LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1850..1946) /label=attL2 /note="attL2 (c)" misc_signal complement(1961..1980) /note="T7 Promoter/priming site (c)" primer_bind 1988..2004 /note="M13 Reverse priming site" gene 2117..2926 /note="Kanamycin resistance gene" rep_origin 3047..3720 /note="pUC origin" PCR_frame 669..1850 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1870) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1867) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1858..3723,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1828..1848 /note="TEV site" source 673..1827 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:161611 IMAGE:8992049" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AATCATAG" gene 673..1827 /gene="CTBS" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" misc_feature 673..1827 /gene="CTBS" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 673..1827 /dnas_title="chitobiase, di-N-acetyl-" /gene="CTBS" /codon_start=1 /product="chitobiase, di-N-acetyl-" /protein_id="AAI26334.1" /db_xref="GI:116497033" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" /translation="MSRPQLRRWRLVSSPPSGVPGLALLALLALLALRLAAGTDCPCP EPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARV VLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTALV KETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSE CIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPF RGAPCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQ SISLKATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR" misc_feature 673..1827 /note="CTBS coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgtcccg gccgcagctt cgacgctggc gcctcgtctc tagcccgccg 721 agcggcgtcc cgggtctagc gctgctggcg ctgctggcgc tgctggcgct gcggctcgcg 781 gccgggaccg actgcccatg cccggagcct gagctctgcc gcccgattcg ccaccatcca 841 gatttcgagg tctttgtgtt tgatgttgga cagaaaactt ggaaatctta tgattggtca 901 cagattacaa ctgtggcaac atttggaaaa tatgactcag aacttatgtg ctacgctcat 961 tcaaaaggag ccagagtagt acttaaagga gatgtatcct taaaggatat cattgatcct 1021 gctttcagag catcctggat agctcaaaaa cttaatttgg ccaaaacaca atatatggat 1081 ggaattaata tagatataga gcaagaagtt aattgtttat cacctgaata tgatgcatta 1141 actgctttag tcaaagaaac tacagactct ttccatcgtg aaattgaggg atcacaggta 1201 acctttgatg tagcttggtc tccaaagaac atagacagaa gatgctataa ttatactgga 1261 atcgcagatg cttgtgactt cctctttgtg atgtcttatg atgaacaaag tcagatctgg 1321 tcagaatgta ttgcagcagc caatgctccc tataatcaga cattaactgg atataatgac 1381 tacatcaaga tgagcattaa tcctaagaaa cttgtaatgg gtgttccttg gtatggttat 1441 gattatacct gcctgaatct gtctgaggat catgtttgta ccattgcaaa agtccctttc 1501 cggggggctc cttgtagtga cgctgcagga cgtcaggtgc cctacaaaac gatcatgaag 1561 caaataaata gttctatttc tggaaaccta tgggataaag atcagcgggc tccttattat 1621 aactataaag atcctgctgg ccactttcat caagtatggt atgataaccc tcagagtatt 1681 tctttaaagg caacatatat acaaaactat cgcttacggg gcattggcat gtggaatgca 1741 aactgtcttg actactctgg agatgctgta gccaaacagc aaactgaaga aatgtgggaa 1801 gtcttaaagc caaagctgtt acagagagaa aacttgtact ttcaaggctC CAGCTTTCTT 1861 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 1921 TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA 1981 TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA 2041 CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG 2101 TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC 2161 CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG 2221 TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG 2281 CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA 2341 ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT 2401 CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG 2461 TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG 2521 TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA 2581 TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA 2641 AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG 2701 TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT 2761 TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG 2821 TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA 2881 TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA 2941 TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC 3001 AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 3061 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 3121 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 3181 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT 3241 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 3301 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 3361 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 3421 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC 3481 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 3541 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 3601 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 3661 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 3721 GTT //