LOCUS OVGP1-pGEn2-DEST 7275 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5903..6444 /note="WPRE" misc_recomb 5865..5887 /label=attB2 /note="attB2 site" misc_feature complement(6451..6665) /note="BGH pA" misc_feature complement(6753..6882) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..5864 /dnas_title="OVGP1-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:161455 IMAGE:8991893" /tissue_type="Pooled, cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus, PCR rescued clones" /clone_lib="NIH_MGC_362" /lab_host="DH10B" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: TTCCGTTT" gene 3891..5864 /dnas_title="OVGP1" /gene="OVGP1" /gene_synonym="CHIT5" /gene_synonym="EGP" /gene_synonym="OGP" /db_xref="GeneID:5016" /db_xref="HGNC:8524" /db_xref="MIM:603578" misc_feature 3891..5864 /dnas_title="OVGP1" /gene="OVGP1" /gene_synonym="CHIT5" /gene_synonym="EGP" /gene_synonym="OGP" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 3891..5864 /dnas_title="oviductal glycoprotein 1, 120kDa" /gene="OVGP1" /gene_synonym="CHIT5" /gene_synonym="EGP" /gene_synonym="OGP" /codon_start=1 /product="oviductal glycoprotein 1, 120kDa" /protein_id="AAI26178.1" /db_xref="GI:116497123" /db_xref="GeneID:5016" /db_xref="HGNC:8524" /db_xref="MIM:603578" /translation="MWKLLLWVGLVLVLKHHDGAAHKLVCYFTNWAHSRPGPASILPH DLDPFLCTHLIFAFASMNNNQIVAKDLQDEKILYPEFNKLKERNRELKTLLSIGGWNF GTSRFTTMLSTFANREKFIASVISLLRTHDFDGLDLFFLYPGLRGSPMHDRWTFLFLI EELLFAFRKEALLTMRPRLLLSAAVSGVPHIVQTSYDVRFLGRLLDFINVLSYDLHGS WERFTGHNSPLFSLPEDPKSSAYAMNYWRKLGAPSEKLIMGIPTYGRTFRLLKASKNG LQARAIGPASPGKYTKQEGFLAYFEICSFVWGAKKHWIDYQYVPYANKGKEWVGYDNA ISFSYKAWFIRREHFGGAMVWTLDMDDVRGTFCGTGPFPLVYVLNDILVRAEFSSTSL PQFWLSSAVNSSSTDPERLAVTTAWTTDSKILPPGGEAGVTEIHGKCENMTITPRGTT VTPTKETVSLGKHTVALGEKTEITGAMTMTSVGHQSMTPGEKALTPVGHQSVTTGQKT LTSVGYQSVTPGEKTLTPVGHQSVTPVSHQSVSPGGTTMTPVHFQTETLRQNTVAPRR KAVAREKVTVPSRNISVTPEGQTMPLRGENLTSEVGTHPRMGNLGLQMEAENRMMLSS SPVIQLPEQTPLAFDNRFVPIYGNHSSVNSVTPQTSPLSLKKEIPENSAVDEEA" misc_feature 3891..5864 /note="OVGP1 coding region" vector join(5873..7275,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..5872 /source="OVGP1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc cataaactcg 3901 tgtgttattt caccaactgg gcacacagtc ggccaggccc tgcctcgatc ttgccccatg 3961 acctggaccc ctttctctgc acccacctga tatttgcctt tgcctcaatg aacaacaatc 4021 agattgttgc taaggatctc caggatgaga aaattctcta cccagagttc aacaaactaa 4081 aggagaggaa cagagagctg aaaacactac tgtccatcgg cgggtggaac tttggcacct 4141 caagattcac cactatgttg tccacatttg ccaaccgtga aaagtttatt gcttcagtta 4201 tatcccttct gaggacacat gactttgatg gtcttgacct tttcttctta tatcctggac 4261 taagaggcag ccccatgcat gaccggtgga cttttctctt cttaattgaa gagctcctgt 4321 ttgccttccg gaaggaggca ctgctcacca tgcgcccgag gctgctgctg tctgctgctg 4381 tttctggggt cccacacatc gtccaaacat cctatgatgt gcgctttcta ggaagactcc 4441 tggatttcat caatgtcttg tcttatgact tacatggaag ttgggaaagg ttcacaggac 4501 ataatagccc cctcttctct ctgcctgaag accccaaatc ttcggcatat gctatgaatt 4561 attggagaaa gcttggggca ccctcagaga agctcatcat ggggatcccc acctatggac 4621 gtacctttcg cctcctcaaa gcctctaaga atgggttgca ggccagagcg atcggaccag 4681 catctccagg gaagtacacc aagcaagaag gcttcttggc ttattttgag atttgttcct 4741 ttgtctgggg agcgaagaag cactggattg attaccagta tgtcccgtat gccaacaagg 4801 ggaaagagtg ggttggctat gacaatgcca tcagcttcag ttacaaggca tggtttataa 4861 ggcgagagca ttttgggggg gccatggtgt ggacattgga catggatgac gtcaggggca 4921 cgttctgtgg cactggccct ttcccccttg tctacgtatt gaatgatatc ctggtgcggg 4981 ctgagttcag ttcaacttct ttaccacaat tttggctgtc atctgctgtg aattcttcaa 5041 gcactgaccc tgaaaggctg gctgtgacca cggcatggac cactgatagt aagattttgc 5101 ccccaggagg agaggctggg gtcactgaga tccacggaaa gtgtgaaaat atgactataa 5161 cccctagagg tacaactgtg acccctacaa aggaaactgt atcccttgga aagcacactg 5221 tagctctagg agagaagact gagatcactg gggcaatgac catgacttct gtgggtcatc 5281 agtccatgac ccctggagag aaggccctga cccctgtggg tcatcaatct gtgaccactg 5341 gacagaagac cctgacctct gtgggttatc agtctgtgac ccctggggaa aagaccctga 5401 cccctgtggg tcatcagtct gtgacccctg tgagtcatca gtctgtgagc cctggaggaa 5461 cgactatgac ccctgtccat tttcagactg agacccttag acagaataca gtggccccta 5521 gaaggaaggc tgtggcccgt gaaaaggtga ctgtcccctc cagaaacata tcagtcaccc 5581 ctgaagggca gactatgcct ttaagagggg agaatttgac ttctgaggtg ggcactcacc 5641 ccaggatggg taacttgggt cttcagatgg aagctgaaaa caggatgatg ctgtcctcca 5701 gccccgtcat ccagctcccg gaacaaactc ctctagcttt tgacaaccgc tttgttccca 5761 tctatggaaa ccattcctct gtcaactcag taacccctca aacaagtcct ctttctctaa 5821 aaaaagaaat cccagaaaac tctgctgtgg atgaagaagc ctaaCCAGCT TTCTTGTACA 5881 AAGTGGTTGA TAGCTTGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 5941 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 6001 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 6061 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 6121 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 6181 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 6241 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 6301 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 6361 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 6421 TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 6481 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 6541 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 6601 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 6661 GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA 6721 TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC 6781 TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC 6841 GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT 6901 GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC 6961 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 7021 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 7081 CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG 7141 CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 7201 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA 7261 ACGCTTACAA TTTAG //