAGE:4214121" /tissue_type="Brain, glioblastoma with EGFR amplification" /clone_lib="NCI_CGAP_Brn64" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1842 /gene="CHI3L2" /gene_synonym=YKL-39 /gene_synonym=YKL39 /db_xref="GeneID:1117" /db_xref="HGNC:1933" /db_xref="MIM:601526" CDS 673..1842 /dnas_title="chitinase 3-like 2" /gene="CHI3L2" /gene_synonym=YKL-39 /gene_synonym=YKL39 /codon_start=1 /product="chitinase 3-like 2" /protein_id="AAH11460.1" /db_xref="GI:15079252" /db_xref="GeneID:1117" /db_xref="HGNC:1933" /db_xref="MIM:601526" /translation="MGATTMDQKSLWAGVVVLLLLQGGSAYKLVCYFTNWSQDRQEPG KFTPENIDPFLCSHLIYSFASIENNKVIIKDKSEVMLYQTINSLKTKNPKLKILLSIG GYLFGSKGFHPMVDSSTSRLEFINSIILFLRNHNFDGLDVSWIYPDQKENTHFTVLIH ELAEAFQKDFTKSTKERLLLTVGVSAGRQMIDNSYQVEKLAKDLDFINLLSFDFHGSW EKPLITGHNSPLSKGWQDRGPSSYYNVEYAVGYWIHKGMPSEKVVMGIPTYGHSFTLA SAETTVGAPASGPGAAGPITESSGFLAYYEICQFLKGAKITRLQDQQVPYAVKGNQWV GYDDVKSMETKVQFLKNLNLGGAMIWSIDMDDFTGKSCNQGPYPLVQAVKRSLGSL" misc_feature 673..1842 /note="CHI3L2 coding region" misc_difference 1217..1217 /gene="CHI3L2" /gene_synonym=YKL-39 /gene_synonym=YKL39 /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'A' in the human genome." misc_difference 1638..1638 /gene="CHI3L2" /gene_synonym=YKL-39 /gene_synonym=YKL39 /note="'G' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgggagc aaccaccatg gaccagaagt ctctctgggc aggtgtagtg 721 gtcttgctgc ttctccaggg aggatctgcc tacaaactgg tttgctactt taccaactgg 781 tcccaggacc ggcaggaacc aggaaaattc acccctgaga atattgaccc cttcctatgc 841 tctcatctca tctattcatt cgccagcatc gaaaacaaca aggttatcat caaggacaag 901 agtgaagtga tgctctacca gaccatcaac agtctcaaaa ccaagaatcc caaactgaaa 961 attctcttgt ccattggagg gtacctgttt ggttccaaag ggttccaccc tatggtggat 1021 tcttctacat cacgcttgga attcattaac tccataatcc tgtttctgag gaaccataac 1081 tttgatggac tggatgtaag ctggatctac ccagatcaga aagaaaacac tcatttcact 1141 gtgctgattc atgagttagc agaagccttt cagaaggact tcacaaaatc caccaaggaa 1201 aggcttctct tgactgtggg cgtatctgca gggaggcaaa tgattgataa cagctatcaa 1261 gttgagaaac tggcaaaaga tctggatttc atcaacctcc tgtcctttga cttccatggg 1321 tcttgggaaa agccccttat cactggccac aacagccctc tgagcaaggg gtggcaggac 1381 agagggccaa gctcctacta caatgtggaa tatgctgtgg ggtactggat acataaggga 1441 atgccatcag agaaggtggt catgggcatc cccacatatg ggcactcctt cacactggcc 1501 tctgcagaaa ccaccgtggg ggcccctgcc tctggccctg gagctgctgg acccatcaca 1561 gagtcttcag gcttcctggc ctattatgag atctgccagt tcctgaaagg agccaagatc 1621 acgcggctcc aggatcagca ggttccctac gcagtcaagg ggaaccagtg ggtgggctat 1681 gatgatgtga agagtatgga gaccaaggtt cagttcttaa agaatttaaa cctgggagga 1741 gccatgatct ggtctattga catggatgac ttcactggca aatcctgcaa ccagggccct 1801 taccctcttg tccaagcagt caagagaagc cttggctccc tggaaaactt gtactttcaa 1861 ggctCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 1921 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 1981 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 2041 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 2101 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2161 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2221 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2281 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2341 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2401 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 2461 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 2521 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 2581 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 2641 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 2701 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 2761 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 2821 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 2881 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 2941 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 3001 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 3061 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3121 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3181 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3241 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3301 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3361 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3421 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 3481 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 3541 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 3601 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 3661 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 3721 GCCTTTTGCT CACATGTT //