LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2582..2678) /label=attL2 /note="attL2 (c)" misc_signal complement(2693..2712) /note="T7 Promoter/priming site (c)" primer_bind 2720..2736 /note="M13 Reverse priming site" gene 2849..3658 /note="Kanamycin resistance gene" rep_origin 3779..4452 /note="pUC origin" vector join(2590..4455,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2581 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:20659 IMAGE:4662011" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2581 /gene="GUSB" /gene_synonym=MPS7 /db_xref="GeneID:2990" /db_xref="HGNC:4696" /db_xref="MIM:253220" CDS 692..2581 /dnas_title="glucuronidase, beta" /gene="GUSB" /gene_synonym=MPS7 /codon_start=1 /product="glucuronidase, beta" /protein_id="AAH14142.1" /db_xref="GI:15559560" /db_xref="GeneID:2990" /db_xref="HGNC:4696" /db_xref="MIM:253220" /translation="MARGSAVAWAALGPLLWGCALGLQGGMLYPQESPSRECKELDGL WSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYE REVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVG PLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSV LLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGT QGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKS QFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEV MQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVAN EPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWY HDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLL EQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRE RYWKIANETRYPHSVAKSQCLENSLFT" misc_feature 692..2581 /note="GUSB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cctgcagggc gggatgctgt acccccagga 721 gagcccgtcg cgggagtgca aggagctgga cggcctctgg agcttccgcg ccgacttctc 781 tgacaaccga cgccggggct tcgaggagca gtggtaccgg cggccgctgt gggagtcagg 841 ccccaccgtg gacatgccag ttccctccag cttcaatgac atcagccagg actggcgtct 901 gcggcatttt gtcggctggg tgtggtacga acgggaggtg atcctgccgg agcgatggac 961 ccaggacctg cgcacaagag tggtgctgag gattggcagt gcccattcct atgccatcgt 1021 gtgggtgaat ggggtcgaca cgctagagca tgaggggggc tacctcccct tcgaggccga 1081 catcagcaac ctggtccagg tggggcccct gccctcccgg ctccgaatca ctatcgccat 1141 caacaacaca ctcaccccca ccaccctgcc accagggacc atccaatacc tgactgacac 1201 ctccaagtat cccaagggtt actttgtcca gaacacatat tttgactttt tcaactacgc 1261 tggactgcag cggtctgtac ttctgtacac gacacccacc acctacatcg atgacatcac 1321 cgtcaccacc agcgtggagc aagacagtgg gctggtgaat taccagatct ctgtcaaggg 1381 cagtaacctg ttcaagttgg aagtgcgtct tttggatgca gaaaacaaag tcgtggcgaa 1441 tgggactggg acccagggcc aacttaaggt gccaggtgtc agcctctggt ggccgtacct 1501 gatgcacgaa cgccctgcct atctgtattc attggaggtg cagctgactg cacagacgtc 1561 actggggcct gtgtctgact tctacacact ccctgtgggg atccgcactg tggctgtcac 1621 caagagccag ttcctcatca atgggaaacc tttctatttc cacggtgtca acaagcatga 1681 ggatgcggac atccgaggga agggcttcga ctggccgctg ctggtgaagg acttcaacct 1741 gcttcgctgg cttggtgcca acgctttccg taccagccac tacccctatg cagaggaagt 1801 gatgcagatg tgtgaccgct atgggattgt ggtcatcgat gagtgtcccg gcgtgggcct 1861 ggcgctgccg cagttcttca acaacgtttc tctgcatcac cacatgcagg tgatggaaga 1921 agtggtgcgt agggacaaga accaccccgc ggtcgtgatg tggtctgtgg ccaacgagcc 1981 tgcgtcccac ctagaatctg ctggctacta cttgaagatg gtgatcgctc acaccaaatc 2041 cttggacccc tcccggcctg tgacctttgt gagcaactct aactatgcag cagacaaggg 2101 ggctccgtat gtggatgtga tctgtttgaa cagctactac tcttggtatc acgactacgg 2161 gcacctggag ttgattcagc tgcagctggc cacccagttt gagaactggt ataagaagta 2221 tcagaagccc attattcaga gcgagtatgg agcagaaacg attgcagggt ttcaccagga 2281 tccacctctg atgttcactg aagagtacca gaaaagtctg ctagagcagt accatctggg 2341 tctggatcaa aaacgcagaa aatacgtggt tggagagctc atttggaatt ttgccgattt 2401 catgactgaa cagtcaccga cgagagtgct ggggaataaa aaggggatct tcactcggca 2461 gagacaacca aaaagtgcag cgttcctttt gcgagagaga tactggaaga ttgccaatga 2521 aaccaggtat ccccactcag tagccaagtc acaatgtttg gaaaacagcc tgtttacttg 2581 aCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2641 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2701 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2761 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2821 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2881 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2941 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 3001 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 3061 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 3121 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 3181 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 3241 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 3301 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 3361 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 3421 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3481 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3541 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3601 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3661 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3721 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3781 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3841 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3901 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3961 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 4021 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 4081 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 4141 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 4201 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 4261 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 4321 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 4381 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4441 TTTTGCTCAC ATGTT //