LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2093..2189) /label=attL2 /note="attL2 (c)" misc_signal complement(2204..2223) /note="T7 Promoter/priming site (c)" primer_bind 2231..2247 /note="M13 Reverse priming site" gene 2360..3169 /note="Kanamycin resistance gene" rep_origin 3290..3963 /note="pUC origin" PCR_frame 669..2093 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2113) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2110) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2101..3966,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2071..2091 /note="TEV site" source 673..2070 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:125322 IMAGE:40005693" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: GATAGAC" gene 673..2070 /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /db_xref="GeneID:1118" /db_xref="HGNC:1936" /db_xref="MIM:600031" CDS 673..2070 /dnas_title="chitinase 1 (chitotriosidase)" /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /codon_start=1 /product="chitinase 1 (chitotriosidase)" /protein_id="AAI03696.1" /db_xref="GI:73909055" /db_xref="GeneID:1118" /db_xref="HGNC:1936" /db_xref="MIM:600031" /translation="MVRSVAWAGFMVLLMIPWGSAAKLVCYFTNWAQYRQGEARFLPK DLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFS TQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQ DLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSW EKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASS SDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGF DDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPS GTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAGGRLFQQSC PTGLVFSNSCKCCTWN" misc_difference 976..976 /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'S' in cDNA, 'G' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2070 /note="CHIT1" misc_difference 1997..1997 /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'G' in cDNA, 'A' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggtgcg gtctgtggcc tgggcaggtt tcatggtcct gctgatgatc 721 ccatggggct ctgctgcaaa actggtctgc tacttcacca actgggccca gtacagacag 781 ggggaggctc gcttcctgcc caaggacttg gaccccagcc tttgcaccca cctcatctac 841 gccttcgctg gcatgaccaa ccaccagctg agcaccactg agtggaatga cgagactctc 901 taccaggagt tcaatggcct gaagaagatg aatcccaagc tgaagaccct gttagccatc 961 ggaggctgga atttcagcac tcagaagttc acagatatgg tagccacggc caacaaccgt 1021 cagacctttg tcaactcggc catcaggttt ctgcgcaaat acagctttga cggccttgac 1081 cttgactggg agtacccagg aagccagggg agccctgccg tagacaagga gcgcttcaca 1141 accctggtac aggacttggc caatgccttc cagcaggaag cccagacctc agggaaggaa 1201 cgccttcttc tgagtgcagc ggttccagct gggcagacct atgtggatgc tggatacgag 1261 gtggacaaaa tcgcccagaa cctggatttt gtcaacctta tggcctacga cttccatggc 1321 tcttgggaga aggtcacggg acataacagc cccctctaca agaggcaaga agagagtggt 1381 gcagcagcca gcctcaacgt ggatgctgct gtgcaacagt ggctgcagaa ggggacccct 1441 gccagcaagc tgatccttgg catgcctacc tacggacgct ccttcacact ggcctcctca 1501 tcagacacca gagtgggggc cccagccaca gggtctggca ctccaggccc cttcaccaag 1561 gaaggaggga tgctggccta ctatgaagtc tgctcctgga agggggccac caaacagaga 1621 atccaggatc agaaggtgcc ctacatcttc cgggacaacc agtgggtggg ctttgatgat 1681 gtggagagct tcaaaaccaa ggtcagctat ctgaagcaga agggactggg cggggccatg 1741 gtctgggcac tggacttaga tgactttgcc ggcttctcct gcaaccaggg ccgatacccc 1801 ctcatccaga cgctacggca ggaactgagt cttccatact tgccttcagg caccccagag 1861 cttgaagttc caaaaccagg tcagccctct gaacctgagc atggccccag ccctggacaa 1921 gacacgttct gccagggcaa agctgatggg ctctatccca atcctcggga acggtccagc 1981 ttctacagct gtgcaggggg gcggctgttc cagcaaagct gcccgacagg cctggtgttc 2041 agcaactcct gcaaatgctg cacctggaat gaaaacttgt actttcaagg ctCCAGCTTT 2101 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 2161 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 2221 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 2281 TTACATTGCA CAAGATAAAA TAATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 2341 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 2401 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 2461 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 2521 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 2581 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 2641 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 2701 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 2761 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 2821 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 2881 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 2941 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 3001 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 3061 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 3121 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 3181 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 3241 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 3301 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 3361 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 3421 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA 3481 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 3541 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 3601 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 3661 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 3721 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 3781 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 3841 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 3901 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 3961 CATGTT //