LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2216..2312) /label=attL2 /note="attL2 (c)" misc_signal complement(2327..2346) /note="T7 Promoter/priming site (c)" primer_bind 2354..2370 /note="M13 Reverse priming site" gene 2483..3292 /note="Kanamycin resistance gene" rep_origin 3413..4086 /note="pUC origin" vector join(2224..4089,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2215 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:14125 IMAGE:3353424" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2215 /gene="HEXA" /gene_synonym=TSD /db_xref="GeneID:3073" /db_xref="HGNC:4878" /db_xref="MIM:606869" CDS 692..2215 /dnas_title="hexosaminidase A (alpha polypeptide)" /gene="HEXA" /gene_synonym=TSD /codon_start=1 /product="hexosaminidase A (alpha polypeptide)" /protein_id="AAH18927.1" /db_xref="GI:17511941" /db_xref="GeneID:3073" /db_xref="HGNC:4878" /db_xref="MIM:606869" /translation="MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNF QFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTP GCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTE IEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTF PELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTP CYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPE IQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVW REDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAFEGTPEQKA LVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELL RRGVQAQPLNVGFCEQEFEQT" misc_feature 692..2215 /note="HEXA coding region" misc_difference 1931..1931 /gene="HEXA" /gene_synonym=TSD /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome." misc_difference 2143..2143 /gene="HEXA" /gene_synonym=TSD /note="'G' in cDNA is 'A' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cctctggccc tggcctcaga acttccaaac 721 ctccgaccag cgctacgtcc tttacccgaa caactttcaa ttccagtacg atgtcagctc 781 ggccgcgcag cccggctgct cagtcctcga cgaggccttc cagcgctatc gtgacctgct 841 tttcggttcc gggtcttggc cccgtcctta cctcacaggg aaacggcata cactggagaa 901 gaatgtgttg gttgtctctg tagtcacacc tggatgtaac cagcttccta ctttggagtc 961 agtggagaat tataccctga ccataaatga tgaccagtgt ttactcctct ctgagactgt 1021 ctggggagct ctccgaggtc tggagacttt tagccagctt gtttggaaat ctgctgaggg 1081 cacattcttt atcaacaaga ctgagattga ggactttccc cgctttcctc accggggctt 1141 gctgttggat acatctcgcc attacctgcc actctctagc atcctggaca ctctggatgt 1201 catggcgtac aataaattga acgtgttcca ctggcatctg gtagatgatc cttccttccc 1261 atatgagagc ttcacttttc cagagctcat gagaaagggg tcctacaacc ctgtcaccca 1321 catctacaca gcacaggatg tgaaggaggt cattgaatac gcacggctcc ggggtatccg 1381 tgtgcttgca gagtttgaca ctcctggcca cactttgtcc tggggaccag gtatccctgg 1441 attactgact ccttgctact ctgggtctga gccctctggc acctttggac cagtgaatcc 1501 cagtctcaat aatacctatg agttcatgag cacattcttc ttagaagtca gctctgtctt 1561 cccagatttt tatcttcatc ttggaggaga tgaggttgat ttcacctgct ggaagtccaa 1621 cccagagatc caggacttta tgaggaagaa aggcttcggt gaggacttca agcagctgga 1681 gtccttctac atccagacgc tgctggacat cgtctcttct tatggcaagg gctatgtggt 1741 gtggcaggag gtgtttgata ataaagtaaa gattcagcca gacacaatca tacaggtgtg 1801 gcgagaggat attccagtga actatatgaa ggagctggaa ctggtcacca aggccggctt 1861 ccgggccctt ctctctgccc cctggtacct gaaccgtata tcctatggcc ctgactggaa 1921 ggatttctac gtagtggaac ccctggcatt tgaaggtacc cctgagcaga aggctctggt 1981 gattggtgga gaggcttgta tgtggggaga atatgtggac aacacaaacc tggtccccag 2041 gctctggccc agagcagggg ctgttgccga aaggctgtgg agcaacaagt tgacatctga 2101 cctgacattt gcctatgaac gtttgtcaca cttccgctgt gagttgctga ggcgaggtgt 2161 ccaggcccaa cccctcaatg taggcttctg tgagcaggag tttgaacaga cctgaCCAGC 2221 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2281 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2341 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2401 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2461 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2521 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2581 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2641 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2701 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2761 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2821 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2881 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2941 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 3001 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 3061 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 3121 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3181 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3241 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3301 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3361 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3421 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3481 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3541 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3601 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3661 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3721 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3781 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3841 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3901 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3961 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 4021 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4081 TCACATGTT //