LOCUS CTBS-pGEc2-DEST 6376 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5854..5983) /note="3'-ITR" misc_feature complement(5552..5766) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5004..5545 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4183..4205 /note="attB2" misc_feature 4983..4988 /note="BstB1 site" misc_feature 4206..4901 /note="Super GFP" misc_feature 4902..4952 /note="AviTag" misc_feature 4953..4976 /note="His Tag" PCR_frame 3002..4183 /note="Reading Frame" /set="Set 1" source 3006..4160 /dnas_title="CTBS-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:161611 IMAGE:8992049" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AATCATAG" gene 3006..4160 /dnas_title="CTBS" /gene="CTBS" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" misc_feature 3006..4160 /dnas_title="CTBS" /gene="CTBS" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 3006..4160 /dnas_title="chitobiase, di-N-acetyl-" /gene="CTBS" /codon_start=1 /product="chitobiase, di-N-acetyl-" /protein_id="AAI26334.1" /db_xref="GI:116497033" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" /translation="MSRPQLRRWRLVSSPPSGVPGLALLALLALLALRLAAGTDCPCP EPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARV VLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTALV KETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSE CIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPF RGAPCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQ SISLKATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR" misc_feature 3006..4160 /note="CTBS coding region" misc_feature 4161..4181 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4190) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4190) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4191..6376,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4190 /source="CTBS-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgtc ccggccgcag cttcgacgct ggcgcctcgt ctctagcccg ccgagcggcg 3061 tcccgggtct agcgctgctg gcgctgctgg cgctgctggc gctgcggctc gcggccggga 3121 ccgactgccc atgcccggag cctgagctct gccgcccgat tcgccaccat ccagatttcg 3181 aggtctttgt gtttgatgtt ggacagaaaa cttggaaatc ttatgattgg tcacagatta 3241 caactgtggc aacatttgga aaatatgact cagaacttat gtgctacgct cattcaaaag 3301 gagccagagt agtacttaaa ggagatgtat ccttaaagga tatcattgat cctgctttca 3361 gagcatcctg gatagctcaa aaacttaatt tggccaaaac acaatatatg gatggaatta 3421 atatagatat agagcaagaa gttaattgtt tatcacctga atatgatgca ttaactgctt 3481 tagtcaaaga aactacagac tctttccatc gtgaaattga gggatcacag gtaacctttg 3541 atgtagcttg gtctccaaag aacatagaca gaagatgcta taattatact ggaatcgcag 3601 atgcttgtga cttcctcttt gtgatgtctt atgatgaaca aagtcagatc tggtcagaat 3661 gtattgcagc agccaatgct ccctataatc agacattaac tggatataat gactacatca 3721 agatgagcat taatcctaag aaacttgtaa tgggtgttcc ttggtatggt tatgattata 3781 cctgcctgaa tctgtctgag gatcatgttt gtaccattgc aaaagtccct ttccgggggg 3841 ctccttgtag tgacgctgca ggacgtcagg tgccctacaa aacgatcatg aagcaaataa 3901 atagttctat ttctggaaac ctatgggata aagatcagcg ggctccttat tataactata 3961 aagatcctgc tggccacttt catcaagtat ggtatgataa ccctcagagt atttctttaa 4021 aggcaacata tatacaaaac tatcgcttac ggggcattgg catgtggaat gcaaactgtc 4081 ttgactactc tggagatgct gtagccaaac agcaaactga agaaatgtgg gaagtcttaa 4141 agccaaagct gttacagaga gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA 4201 GTGGTATGAG CAAGGGCGAG GAACTGTTCA CCGGCGTGGT GCCCATCCTG GTGGAACTGG 4261 ATGGCGACGT GAACGGCCAC AAGTTCTCTG TGCGGGGAGA GGGCGAAGGC GACGCCACAA 4321 ATGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCTTGGCCTA 4381 CCCTCGTGAC CACACTGACC TACGGCGTGC AGTGCTTCAG CAGATACCCC GACCATATGA 4441 AGCGGCACGA CTTCTTCAAG AGCGCCATGC CCGAGGGCTA CGTGCAGGAA CGGACCATCA 4501 GCTTCAAGGA CGACGGCACC TACAAGACCA GAGCCGAAGT GAAGTTCGAG GGCGACACCC 4561 TCGTGAACCG GATCGAGCTG AAGGGCATCG ATTTCAAAGA GGACGGCAAC ATCCTGGGCC 4621 ACAAGCTGGA GTACAACTTC AACAGCCACA ACGTGTACAT CACCGCCGAC AAGCAGAAGA 4681 ACGGCATCAA GGCCAACTTC AAGATCCGGC ACAACGTGGA AGATGGCAGC GTGCAGCTGG 4741 CCGACCACTA CCAGCAGAAC ACCCCCATCG GAGATGGCCC CGTGCTGCTG CCCGACAACC 4801 ACTACCTGAG CACCCAGAGC GTGCTGAGCA AGGACCCCAA CGAGAAGCGG GACCACATGG 4861 TGCTGCTGGA ATTCGTGACC GCCGCTGGCA TCACCCACGG CATGAGCGGC CTGAACGACA 4921 TCTTCGAGGC CCAGAAAATC GAGTGGCACG AGCACCACCA TCATCACCAT CACCACTGAT 4981 GATTCGAAAA ATAATAAGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 5041 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 5101 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 5161 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 5221 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 5281 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 5341 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC 5401 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 5461 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 5521 CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC 5581 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 5641 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 5701 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 5761 TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG 5821 ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA 5881 CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC 5941 CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA 6001 TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT 6061 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 6121 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 6181 CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG 6241 GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 6301 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 6361 AACGCTTACA ATTTAG //