LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2282..2378) /label=attL2 /note="attL2 (c)" misc_signal complement(2393..2412) /note="T7 Promoter/priming site (c)" primer_bind 2420..2436 /note="M13 Reverse priming site" gene 2549..3358 /note="Kanamycin resistance gene" rep_origin 3479..4152 /note="pUC origin" PCR_frame 669..2282 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2302) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2299) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2290..4155,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2260..2280 /note="TEV site" source 673..2259 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:14125 IMAGE:3353424" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2259 /gene="HEXA" /gene_synonym=TSD /db_xref="GeneID:3073" /db_xref="HGNC:4878" /db_xref="MIM:606869" CDS 673..2259 /dnas_title="hexosaminidase A (alpha polypeptide)" /gene="HEXA" /gene_synonym=TSD /codon_start=1 /product="hexosaminidase A (alpha polypeptide)" /protein_id="AAH18927.1" /db_xref="GI:17511941" /db_xref="GeneID:3073" /db_xref="HGNC:4878" /db_xref="MIM:606869" /translation="MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNF QFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTP GCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTE IEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTF PELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTP CYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPE IQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVW REDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAFEGTPEQKA LVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELL RRGVQAQPLNVGFCEQEFEQT" misc_difference 681..681 /gene="HEXA" /gene_synonym=TSD /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2259 /note="HEXA coding region" misc_difference 1978..1978 /gene="HEXA" /gene_synonym=TSD /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome." misc_difference 2190..2190 /gene="HEXA" /gene_synonym=TSD /note="'G' in cDNA is 'A' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgacaag ttccaggctt tggttttcgc tgctgctggc ggcagcgttc 721 gcaggacggg cgacggccct ctggccctgg cctcagaact tccaaacctc cgaccagcgc 781 tacgtccttt acccgaacaa ctttcaattc cagtacgatg tcagctcggc cgcgcagccc 841 ggctgctcag tcctcgacga ggccttccag cgctatcgtg acctgctttt cggttccggg 901 tcttggcccc gtccttacct cacagggaaa cggcatacac tggagaagaa tgtgttggtt 961 gtctctgtag tcacacctgg atgtaaccag cttcctactt tggagtcagt ggagaattat 1021 accctgacca taaatgatga ccagtgttta ctcctctctg agactgtctg gggagctctc 1081 cgaggtctgg agacttttag ccagcttgtt tggaaatctg ctgagggcac attctttatc 1141 aacaagactg agattgagga ctttccccgc tttcctcacc ggggcttgct gttggataca 1201 tctcgccatt acctgccact ctctagcatc ctggacactc tggatgtcat ggcgtacaat 1261 aaattgaacg tgttccactg gcatctggta gatgatcctt ccttcccata tgagagcttc 1321 acttttccag agctcatgag aaaggggtcc tacaaccctg tcacccacat ctacacagca 1381 caggatgtga aggaggtcat tgaatacgca cggctccggg gtatccgtgt gcttgcagag 1441 tttgacactc ctggccacac tttgtcctgg ggaccaggta tccctggatt actgactcct 1501 tgctactctg ggtctgagcc ctctggcacc tttggaccag tgaatcccag tctcaataat 1561 acctatgagt tcatgagcac attcttctta gaagtcagct ctgtcttccc agatttttat 1621 cttcatcttg gaggagatga ggttgatttc acctgctgga agtccaaccc agagatccag 1681 gactttatga ggaagaaagg cttcggtgag gacttcaagc agctggagtc cttctacatc 1741 cagacgctgc tggacatcgt ctcttcttat ggcaagggct atgtggtgtg gcaggaggtg 1801 tttgataata aagtaaagat tcagccagac acaatcatac aggtgtggcg agaggatatt 1861 ccagtgaact atatgaagga gctggaactg gtcaccaagg ccggcttccg ggcccttctc 1921 tctgccccct ggtacctgaa ccgtatatcc tatggccctg actggaagga tttctacgta 1981 gtggaacccc tggcatttga aggtacccct gagcagaagg ctctggtgat tggtggagag 2041 gcttgtatgt ggggagaata tgtggacaac acaaacctgg tccccaggct ctggcccaga 2101 gcaggggctg ttgccgaaag gctgtggagc aacaagttga catctgacct gacatttgcc 2161 tatgaacgtt tgtcacactt ccgctgtgag ttgctgaggc gaggtgtcca ggcccaaccc 2221 ctcaatgtag gcttctgtga gcaggagttt gaacagaccg aaaacttgta ctttcaaggc 2281 tCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2341 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2401 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2461 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2521 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2581 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2641 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2701 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2761 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2821 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2881 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2941 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 3001 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 3061 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 3121 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3181 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3241 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3301 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3361 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3421 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3481 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3541 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3601 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3661 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3721 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3781 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3841 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3901 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3961 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 4021 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 4081 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4141 TTTTGCTCAC ATGTT //