LOCUS CHIT1-pGEn1-DEST 5914 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4542..5083 /note="WPRE" misc_recomb 4504..4526 /label=attB2 /note="attB2 site" misc_feature complement(5090..5304) /note="BGH pA" misc_feature complement(5392..5521) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4503 /dnas_title="CHIT1-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:125322 IMAGE:40005693" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: GATAGAC" gene 3166..4503 /dnas_title="CHIT1" /gene="CHIT1" /gene_synonym="CHI3" /gene_synonym="CHIT" /gene_synonym="FLJ00314" /db_xref="GeneID:1118" /db_xref="HGNC:1936" /db_xref="MIM:600031" CDS 3166..4503 /dnas_title="chitinase 1 (chitotriosidase)" /gene="CHIT1" /gene_synonym="CHI3" /gene_synonym="CHIT" /gene_synonym="FLJ00314" /codon_start=1 /product="chitinase 1 (chitotriosidase)" /protein_id="AAI03696.1" /db_xref="GI:73909055" /db_xref="GeneID:1118" /db_xref="HGNC:1936" /db_xref="MIM:600031" /translation="MVRSVAWAGFMVLLMIPWGSAAKLVCYFTNWAQYRQGEARFLPK DLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFS TQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQ DLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSW EKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASS SDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGF DDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPS GTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAGGRLFQQSC PTGLVFSNSCKCCTWN" misc_difference 3406..3406 /dnas_title="CHIT1" /gene="CHIT1" /gene_synonym="CHI3" /gene_synonym="CHIT" /gene_synonym="FLJ00314" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'S' in cDNA, 'G' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3166..4503 /note="CHIT1 coding region" misc_difference 4427..4427 /dnas_title="CHIT1" /gene="CHIT1" /gene_synonym="CHI3" /gene_synonym="CHIT" /gene_synonym="FLJ00314" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'G' in cDNA, 'A' in the human genome." vector join(4512..5914,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4511 /source="CHIT1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgcaaa actggtctgc 3181 tacttcacca actgggccca gtacagacag ggggaggctc gcttcctgcc caaggacttg 3241 gaccccagcc tttgcaccca cctcatctac gccttcgctg gcatgaccaa ccaccagctg 3301 agcaccactg agtggaatga cgagactctc taccaggagt tcaatggcct gaagaagatg 3361 aatcccaagc tgaagaccct gttagccatc ggaggctgga atttcagcac tcagaagttc 3421 acagatatgg tagccacggc caacaaccgt cagacctttg tcaactcggc catcaggttt 3481 ctgcgcaaat acagctttga cggccttgac cttgactggg agtacccagg aagccagggg 3541 agccctgccg tagacaagga gcgcttcaca accctggtac aggacttggc caatgccttc 3601 cagcaggaag cccagacctc agggaaggaa cgccttcttc tgagtgcagc ggttccagct 3661 gggcagacct atgtggatgc tggatacgag gtggacaaaa tcgcccagaa cctggatttt 3721 gtcaacctta tggcctacga cttccatggc tcttgggaga aggtcacggg acataacagc 3781 cccctctaca agaggcaaga agagagtggt gcagcagcca gcctcaacgt ggatgctgct 3841 gtgcaacagt ggctgcagaa ggggacccct gccagcaagc tgatccttgg catgcctacc 3901 tacggacgct ccttcacact ggcctcctca tcagacacca gagtgggggc cccagccaca 3961 gggtctggca ctccaggccc cttcaccaag gaaggaggga tgctggccta ctatgaagtc 4021 tgctcctgga agggggccac caaacagaga atccaggatc agaaggtgcc ctacatcttc 4081 cgggacaacc agtgggtggg ctttgatgat gtggagagct tcaaaaccaa ggtcagctat 4141 ctgaagcaga agggactggg cggggccatg gtctgggcac tggacttaga tgactttgcc 4201 ggcttctcct gcaaccaggg ccgatacccc ctcatccaga cgctacggca ggaactgagt 4261 cttccatact tgccttcagg caccccagag cttgaagttc caaaaccagg tcagccctct 4321 gaacctgagc atggccccag ccctggacaa gacacgttct gccagggcaa agctgatggg 4381 ctctatccca atcctcggga acggtccagc ttctacagct gtgcaggggg gcggctgttc 4441 cagcaaagct gcccgacagg cctggtgttc agcaactcct gcaaatgctg cacctggaat 4501 tgaCCAGCTT TCTTGTACAA AGTGGTTGAT AGCTTGGATC CAATCAACCT CTGGATTACA 4561 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 4621 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 4681 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 4741 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 4801 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 4861 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 4921 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 4981 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 5041 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT 5101 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 5161 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 5221 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 5281 AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC 5341 TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC 5401 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 5461 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 5521 GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA 5581 TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 5641 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 5701 ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 5761 TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 5821 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 5881 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG //