LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2363..2459) /label=attL2 /note="attL2 (c)" misc_signal complement(2474..2493) /note="T7 Promoter/priming site (c)" primer_bind 2501..2517 /note="M13 Reverse priming site" gene 2630..3439 /note="Kanamycin resistance gene" rep_origin 3560..4233 /note="pUC origin" PCR_frame 669..2363 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2383) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2380) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2371..4236,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2341..2361 /note="TEV site" source 673..2340 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1725 IMAGE:2967035" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2340 /gene="HEXB" /gene_synonym=ENC-1AS /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" CDS 673..2340 /dnas_title="hexosaminidase B (beta polypeptide)" /gene="HEXB" /gene_synonym=ENC-1AS /codon_start=1 /product="hexosaminidase B (beta polypeptide)" /protein_id="AAH17378.1" /db_xref="GI:16924217" /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" /translation="MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAAR APSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYH GYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVL KANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKI ILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIE YARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLT TFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVL DIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWY LDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASA VGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM" misc_difference 857..857 /gene="HEXB" /gene_synonym=ENC-1AS /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 673..2340 /note="HEXB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggagct gtgcgggctg gggctgcccc ggccgcccat gctgctggcg 721 ctgctgttgg cgacactgct ggcggcgatg ttggcgctgc tgactcaggt ggcgctggtg 781 gtgcaggtgg cggaggcggc tcgggccccg agcgtctcgg ccaagccggg gccggcgctg 841 tggcccctgc cgctctcggt gaagatgacc ccgaacctgc tgcatctcgc cccggagaac 901 ttctacatca gccacagccc caattccacg gcgggcccct cctgcaccct gctggaggaa 961 gcgtttcgac gatatcatgg ctatattttt ggtttctaca agtggcatca tgaacctgct 1021 gaattccagg ctaaaaccca ggttcagcaa cttcttgtct caatcaccct tcagtcagag 1081 tgtgatgctt tccccaacat atcttcagat gagtcttata ctttacttgt gaaagaacca 1141 gtggctgtcc ttaaggccaa cagagtttgg ggagcattac gaggtttaga gacctttagc 1201 cagttagttt atcaagattc ttatggaact ttcaccatca atgaatccac cattattgat 1261 tctccaaggt tttctcacag aggaattttg attgatacat ccagacatta tctgccagtt 1321 aagattattc ttaaaactct ggatgccatg gcttttaata agtttaatgt tcttcactgg 1381 cacatagttg atgaccagtc tttcccatat cagagcatca cttttcctga gttaagcaat 1441 aaaggaagct attctttgtc tcatgtttat acaccaaatg atgtccgtat ggtgattgaa 1501 tatgccagat tacgaggaat tcgagtcctg ccagaatttg atacccctgg gcatacacta 1561 tcttggggaa aaggtcagaa agacctcctg actccatgtt acagtagaca aaacaagttg 1621 gactcttttg gacctataaa ccctactctg aatacaacat acagcttcct tactacattt 1681 ttcaaagaaa ttagtgaggt gtttccagat caattcattc atttgggagg agatgaagtg 1741 gaatttaaat gttgggaatc aaatccaaaa attcaagatt tcatgaggca aaaaggcttt 1801 ggcacagatt ttaagaaact agaatctttc tacattcaaa aggttttgga tattattgca 1861 accataaaca agggatccat tgtctggcag gaggtttttg atgataaagc aaagcttgcg 1921 ccgggcacaa tagttgaagt atggaaagac agcgcatatc ctgaggaact cagtagagtc 1981 acagcatctg gcttccctgt aatcctttct gctccttggt acttagattt gattagctat 2041 ggacaagatt ggaggaaata ctataaagtg gaacctcttg attttggcgg tactcagaaa 2101 cagaaacaac ttttcattgg tggagaagct tgtctatggg gagaatatgt ggatgcaact 2161 aacctcactc caagattatg gcctcgggca agtgctgttg gtgagagact ctggagttcc 2221 aaagatgtca gagatatgga tgacgcctat gacagactga caaggcaccg ctgcaggatg 2281 gtcgaacgtg gaatagctgc acaacctctt tatgctggat attgtaacca tgagaacatg 2341 gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2401 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2461 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2521 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2581 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2641 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2701 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2761 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2821 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2881 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2941 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 3001 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 3061 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 3121 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3181 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3241 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3301 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3361 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3421 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3481 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3541 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3601 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3661 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3721 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3781 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3841 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3901 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3961 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 4021 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 4081 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 4141 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4201 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //