LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3281..3377) /label=attL2 /note="attL2 (c)" misc_signal complement(3392..3411) /note="T7 Promoter/priming site (c)" primer_bind 3419..3435 /note="M13 Reverse priming site" gene 3548..4357 /note="Kanamycin resistance gene" rep_origin 4478..5151 /note="pUC origin" vector join(3289..5154,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3280 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:23037 IMAGE:4905535" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..3280 /gene="MANBA" /gene_synonym=MANB1 /db_xref="GeneID:4126" /db_xref="HGNC:6831" /db_xref="MIM:609489" CDS 692..3280 /dnas_title="mannosidase, beta A, lysosomal" /gene="MANBA" /gene_synonym=MANB1 /codon_start=1 /product="mannosidase, beta A, lysosomal" /protein_id="AAH15743.1" /db_xref="GI:16041731" /db_xref="GeneID:4126" /db_xref="HGNC:6831" /db_xref="MIM:609489" /translation="MRLHLLLLLALCGAGTTAAELSYSLRGNWSICNGNGSLELPGAV PGCVHSALFQQGLIQDSYYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEG VDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDVNSIELRFQSAVLYAAQQSKAHTR YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYF TFSPIYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAIPKLQTQQTYSIELQPGKRIVE LFVNISKNITVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIK GSPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGG GIYEQDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVTAEVAYQIKRLKSHPSII IWSGNNENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPT NGAETVAEAWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTL EKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDTIYLTQV MQAQCVKTETEFYRRSRSEIVDQQGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHY FAQNFFAPLLPVGFENENMFYIYGVSDLHSDYSMTLSVRVHTWSSLEPVCSRVTERFV MKGGEAVCLYEEPVSELLRRCGNCTRESCVVSFYLSADHELLSPTNYHFLSSPKEAVG LCKAQITAIISQQGDIFVFDLETSAVAPFVWLDVGSIPGRFSDNGFLMTEKTRTILFY PWEPTSKNELEQSFHVTSLTDIY" misc_feature 692..3280 /note="MANB1" misc_difference 2742..2742 /gene="MANBA" /gene_synonym=MANB1 /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'M' in cDNA, 'T' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3008..3008 /gene="MANBA" /gene_synonym=MANB1 /note="'C' in cDNA is 'T' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgccgcggag ctcagttaca gcttgcgtgg 721 caactggagc atctgcaatg ggaacggctc gctggagctg cccggggcgg tccctggctg 781 cgtgcacagc gccttgttcc agcagggcct gatccaggat tcttactaca gatttaatga 841 ccttaactac agatgggtct ctttggataa ctggacctat agcaaagaat ttaaaatccc 901 ctttgaaatt agcaaatggc aaaaagtaaa tttgattctt gagggagtgg atacggtttc 961 aaaaatcctg ttcaatgaag tcactattgg ggaaacagac aatatgttca atagatatag 1021 ctttgatatt accaacgtgg tcagggacgt gaactccatt gagctgcgtt tccagtcagc 1081 ggtgttgtat gcagcacagc agagcaaagc tcacactcgc taccaggttc ccccagactg 1141 ccctccactt gtgcagaagg gtgaatgcca tgtcaacttt gttcggaagg agcaatgttc 1201 ctttagttgg gactgggggc cttcctttcc tacccaggga atctggaaag atgttagaat 1261 tgaagcctat aatatttgtc acctgaacta cttcacattt tccccaatat atgataagag 1321 tgcccaggag tggaatctgg aaatagagtc tacatttgat gttgtcagct caaagccagt 1381 tggtggtcaa gtgatcgtag ccatccctaa gttgcaaaca caacagacat acagcattga 1441 acttcaacct gggaaaagga ttgttgagct atttgtgaac attagcaaga atattactgt 1501 agaaacttgg tggcctcatg gacatggaaa ccagactggg tacaacatga ctgttctttt 1561 tgaactggat ggaggcttaa atattgaaaa atcagctaag gtttatttta ggacagtgga 1621 acttatagaa gagcctataa aagggtctcc tggtttgagt ttctatttca aaattaatgg 1681 atttcccata tttctaaaag gctcaaactg gatcccagca gattcattcc aggaccgagt 1741 aacctctgag ttgttacggc tccttttaca gtctgttgtg gatgctaata tgaatactct 1801 tcgggtttgg ggaggaggaa tttatgagca ggatgaattc tatgaactct gtgatgaact 1861 aggaataatg gtatggcagg attttatgtt tgcctgtgcc ctttatccaa ctgatcaggg 1921 cttcctggat tcagtgacag cagaagttgc ctaccagatc aagagactga aatctcatcc 1981 ttctatcatc atatggagtg gcaataatga aaatgaggag gcgctgatga tgaattggta 2041 tcatatcagt ttcactgacc ggccaatcta catcaaggac tatgtgacac tctatgtgaa 2101 aaacatcaga gagctcgtac tggcaggaga caagagtcgt ccttttatta cgtccagtcc 2161 tacaaatggg gctgaaactg ttgcagaagc ctgggtctct caaaacccta atagcaatta 2221 ttttggtgat gtacattttt atgactatat cagtgattgc tggaactgga aagttttccc 2281 aaaagctcga tttgcatctg aatatggata tcagtcctgg ccgtccttca gtacattaga 2341 aaaggtctcg tctacagagg actggtcttt caatagcaag ttttcacttc atcgacaaca 2401 tcacgaaggt ggtaacaaac aaatgcttta tcaggctgga cttcatttca aactccccca 2461 aagcacagat ccattacgca catttaaaga taccatctac cttactcagg tgatgcaggc 2521 ccagtgtgtc aaaacagaaa ctgaattcta ccgccgtagt cgcagcgaga tagtggatca 2581 gcaagggcac acgatggggg cactttattg gcagttgaat gacatctggc aagctccttc 2641 ctgggcttct cttgagtacg gaggaaagtg gaaaatgctt cattactttg ctcagaattt 2701 ctttgctcca ctgttgccag taggctttga gaatgaaaac atgttctata tctatggtgt 2761 gtcagatctt cactcggatt attcgatgac actcagtgtg agagtccata catggagctc 2821 cctggagccc gtgtgctctc gtgtgactga acgttttgtg atgaaaggag gagaggctgt 2881 ctgcctttat gaggagccag tgtctgaatt gctgaggaga tgtgggaatt gcacacggga 2941 aagctgtgtg gtttcctttt acctttcagc tgaccatgaa ctcctgagcc cgaccaacta 3001 ccacttcctg tcctcaccga aggaggccgt ggggctctgc aaggcgcaga tcactgccat 3061 catctctcag caaggtgaca tatttgtttt tgacctggag acctcagctg tcgctccctt 3121 tgtttggttg gatgtaggaa gcatcccagg gagatttagt gacaatggtt tcctcatgac 3181 tgagaagaca cgaactatat tattttaccc ttgggagccc accagcaaga atgagttgga 3241 gcaatctttt catgtgacct ccttaacaga tatttactga CCAGCTTTCT TGTACAAAGT 3301 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 3361 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 3421 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 3481 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 3541 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 3601 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 3661 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 3721 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 3781 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 3841 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 3901 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 3961 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 4021 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 4081 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 4141 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 4201 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 4261 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 4321 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 4381 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 4441 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 4501 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 4561 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 4621 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 4681 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 4741 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 4801 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 4861 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 4921 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 4981 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 5041 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 5101 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //