LOCUS CHI3L2-pGEc2-DES 6391 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5869..5998) /note="3'-ITR" misc_feature complement(5567..5781) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5019..5560 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4198..4220 /note="attB2" misc_feature 4998..5003 /note="BstB1 site" misc_feature 4221..4916 /note="Super GFP" misc_feature 4917..4967 /note="AviTag" misc_feature 4968..4991 /note="His Tag" PCR_frame 3002..4198 /note="Reading Frame" /set="Set 1" source 3006..4175 /dnas_title="CHI3L2-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17183 IMAGE:4214121" /tissue_type="Brain, glioblastoma with EGFR amplification" /clone_lib="NCI_CGAP_Brn64" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4175 /dnas_title="CHI3L2" /gene="CHI3L2" /gene_synonym="YKL-39" /gene_synonym="YKL39" /db_xref="GeneID:1117" /db_xref="HGNC:1933" /db_xref="MIM:601526" CDS 3006..4175 /dnas_title="chitinase 3-like 2" /gene="CHI3L2" /gene_synonym="YKL-39" /gene_synonym="YKL39" /codon_start=1 /product="chitinase 3-like 2" /protein_id="AAH11460.1" /db_xref="GI:15079252" /db_xref="GeneID:1117" /db_xref="HGNC:1933" /db_xref="MIM:601526" /translation="MGATTMDQKSLWAGVVVLLLLQGGSAYKLVCYFTNWSQDRQEPG KFTPENIDPFLCSHLIYSFASIENNKVIIKDKSEVMLYQTINSLKTKNPKLKILLSIG GYLFGSKGFHPMVDSSTSRLEFINSIILFLRNHNFDGLDVSWIYPDQKENTHFTVLIH ELAEAFQKDFTKSTKERLLLTVGVSAGRQMIDNSYQVEKLAKDLDFINLLSFDFHGSW EKPLITGHNSPLSKGWQDRGPSSYYNVEYAVGYWIHKGMPSEKVVMGIPTYGHSFTLA SAETTVGAPASGPGAAGPITESSGFLAYYEICQFLKGAKITRLQDQQVPYAVKGNQWV GYDDVKSMETKVQFLKNLNLGGAMIWSIDMDDFTGKSCNQGPYPLVQAVKRSLGSL" misc_feature 3006..4175 /note="CHI3L2 coding region" misc_difference 3550..3550 /dnas_title="CHI3L2" /gene="CHI3L2" /gene_synonym="YKL-39" /gene_synonym="YKL39" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'A' in the human genome." misc_feature 4176..4196 /note="TEV site" misc_difference 3971..3971 /dnas_title="CHI3L2" /gene="CHI3L2" /gene_synonym="YKL-39" /gene_synonym="YKL39" /note="'G' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4205) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4205) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4206..6391,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4205 /source="CHI3L2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggg agcaaccacc atggaccaga agtctctctg ggcaggtgta gtggtcttgc 3061 tgcttctcca gggaggatct gcctacaaac tggtttgcta ctttaccaac tggtcccagg 3121 accggcagga accaggaaaa ttcacccctg agaatattga ccccttccta tgctctcatc 3181 tcatctattc attcgccagc atcgaaaaca acaaggttat catcaaggac aagagtgaag 3241 tgatgctcta ccagaccatc aacagtctca aaaccaagaa tcccaaactg aaaattctct 3301 tgtccattgg agggtacctg tttggttcca aagggttcca ccctatggtg gattcttcta 3361 catcacgctt ggaattcatt aactccataa tcctgtttct gaggaaccat aactttgatg 3421 gactggatgt aagctggatc tacccagatc agaaagaaaa cactcatttc actgtgctga 3481 ttcatgagtt agcagaagcc tttcagaagg acttcacaaa atccaccaag gaaaggcttc 3541 tcttgactgt gggcgtatct gcagggaggc aaatgattga taacagctat caagttgaga 3601 aactggcaaa agatctggat ttcatcaacc tcctgtcctt tgacttccat gggtcttggg 3661 aaaagcccct tatcactggc cacaacagcc ctctgagcaa ggggtggcag gacagagggc 3721 caagctccta ctacaatgtg gaatatgctg tggggtactg gatacataag ggaatgccat 3781 cagagaaggt ggtcatgggc atccccacat atgggcactc cttcacactg gcctctgcag 3841 aaaccaccgt gggggcccct gcctctggcc ctggagctgc tggacccatc acagagtctt 3901 caggcttcct ggcctattat gagatctgcc agttcctgaa aggagccaag atcacgcggc 3961 tccaggatca gcaggttccc tacgcagtca aggggaacca gtgggtgggc tatgatgatg 4021 tgaagagtat ggagaccaag gttcagttct taaagaattt aaacctggga ggagccatga 4081 tctggtctat tgacatggat gacttcactg gcaaatcctg caaccagggc ccttaccctc 4141 ttgtccaagc agtcaagaga agccttggct ccctggaaaa cttgtacttt caaggctCCA 4201 GCTTTCTTGT ACAAAGTGGT ATGAGCAAGG GCGAGGAACT GTTCACCGGC GTGGTGCCCA 4261 TCCTGGTGGA ACTGGATGGC GACGTGAACG GCCACAAGTT CTCTGTGCGG GGAGAGGGCG 4321 AAGGCGACGC CACAAATGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 4381 CCGTGCCTTG GCCTACCCTC GTGACCACAC TGACCTACGG CGTGCAGTGC TTCAGCAGAT 4441 ACCCCGACCA TATGAAGCGG CACGACTTCT TCAAGAGCGC CATGCCCGAG GGCTACGTGC 4501 AGGAACGGAC CATCAGCTTC AAGGACGACG GCACCTACAA GACCAGAGCC GAAGTGAAGT 4561 TCGAGGGCGA CACCCTCGTG AACCGGATCG AGCTGAAGGG CATCGATTTC AAAGAGGACG 4621 GCAACATCCT GGGCCACAAG CTGGAGTACA ACTTCAACAG CCACAACGTG TACATCACCG 4681 CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC GTGGAAGATG 4741 GCAGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGAGAT GGCCCCGTGC 4801 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCGTGCT GAGCAAGGAC CCCAACGAGA 4861 AGCGGGACCA CATGGTGCTG CTGGAATTCG TGACCGCCGC TGGCATCACC CACGGCATGA 4921 GCGGCCTGAA CGACATCTTC GAGGCCCAGA AAATCGAGTG GCACGAGCAC CACCATCATC 4981 ACCATCACCA CTGATGATTC GAAAAATAAT AAGGATCCAA TCAACCTCTG GATTACAAAA 5041 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 5101 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 5161 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 5221 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 5281 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 5341 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 5401 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 5461 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 5521 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC 5581 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 5641 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 5701 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 5761 CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG 5821 AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG 5881 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA 5941 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC 6001 TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG 6061 TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 6121 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 6181 GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 6241 GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 6301 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 6361 TTTTAACAAA ATATTAACGC TTACAATTTA G //