LOCUS GUSB-pGEc2-DEST 7174 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6652..6781) /note="3'-ITR" misc_feature complement(6350..6564) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5802..6343 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4981..5003 /note="attB2" misc_feature 5781..5786 /note="BstB1 site" misc_feature 5004..5699 /note="Super GFP" misc_feature 5700..5750 /note="AviTag" misc_feature 5751..5774 /note="His Tag" PCR_frame 3002..4981 /note="Reading Frame" /set="Set 1" source 3006..4958 /dnas_title="GUSB-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:20659 IMAGE:4662011" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4958 /dnas_title="GUSB" /gene="GUSB" /gene_synonym="MPS7" /db_xref="GeneID:2990" /db_xref="HGNC:4696" /db_xref="MIM:253220" CDS 3006..4958 /dnas_title="glucuronidase, beta" /gene="GUSB" /gene_synonym="MPS7" /codon_start=1 /product="glucuronidase, beta" /protein_id="AAH14142.1" /db_xref="GI:15559560" /db_xref="GeneID:2990" /db_xref="HGNC:4696" /db_xref="MIM:253220" /translation="MARGSAVAWAALGPLLWGCALGLQGGMLYPQESPSRECKELDGL WSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYE REVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVG PLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSV LLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGT QGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKS QFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEV MQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVAN EPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWY HDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLL EQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRE RYWKIANETRYPHSVAKSQCLENSLFT" misc_feature 3006..4958 /note="GUSB coding region" misc_feature 4959..4979 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4988) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4988) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4989..7174,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4988 /source="GUSB-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ccgggggtcg gcggttgcct gggcggcgct cgggccgttg ttgtggggct 3061 gcgcgctggg gctgcagggc gggatgctgt acccccagga gagcccgtcg cgggagtgca 3121 aggagctgga cggcctctgg agcttccgcg ccgacttctc tgacaaccga cgccggggct 3181 tcgaggagca gtggtaccgg cggccgctgt gggagtcagg ccccaccgtg gacatgccag 3241 ttccctccag cttcaatgac atcagccagg actggcgtct gcggcatttt gtcggctggg 3301 tgtggtacga acgggaggtg atcctgccgg agcgatggac ccaggacctg cgcacaagag 3361 tggtgctgag gattggcagt gcccattcct atgccatcgt gtgggtgaat ggggtcgaca 3421 cgctagagca tgaggggggc tacctcccct tcgaggccga catcagcaac ctggtccagg 3481 tggggcccct gccctcccgg ctccgaatca ctatcgccat caacaacaca ctcaccccca 3541 ccaccctgcc accagggacc atccaatacc tgactgacac ctccaagtat cccaagggtt 3601 actttgtcca gaacacatat tttgactttt tcaactacgc tggactgcag cggtctgtac 3661 ttctgtacac gacacccacc acctacatcg atgacatcac cgtcaccacc agcgtggagc 3721 aagacagtgg gctggtgaat taccagatct ctgtcaaggg cagtaacctg ttcaagttgg 3781 aagtgcgtct tttggatgca gaaaacaaag tcgtggcgaa tgggactggg acccagggcc 3841 aacttaaggt gccaggtgtc agcctctggt ggccgtacct gatgcacgaa cgccctgcct 3901 atctgtattc attggaggtg cagctgactg cacagacgtc actggggcct gtgtctgact 3961 tctacacact ccctgtgggg atccgcactg tggctgtcac caagagccag ttcctcatca 4021 atgggaaacc tttctatttc cacggtgtca acaagcatga ggatgcggac atccgaggga 4081 agggcttcga ctggccgctg ctggtgaagg acttcaacct gcttcgctgg cttggtgcca 4141 acgctttccg taccagccac tacccctatg cagaggaagt gatgcagatg tgtgaccgct 4201 atgggattgt ggtcatcgat gagtgtcccg gcgtgggcct ggcgctgccg cagttcttca 4261 acaacgtttc tctgcatcac cacatgcagg tgatggaaga agtggtgcgt agggacaaga 4321 accaccccgc ggtcgtgatg tggtctgtgg ccaacgagcc tgcgtcccac ctagaatctg 4381 ctggctacta cttgaagatg gtgatcgctc acaccaaatc cttggacccc tcccggcctg 4441 tgacctttgt gagcaactct aactatgcag cagacaaggg ggctccgtat gtggatgtga 4501 tctgtttgaa cagctactac tcttggtatc acgactacgg gcacctggag ttgattcagc 4561 tgcagctggc cacccagttt gagaactggt ataagaagta tcagaagccc attattcaga 4621 gcgagtatgg agcagaaacg attgcagggt ttcaccagga tccacctctg atgttcactg 4681 aagagtacca gaaaagtctg ctagagcagt accatctggg tctggatcaa aaacgcagaa 4741 aatacgtggt tggagagctc atttggaatt ttgccgattt catgactgaa cagtcaccga 4801 cgagagtgct ggggaataaa aaggggatct tcactcggca gagacaacca aaaagtgcag 4861 cgttcctttt gcgagagaga tactggaaga ttgccaatga aaccaggtat ccccactcag 4921 tagccaagtc acaatgtttg gaaaacagcc tgtttactga aaacttgtac tttcaaggct 4981 CCAGCTTTCT TGTACAAAGT GGTATGAGCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC 5041 CCATCCTGGT GGAACTGGAT GGCGACGTGA ACGGCCACAA GTTCTCTGTG CGGGGAGAGG 5101 GCGAAGGCGA CGCCACAAAT GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC 5161 TGCCCGTGCC TTGGCCTACC CTCGTGACCA CACTGACCTA CGGCGTGCAG TGCTTCAGCA 5221 GATACCCCGA CCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACG 5281 TGCAGGAACG GACCATCAGC TTCAAGGACG ACGGCACCTA CAAGACCAGA GCCGAAGTGA 5341 AGTTCGAGGG CGACACCCTC GTGAACCGGA TCGAGCTGAA GGGCATCGAT TTCAAAGAGG 5401 ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTGTACATCA 5461 CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGGCAC AACGTGGAAG 5521 ATGGCAGCGT GCAGCTGGCC GACCACTACC AGCAGAACAC CCCCATCGGA GATGGCCCCG 5581 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGAGCGT GCTGAGCAAG GACCCCAACG 5641 AGAAGCGGGA CCACATGGTG CTGCTGGAAT TCGTGACCGC CGCTGGCATC ACCCACGGCA 5701 TGAGCGGCCT GAACGACATC TTCGAGGCCC AGAAAATCGA GTGGCACGAG CACCACCATC 5761 ATCACCATCA CCACTGATGA TTCGAAAAAT AATAAGGATC CAATCAACCT CTGGATTACA 5821 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 5881 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 5941 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 6001 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 6061 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 6121 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 6181 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 6241 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 6301 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT 6361 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 6421 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 6481 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 6541 AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC 6601 TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC 6661 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 6721 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 6781 GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA 6841 TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 6901 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 6961 ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 7021 TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 7081 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 7141 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG //