LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2237..2333) /label=attL2 /note="attL2 (c)" misc_signal complement(2348..2367) /note="T7 Promoter/priming site (c)" primer_bind 2375..2391 /note="M13 Reverse priming site" gene 2504..3313 /note="Kanamycin resistance gene" rep_origin 3434..4107 /note="pUC origin" vector join(2245..4110,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2236 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1725 IMAGE:2967035" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2236 /gene="HEXB" /gene_synonym=ENC-1AS /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" CDS 692..2236 /dnas_title="hexosaminidase B (beta polypeptide)" /gene="HEXB" /gene_synonym=ENC-1AS /codon_start=1 /product="hexosaminidase B (beta polypeptide)" /protein_id="AAH17378.1" /db_xref="GI:16924217" /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" /translation="MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAAR APSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYH GYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVL KANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKI ILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIE YARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLT TFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVL DIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWY LDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASA VGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM" misc_difference 750..750 /gene="HEXB" /gene_synonym=ENC-1AS /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 692..2236 /note="HEXB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgctcgggcc ccgagcgtct cggccaagcc 721 ggggccggcg ctgtggcccc tgccgctctc ggtgaagatg accccgaacc tgctgcatct 781 cgccccggag aacttctaca tcagccacag ccccaattcc acggcgggcc cctcctgcac 841 cctgctggag gaagcgtttc gacgatatca tggctatatt tttggtttct acaagtggca 901 tcatgaacct gctgaattcc aggctaaaac ccaggttcag caacttcttg tctcaatcac 961 ccttcagtca gagtgtgatg ctttccccaa catatcttca gatgagtctt atactttact 1021 tgtgaaagaa ccagtggctg tccttaaggc caacagagtt tggggagcat tacgaggttt 1081 agagaccttt agccagttag tttatcaaga ttcttatgga actttcacca tcaatgaatc 1141 caccattatt gattctccaa ggttttctca cagaggaatt ttgattgata catccagaca 1201 ttatctgcca gttaagatta ttcttaaaac tctggatgcc atggctttta ataagtttaa 1261 tgttcttcac tggcacatag ttgatgacca gtctttccca tatcagagca tcacttttcc 1321 tgagttaagc aataaaggaa gctattcttt gtctcatgtt tatacaccaa atgatgtccg 1381 tatggtgatt gaatatgcca gattacgagg aattcgagtc ctgccagaat ttgatacccc 1441 tgggcataca ctatcttggg gaaaaggtca gaaagacctc ctgactccat gttacagtag 1501 acaaaacaag ttggactctt ttggacctat aaaccctact ctgaatacaa catacagctt 1561 ccttactaca tttttcaaag aaattagtga ggtgtttcca gatcaattca ttcatttggg 1621 aggagatgaa gtggaattta aatgttggga atcaaatcca aaaattcaag atttcatgag 1681 gcaaaaaggc tttggcacag attttaagaa actagaatct ttctacattc aaaaggtttt 1741 ggatattatt gcaaccataa acaagggatc cattgtctgg caggaggttt ttgatgataa 1801 agcaaagctt gcgccgggca caatagttga agtatggaaa gacagcgcat atcctgagga 1861 actcagtaga gtcacagcat ctggcttccc tgtaatcctt tctgctcctt ggtacttaga 1921 tttgattagc tatggacaag attggaggaa atactataaa gtggaacctc ttgattttgg 1981 cggtactcag aaacagaaac aacttttcat tggtggagaa gcttgtctat ggggagaata 2041 tgtggatgca actaacctca ctccaagatt atggcctcgg gcaagtgctg ttggtgagag 2101 actctggagt tccaaagatg tcagagatat ggatgacgcc tatgacagac tgacaaggca 2161 ccgctgcagg atggtcgaac gtggaatagc tgcacaacct ctttatgctg gatattgtaa 2221 ccatgagaac atgtaaCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 2281 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 2341 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 2401 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 2461 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 2521 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 2581 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 2641 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 2701 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 2761 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 2821 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 2881 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 2941 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 3001 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 3061 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 3121 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 3181 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 3241 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 3301 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 3361 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 3421 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 3481 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 3541 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3601 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3661 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3721 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3781 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3841 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 3901 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 3961 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 4021 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 4081 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //