LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2030..2126) /label=attL2 /note="attL2 (c)" misc_signal complement(2141..2160) /note="T7 Promoter/priming site (c)" primer_bind 2168..2184 /note="M13 Reverse priming site" gene 2297..3106 /note="Kanamycin resistance gene" rep_origin 3227..3900 /note="pUC origin" vector join(2038..3903,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2029 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:125322 IMAGE:40005693" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: GATAGAC" gene 692..2029 /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /db_xref="GeneID:1118" /db_xref="HGNC:1936" /db_xref="MIM:600031" CDS 692..2029 /dnas_title="chitinase 1 (chitotriosidase)" /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /codon_start=1 /product="chitinase 1 (chitotriosidase)" /protein_id="AAI03696.1" /db_xref="GI:73909055" /db_xref="GeneID:1118" /db_xref="HGNC:1936" /db_xref="MIM:600031" /translation="MVRSVAWAGFMVLLMIPWGSAAKLVCYFTNWAQYRQGEARFLPK DLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFS TQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQ DLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSW EKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASS SDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGF DDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPS GTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAGGRLFQQSC PTGLVFSNSCKCCTWN" misc_difference 932..932 /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'S' in cDNA, 'G' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..2029 /note="CHIT1 coding region" misc_difference 1953..1953 /gene="CHIT1" /gene_synonym=CHI3 /gene_synonym=CHIT /gene_synonym=FLJ00314 /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'G' in cDNA, 'A' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgcaaaactg gtctgctact tcaccaactg 721 ggcccagtac agacaggggg aggctcgctt cctgcccaag gacttggacc ccagcctttg 781 cacccacctc atctacgcct tcgctggcat gaccaaccac cagctgagca ccactgagtg 841 gaatgacgag actctctacc aggagttcaa tggcctgaag aagatgaatc ccaagctgaa 901 gaccctgtta gccatcggag gctggaattt cagcactcag aagttcacag atatggtagc 961 cacggccaac aaccgtcaga cctttgtcaa ctcggccatc aggtttctgc gcaaatacag 1021 ctttgacggc cttgaccttg actgggagta cccaggaagc caggggagcc ctgccgtaga 1081 caaggagcgc ttcacaaccc tggtacagga cttggccaat gccttccagc aggaagccca 1141 gacctcaggg aaggaacgcc ttcttctgag tgcagcggtt ccagctgggc agacctatgt 1201 ggatgctgga tacgaggtgg acaaaatcgc ccagaacctg gattttgtca accttatggc 1261 ctacgacttc catggctctt gggagaaggt cacgggacat aacagccccc tctacaagag 1321 gcaagaagag agtggtgcag cagccagcct caacgtggat gctgctgtgc aacagtggct 1381 gcagaagggg acccctgcca gcaagctgat ccttggcatg cctacctacg gacgctcctt 1441 cacactggcc tcctcatcag acaccagagt gggggcccca gccacagggt ctggcactcc 1501 aggccccttc accaaggaag gagggatgct ggcctactat gaagtctgct cctggaaggg 1561 ggccaccaaa cagagaatcc aggatcagaa ggtgccctac atcttccggg acaaccagtg 1621 ggtgggcttt gatgatgtgg agagcttcaa aaccaaggtc agctatctga agcagaaggg 1681 actgggcggg gccatggtct gggcactgga cttagatgac tttgccggct tctcctgcaa 1741 ccagggccga taccccctca tccagacgct acggcaggaa ctgagtcttc catacttgcc 1801 ttcaggcacc ccagagcttg aagttccaaa accaggtcag ccctctgaac ctgagcatgg 1861 ccccagccct ggacaagaca cgttctgcca gggcaaagct gatgggctct atcccaatcc 1921 tcgggaacgg tccagcttct acagctgtgc aggggggcgg ctgttccagc aaagctgccc 1981 gacaggcctg gtgttcagca actcctgcaa atgctgcacc tggaattgaC CAGCTTTCTT 2041 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 2101 TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA 2161 TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA 2221 CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG 2281 TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC 2341 CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG 2401 TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG 2461 CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA 2521 ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT 2581 CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG 2641 TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG 2701 TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA 2761 TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA 2821 AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG 2881 TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT 2941 TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG 3001 TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA 3061 TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA 3121 TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC 3181 AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 3241 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 3301 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 3361 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT 3421 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 3481 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 3541 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 3601 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC 3661 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 3721 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 3781 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 3841 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 3901 GTT //