LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1736..1832) /label=attL2 /note="attL2 (c)" misc_signal complement(1847..1866) /note="T7 Promoter/priming site (c)" primer_bind 1874..1890 /note="M13 Reverse priming site" gene 2003..2812 /note="Kanamycin resistance gene" rep_origin 2933..3606 /note="pUC origin" vector join(1744..3609,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1735 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:161611 IMAGE:8992049" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: AATCATAG" gene 692..1735 /gene="CTBS" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" misc_feature 692..1735 /gene="CTBS" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..1735 /dnas_title="chitobiase, di-N-acetyl-" /gene="CTBS" /codon_start=1 /product="chitobiase, di-N-acetyl-" /protein_id="AAI26334.1" /db_xref="GI:116497033" /db_xref="GeneID:1486" /db_xref="HGNC:2496" /db_xref="MIM:600873" /translation="MSRPQLRRWRLVSSPPSGVPGLALLALLALLALRLAAGTDCPCP EPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARV VLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTALV KETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSE CIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPF RGAPCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQ SISLKATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR" misc_feature 692..1735 /note="CTBS coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caccgactgc ccatgcccgg agcctgagct 721 ctgccgcccg attcgccacc atccagattt cgaggtcttt gtgtttgatg ttggacagaa 781 aacttggaaa tcttatgatt ggtcacagat tacaactgtg gcaacatttg gaaaatatga 841 ctcagaactt atgtgctacg ctcattcaaa aggagccaga gtagtactta aaggagatgt 901 atccttaaag gatatcattg atcctgcttt cagagcatcc tggatagctc aaaaacttaa 961 tttggccaaa acacaatata tggatggaat taatatagat atagagcaag aagttaattg 1021 tttatcacct gaatatgatg cattaactgc tttagtcaaa gaaactacag actctttcca 1081 tcgtgaaatt gagggatcac aggtaacctt tgatgtagct tggtctccaa agaacataga 1141 cagaagatgc tataattata ctggaatcgc agatgcttgt gacttcctct ttgtgatgtc 1201 ttatgatgaa caaagtcaga tctggtcaga atgtattgca gcagccaatg ctccctataa 1261 tcagacatta actggatata atgactacat caagatgagc attaatccta agaaacttgt 1321 aatgggtgtt ccttggtatg gttatgatta tacctgcctg aatctgtctg aggatcatgt 1381 ttgtaccatt gcaaaagtcc ctttccgggg ggctccttgt agtgacgctg caggacgtca 1441 ggtgccctac aaaacgatca tgaagcaaat aaatagttct atttctggaa acctatggga 1501 taaagatcag cgggctcctt attataacta taaagatcct gctggccact ttcatcaagt 1561 atggtatgat aaccctcaga gtatttcttt aaaggcaaca tatatacaaa actatcgctt 1621 acggggcatt ggcatgtgga atgcaaactg tcttgactac tctggagatg ctgtagccaa 1681 acagcaaact gaagaaatgt gggaagtctt aaagccaaag ctgttacaga gatgaCCAGC 1741 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 1801 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 1861 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 1921 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 1981 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2041 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2101 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2161 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2221 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2281 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2341 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2401 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2461 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2521 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 2581 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 2641 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 2701 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 2761 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 2821 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 2881 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 2941 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3001 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3061 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3121 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3181 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3241 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3301 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3361 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3421 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3481 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3541 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 3601 TCACATGTT //