LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2648..2744) /label=attL2 /note="attL2 (c)" misc_signal complement(2759..2778) /note="T7 Promoter/priming site (c)" primer_bind 2786..2802 /note="M13 Reverse priming site" gene 2915..3724 /note="Kanamycin resistance gene" rep_origin 3845..4518 /note="pUC origin" PCR_frame 669..2648 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2668) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2665) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2656..4521,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2626..2646 /note="TEV site" source 673..2625 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:20659 IMAGE:4662011" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2625 /gene="GUSB" /gene_synonym=MPS7 /db_xref="GeneID:2990" /db_xref="HGNC:4696" /db_xref="MIM:253220" CDS 673..2625 /dnas_title="glucuronidase, beta" /gene="GUSB" /gene_synonym=MPS7 /codon_start=1 /product="glucuronidase, beta" /protein_id="AAH14142.1" /db_xref="GI:15559560" /db_xref="GeneID:2990" /db_xref="HGNC:4696" /db_xref="MIM:253220" /translation="MARGSAVAWAALGPLLWGCALGLQGGMLYPQESPSRECKELDGL WSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYE REVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVG PLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSV LLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGT QGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKS QFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEV MQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVAN EPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWY HDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLL EQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRE RYWKIANETRYPHSVAKSQCLENSLFT" misc_feature 673..2625 /note="GUSB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcccg ggggtcggcg gttgcctggg cggcgctcgg gccgttgttg 721 tggggctgcg cgctggggct gcagggcggg atgctgtacc cccaggagag cccgtcgcgg 781 gagtgcaagg agctggacgg cctctggagc ttccgcgccg acttctctga caaccgacgc 841 cggggcttcg aggagcagtg gtaccggcgg ccgctgtggg agtcaggccc caccgtggac 901 atgccagttc cctccagctt caatgacatc agccaggact ggcgtctgcg gcattttgtc 961 ggctgggtgt ggtacgaacg ggaggtgatc ctgccggagc gatggaccca ggacctgcgc 1021 acaagagtgg tgctgaggat tggcagtgcc cattcctatg ccatcgtgtg ggtgaatggg 1081 gtcgacacgc tagagcatga ggggggctac ctccccttcg aggccgacat cagcaacctg 1141 gtccaggtgg ggcccctgcc ctcccggctc cgaatcacta tcgccatcaa caacacactc 1201 acccccacca ccctgccacc agggaccatc caatacctga ctgacacctc caagtatccc 1261 aagggttact ttgtccagaa cacatatttt gactttttca actacgctgg actgcagcgg 1321 tctgtacttc tgtacacgac acccaccacc tacatcgatg acatcaccgt caccaccagc 1381 gtggagcaag acagtgggct ggtgaattac cagatctctg tcaagggcag taacctgttc 1441 aagttggaag tgcgtctttt ggatgcagaa aacaaagtcg tggcgaatgg gactgggacc 1501 cagggccaac ttaaggtgcc aggtgtcagc ctctggtggc cgtacctgat gcacgaacgc 1561 cctgcctatc tgtattcatt ggaggtgcag ctgactgcac agacgtcact ggggcctgtg 1621 tctgacttct acacactccc tgtggggatc cgcactgtgg ctgtcaccaa gagccagttc 1681 ctcatcaatg ggaaaccttt ctatttccac ggtgtcaaca agcatgagga tgcggacatc 1741 cgagggaagg gcttcgactg gccgctgctg gtgaaggact tcaacctgct tcgctggctt 1801 ggtgccaacg ctttccgtac cagccactac ccctatgcag aggaagtgat gcagatgtgt 1861 gaccgctatg ggattgtggt catcgatgag tgtcccggcg tgggcctggc gctgccgcag 1921 ttcttcaaca acgtttctct gcatcaccac atgcaggtga tggaagaagt ggtgcgtagg 1981 gacaagaacc accccgcggt cgtgatgtgg tctgtggcca acgagcctgc gtcccaccta 2041 gaatctgctg gctactactt gaagatggtg atcgctcaca ccaaatcctt ggacccctcc 2101 cggcctgtga cctttgtgag caactctaac tatgcagcag acaagggggc tccgtatgtg 2161 gatgtgatct gtttgaacag ctactactct tggtatcacg actacgggca cctggagttg 2221 attcagctgc agctggccac ccagtttgag aactggtata agaagtatca gaagcccatt 2281 attcagagcg agtatggagc agaaacgatt gcagggtttc accaggatcc acctctgatg 2341 ttcactgaag agtaccagaa aagtctgcta gagcagtacc atctgggtct ggatcaaaaa 2401 cgcagaaaat acgtggttgg agagctcatt tggaattttg ccgatttcat gactgaacag 2461 tcaccgacga gagtgctggg gaataaaaag gggatcttca ctcggcagag acaaccaaaa 2521 agtgcagcgt tccttttgcg agagagatac tggaagattg ccaatgaaac caggtatccc 2581 cactcagtag ccaagtcaca atgtttggaa aacagcctgt ttactgaaaa cttgtacttt 2641 caaggctCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG 2701 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT 2761 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC 2821 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA TCATCATGAA CAATAAAACT 2881 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC 2941 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 3001 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA 3061 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 3121 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 3181 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA 3241 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 3301 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 3361 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 3421 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA 3481 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 3541 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 3601 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 3661 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 3721 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA 3781 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC 3841 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 3901 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 3961 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 4021 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 4081 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 4141 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 4201 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 4261 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 4321 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 4381 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 4441 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 4501 CTGGCCTTTT GCTCACATGT T //