LOCUS HEXA-pGEc2-DEST 6808 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6286..6415) /note="3'-ITR" misc_feature complement(5984..6198) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5436..5977 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4615..4637 /note="attB2" misc_feature 5415..5420 /note="BstB1 site" misc_feature 4638..5333 /note="Super GFP" misc_feature 5334..5384 /note="AviTag" misc_feature 5385..5408 /note="His Tag" PCR_frame 3002..4615 /note="Reading Frame" /set="Set 1" source 3006..4592 /dnas_title="HEXA-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:14125 IMAGE:3353424" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4592 /dnas_title="HEXA" /gene="HEXA" /gene_synonym="TSD" /db_xref="GeneID:3073" /db_xref="HGNC:4878" /db_xref="MIM:606869" CDS 3006..4592 /dnas_title="hexosaminidase A (alpha polypeptide)" /gene="HEXA" /gene_synonym="TSD" /codon_start=1 /product="hexosaminidase A (alpha polypeptide)" /protein_id="AAH18927.1" /db_xref="GI:17511941" /db_xref="GeneID:3073" /db_xref="HGNC:4878" /db_xref="MIM:606869" /translation="MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNF QFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTP GCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTE IEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTF PELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTP CYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPE IQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVW REDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAFEGTPEQKA LVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELL RRGVQAQPLNVGFCEQEFEQT" misc_difference 3014..3014 /dnas_title="HEXA" /gene="HEXA" /gene_synonym="TSD" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3006..4592 /note="HEXA coding region" misc_difference 4311..4311 /dnas_title="HEXA" /gene="HEXA" /gene_synonym="TSD" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome." misc_feature 4593..4613 /note="TEV site" misc_difference 4523..4523 /dnas_title="HEXA" /gene="HEXA" /gene_synonym="TSD" /note="'G' in cDNA is 'A' in the human genome; no amino acid change." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4622) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4622) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4623..6808,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4622 /source="HEXA-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgac aagttccagg ctttggtttt cgctgctgct ggcggcagcg ttcgcaggac 3061 gggcgacggc cctctggccc tggcctcaga acttccaaac ctccgaccag cgctacgtcc 3121 tttacccgaa caactttcaa ttccagtacg atgtcagctc ggccgcgcag cccggctgct 3181 cagtcctcga cgaggccttc cagcgctatc gtgacctgct tttcggttcc gggtcttggc 3241 cccgtcctta cctcacaggg aaacggcata cactggagaa gaatgtgttg gttgtctctg 3301 tagtcacacc tggatgtaac cagcttccta ctttggagtc agtggagaat tataccctga 3361 ccataaatga tgaccagtgt ttactcctct ctgagactgt ctggggagct ctccgaggtc 3421 tggagacttt tagccagctt gtttggaaat ctgctgaggg cacattcttt atcaacaaga 3481 ctgagattga ggactttccc cgctttcctc accggggctt gctgttggat acatctcgcc 3541 attacctgcc actctctagc atcctggaca ctctggatgt catggcgtac aataaattga 3601 acgtgttcca ctggcatctg gtagatgatc cttccttccc atatgagagc ttcacttttc 3661 cagagctcat gagaaagggg tcctacaacc ctgtcaccca catctacaca gcacaggatg 3721 tgaaggaggt cattgaatac gcacggctcc ggggtatccg tgtgcttgca gagtttgaca 3781 ctcctggcca cactttgtcc tggggaccag gtatccctgg attactgact ccttgctact 3841 ctgggtctga gccctctggc acctttggac cagtgaatcc cagtctcaat aatacctatg 3901 agttcatgag cacattcttc ttagaagtca gctctgtctt cccagatttt tatcttcatc 3961 ttggaggaga tgaggttgat ttcacctgct ggaagtccaa cccagagatc caggacttta 4021 tgaggaagaa aggcttcggt gaggacttca agcagctgga gtccttctac atccagacgc 4081 tgctggacat cgtctcttct tatggcaagg gctatgtggt gtggcaggag gtgtttgata 4141 ataaagtaaa gattcagcca gacacaatca tacaggtgtg gcgagaggat attccagtga 4201 actatatgaa ggagctggaa ctggtcacca aggccggctt ccgggccctt ctctctgccc 4261 cctggtacct gaaccgtata tcctatggcc ctgactggaa ggatttctac gtagtggaac 4321 ccctggcatt tgaaggtacc cctgagcaga aggctctggt gattggtgga gaggcttgta 4381 tgtggggaga atatgtggac aacacaaacc tggtccccag gctctggccc agagcagggg 4441 ctgttgccga aaggctgtgg agcaacaagt tgacatctga cctgacattt gcctatgaac 4501 gtttgtcaca cttccgctgt gagttgctga ggcgaggtgt ccaggcccaa cccctcaatg 4561 taggcttctg tgagcaggag tttgaacaga ccgaaaactt gtactttcaa ggctCCAGCT 4621 TTCTTGTACA AAGTGGTATG AGCAAGGGCG AGGAACTGTT CACCGGCGTG GTGCCCATCC 4681 TGGTGGAACT GGATGGCGAC GTGAACGGCC ACAAGTTCTC TGTGCGGGGA GAGGGCGAAG 4741 GCGACGCCAC AAATGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG 4801 TGCCTTGGCC TACCCTCGTG ACCACACTGA CCTACGGCGT GCAGTGCTTC AGCAGATACC 4861 CCGACCATAT GAAGCGGCAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG 4921 AACGGACCAT CAGCTTCAAG GACGACGGCA CCTACAAGAC CAGAGCCGAA GTGAAGTTCG 4981 AGGGCGACAC CCTCGTGAAC CGGATCGAGC TGAAGGGCAT CGATTTCAAA GAGGACGGCA 5041 ACATCCTGGG CCACAAGCTG GAGTACAACT TCAACAGCCA CAACGTGTAC ATCACCGCCG 5101 ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG GCACAACGTG GAAGATGGCA 5161 GCGTGCAGCT GGCCGACCAC TACCAGCAGA ACACCCCCAT CGGAGATGGC CCCGTGCTGC 5221 TGCCCGACAA CCACTACCTG AGCACCCAGA GCGTGCTGAG CAAGGACCCC AACGAGAAGC 5281 GGGACCACAT GGTGCTGCTG GAATTCGTGA CCGCCGCTGG CATCACCCAC GGCATGAGCG 5341 GCCTGAACGA CATCTTCGAG GCCCAGAAAA TCGAGTGGCA CGAGCACCAC CATCATCACC 5401 ATCACCACTG ATGATTCGAA AAATAATAAG GATCCAATCA ACCTCTGGAT TACAAAATTT 5461 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 5521 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 5581 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 5641 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 5701 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 5761 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 5821 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 5881 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 5941 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC 6001 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 6061 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 6121 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 6181 TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC GATTAGGATC TTCCTAGAGC 6241 ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA 6301 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG 6361 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA 6421 ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC GCCAATTCGC CCTATAGTGA 6481 GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 6541 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 6601 AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAG 6661 CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 6721 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 6781 TAACAAAATA TTAACGCTTA CAATTTAG //