LOCUS CTBS-pGEc1-DEST 5650 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5128..5257) /note="3'-ITR" misc_feature complement(4826..5040) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4278..4819 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4183..4205 /label=attB2 /note="attB2 site" misc_feature 4215..4238 /note="8xHis Tag" misc_feature 4242..4265 /note="StrepTag" PCR_frame 3002..4183 /note="Reading Frame" /set="Set 1" source 3006..4160 /dnas_title="CTBS-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:161611 IMAGE:8992049" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AATCATAG" gene 3006..4160 /dnas_title="CTBS" /gene="CTBS" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" misc_feature 3006..4160 /dnas_title="CTBS" /gene="CTBS" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 3006..4160 /dnas_title="chitobiase, di-N-acetyl-" /gene="CTBS" /codon_start=1 /product="chitobiase, di-N-acetyl-" /protein_id="AAI26334.1" /db_xref="GI:116497033" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" /translation="MSRPQLRRWRLVSSPPSGVPGLALLALLALLALRLAAGTDCPCP EPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARV VLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTALV KETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSE CIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPF RGAPCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQ SISLKATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR" misc_feature 3006..4160 /note="CTBS coding region" misc_feature 4161..4181 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4190) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4190) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4191..5650,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4190 /source="CTBS-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgtc ccggccgcag cttcgacgct ggcgcctcgt ctctagcccg ccgagcggcg 3061 tcccgggtct agcgctgctg gcgctgctgg cgctgctggc gctgcggctc gcggccggga 3121 ccgactgccc atgcccggag cctgagctct gccgcccgat tcgccaccat ccagatttcg 3181 aggtctttgt gtttgatgtt ggacagaaaa cttggaaatc ttatgattgg tcacagatta 3241 caactgtggc aacatttgga aaatatgact cagaacttat gtgctacgct cattcaaaag 3301 gagccagagt agtacttaaa ggagatgtat ccttaaagga tatcattgat cctgctttca 3361 gagcatcctg gatagctcaa aaacttaatt tggccaaaac acaatatatg gatggaatta 3421 atatagatat agagcaagaa gttaattgtt tatcacctga atatgatgca ttaactgctt 3481 tagtcaaaga aactacagac tctttccatc gtgaaattga gggatcacag gtaacctttg 3541 atgtagcttg gtctccaaag aacatagaca gaagatgcta taattatact ggaatcgcag 3601 atgcttgtga cttcctcttt gtgatgtctt atgatgaaca aagtcagatc tggtcagaat 3661 gtattgcagc agccaatgct ccctataatc agacattaac tggatataat gactacatca 3721 agatgagcat taatcctaag aaacttgtaa tgggtgttcc ttggtatggt tatgattata 3781 cctgcctgaa tctgtctgag gatcatgttt gtaccattgc aaaagtccct ttccgggggg 3841 ctccttgtag tgacgctgca ggacgtcagg tgccctacaa aacgatcatg aagcaaataa 3901 atagttctat ttctggaaac ctatgggata aagatcagcg ggctccttat tataactata 3961 aagatcctgc tggccacttt catcaagtat ggtatgataa ccctcagagt atttctttaa 4021 aggcaacata tatacaaaac tatcgcttac ggggcattgg catgtggaat gcaaactgtc 4081 ttgactactc tggagatgct gtagccaaac agcaaactga agaaatgtgg gaagtcttaa 4141 agccaaagct gttacagaga gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA 4201 GTGGTGATGG CCGCCATCAT CATCATCATC ATCATCATGC GTGGAGCCAC CCGCAGTTCG 4261 AAAAATAATA AGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 4321 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 4381 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 4441 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 4501 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 4561 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 4621 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT 4681 CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 4741 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 4801 GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 4861 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 4921 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 4981 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 5041 CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT 5101 AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT 5161 CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT 5221 TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC 5281 GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT 5341 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC 5401 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA 5461 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT 5521 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 5581 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 5641 TACAATTTAG //